Characterization of a natural mouse monoclonal antibody recognizing epitopes shared by oxidized low-density lipoprotein and chaperonin 60 of Aggregatibacter actinomycetemcomitans

2016 ◽  
Vol 64 (3) ◽  
pp. 699-710 ◽  
Author(s):  
Chunguang Wang ◽  
Jari Kankaanpää ◽  
Outi Kummu ◽  
S. Pauliina Turunen ◽  
Ramin Akhi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Mouse Monoclonal Antibody ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein ◽  
Chaperonin 60

Screening, expression, and characterization of an anti-human oxidized low-density lipoprotein single-chain variable fragment

2016 ◽  
Vol 122 (3) ◽  
pp. 287-293 ◽  
Author(s):  
Miyuki Kumano-Kuramochi ◽  
Takashi Fujimura ◽  
Shiro Komba ◽  
Mari Maeda-Yamamoto ◽  
Sachiko Machida
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Single Chain Variable Fragment ◽  
Oxidized Low Density Lipoprotein ◽  
Single Chain

Immunochemical Characterization of Purified Human Oxidized Low-Density Lipoprotein Antibodies

2000 ◽  
Vol 95 (2) ◽  
pp. 135-144 ◽  
Author(s):  
G. Virella ◽  
S. Koskinen ◽  
G. Krings ◽  
J.M. Onorato ◽  
S.R. Thorpe ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein ◽  
Immunochemical Characterization

Characterization of peroxynitrite-oxidized low density lipoprotein binding to human CD36

2001 ◽  
Vol 155 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Rebecca A. Guy ◽  
Graham F. Maguire ◽  
Ian Crandall ◽  
Philip W. Connelly ◽  
Kevin C. Kain
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein ◽  
Lipoprotein Binding

scholarly journals The terminal six amino-acids of the carboxy cytoplasmic tail of CD36 contain a functional domain implicated in the binding and capture of oxidized low-density lipoprotein

2002 ◽  
Vol 364 (2) ◽  
pp. 507-515 ◽  
Author(s):  
Eric MALAUD ◽  
Delphine HOURTON ◽  
Louise Marie GIROUX ◽  
Ewa NINIO ◽  
Robin BUCKLAND ◽  
...  
Keyword(s):  
Amino Acids ◽  
Monoclonal Antibody ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Cytoplasmic Tail ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Low Density ◽  
Wild Type ◽  
Oxidized Low Density Lipoprotein

CD36, a major adhesion molecule expressed by monocytes/macrophages, plays a key role in the binding and internalization of oxidized low-density lipoprotein (OxLDL). This adhesion molecule, a member of an important scavenger receptor family, contains a very short C-terminal cytoplasmic tail that is known to induce intracellular signalling events. However, the domains on the cytoplasmic tail involved in such signal transduction are unknown. In this study, we have investigated the functional components of the cytoplasmic tail by site-directed mutagenesis coupled with functional OxLDL and monoclonal antibody (mAb) binding studies. Seven truncated or punctual CD36 constructs, localized in the cytoplasmic tail, were produced by site-directed mutagenesis. Each construct was stably expressed in HEK293 cells. We used a quantitative and a qualitative method, labelling OxLDL with either iodine or rhodamine, to determine the functional importance of the cytoplasmic domains in OxLDL internalization. Results indicate that: (1) a deletion of the last amino-acid (construct K472STOP) significantly reduces, compared with wild-type, the binding, internalization and degradation of OxLDL; (2) truncation of the last six amino-acids (construct R467STOP) significantly reduces OxLDL binding; (3) the above two constructs (K472STOP and R467STOP) showed a reduced rate of OxLDL internalization compared with wild-type; (4) the binding and rate of internalization of an anti-CD36 monoclonal antibody (10/5) was not affected by the above mentioned mutants (K472STOP and R467STOP), compared with wild-type. This study shows, for the first time, a specific site on the CD36 cytoplasmic tail that is critical for the binding, endocytosis and targeting of OxLDL.

scholarly journals Characterization of the inhibition of hepatitis C virus entry byIn vitro-generated and patient-derived oxidized low-density lipoprotein

Hepatology ◽  
2013 ◽  
Vol 57 (5) ◽  
pp. 1716-1724 ◽  
Author(s):  
Sandra Westhaus ◽  
Dorothea Bankwitz ◽  
Stefanie Ernst ◽  
Katrin Rohrmann ◽  
Ilka Wappler ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Entry ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein
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