Primordial germ cells can be differentiated by retinoic acid and progesterone induction from embryonic stem cells

2021 ◽  
Vol 46 (3) ◽  
Author(s):  
Meysam Hassani Moghaddam ◽  
Neda Eskandari ◽  
Hossein Nikzad ◽  
Mohammad Miryounesi ◽  
Mohammad Karimian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem

The combination of retinoic acid and estrogen can increase germ cells genes expression in mouse embryonic stem cells derived primordial germ cells

Biologicals ◽  
2018 ◽  
Vol 56 ◽  
pp. 39-44 ◽  
Author(s):  
Neda Eskandari ◽  
Meysam Hassani Moghaddam ◽  
Mohammad Ali Atlasi ◽  
Javad Amini Mahabadi ◽  
Aliakbar Taherian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Genes Expression

scholarly journals CHIR99021 combined with retinoic acid promotes the differentiation of primordial germ cells from human embryonic stem cells

Oncotarget ◽  
2016 ◽  
Vol 8 (5) ◽  
pp. 7814-7826 ◽  
Author(s):  
Tingting Cheng ◽  
Kui Zhai ◽  
Yan Chang ◽  
Guidong Yao ◽  
Jiahuan He ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem

Retinoic acid induces differentiation of buffalo (Bubalus bubalis) embryonic stem cells into germ cells

Gene ◽  
2017 ◽  
Vol 631 ◽  
pp. 54-67 ◽  
Author(s):  
Syed Mohmad Shah ◽  
Suresh Kumar Singla ◽  
Prabhat Palta ◽  
Radhey Sham Manik ◽  
Manmohan Singh Chauhan
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Embryonic Stem ◽  
Bubalus Bubalis

Collagen-alginate microspheres as a 3D culture system for mouse embryonic stem cells differentiation to primordial germ cells

Biologicals ◽  
2017 ◽  
Vol 48 ◽  
pp. 114-120 ◽  
Author(s):  
Vahid Mansouri ◽  
Mohammad Salehi ◽  
Mir davood Omrani ◽  
Zahra Niknam ◽  
Abdolreza Ardeshirylajimi
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Culture System ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
3D Culture ◽  
Mouse Embryonic Stem Cells ◽  
3D Culture System

Retinoic acid induces differentiation of buffalo ( Bubalus bubalis ) embryonic stem cells into germ cells

Gene ◽  
2017 ◽  
Vol 626 ◽  
pp. 358-366 ◽  
Author(s):  
Syed Mohmad Shah ◽  
Suresh Kumar Singla ◽  
Prabhat Palta ◽  
Radhey Sham Manik ◽  
Manmohan Singh Chauhan
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Embryonic Stem ◽  
Bubalus Bubalis

Erratum to Retinoic acid induces differentiation of buffalo (Bubalus bubalis) embryonic stem cells into germ cells [GENE 626C (2017) 358–366]

Gene ◽  
2017 ◽  
Vol 631 ◽  
pp. 53
Author(s):  
Syed Mohmad Shah ◽  
Suresh Kumar Singla ◽  
Prabhat Palta ◽  
Radhey Sham Manik ◽  
Manmohan Singh Chauhan
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Embryonic Stem ◽  
Bubalus Bubalis

An Evidence That Murine Marrow-Derived CXCR4+ SSEA-1+ Oct-4+ Very Small Embryonic-Like (VSEL) Stem Cells Are Pluripotent and Express Several Primordial Germ Cell (PGC) Markers - Hypotesis for Developmental Deposition of PGC in Various Organs.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1676-1676 ◽  
Author(s):  
Magda Kucia ◽  
Ewa Zuba-Surma ◽  
Ryan Reca ◽  
Janina Ratajczak ◽  
Mariusz Ratajczak
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Fetal Liver ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
C2c12 Cells

Abstract Recently we identified in murine BM a homogenous population of rare (~0.01% of BMMNC) Sca-1+ lin− CD45− cells that express by RQ-PCR and immunhistochemistry markers of pluripotent stem cells (PSC) such as SSEA-1, Oct-4, Nanog and Rex-1 and highly express Rif-1 telomerase protein (Leukemia2006;20,857–869). Direct electronmicroscopical analysis revealed that these cells display several features typical for embryonic stem cells such as i) small size (2–4 um in diameter), ii) large nuclei surrounded by a narrow rim of cytoplasm, and iii) open-type chromatin (euchromatin). We also found that VSELs may be released from BM and circulate in peripheral blood during tissue/organ injuries (e.g., heart infarct, stroke). Recently we noticed that ~5–10% of purified VSELs if plated over a C2C12 murine sarcoma cell feeder layer are able to form spheres that resemble embryoid bodies. Cells from these VSEL-derived spheres (VSEL-DS) are composed of immature cells with large nuclei containing euchromatin, and similarly as purified VSELs are CXCR4+SSEA-1+Oct-4+. Furthermore, VSEL-DS after replating over C2C12 cells may again (up to 5–7 passages) grow new spheres or if plated into cultures promoting tissue differentiation expand into cells from all three germ-cell layers. The formation of VSEL-DS was observed in a presence of C2C12 cells obtained from different sources. Furthermore, VSELs isolated from GFP+ mice grew GFP+ VSEL-DS which show a diploid content of DNA. This suggests that VSEL-DS are in fact derived from VSELs and not from the supportive C2C12 cell line as well as excludes the possibility of cell fusion to the observed phenomenon. Similar spheres were also formed by VSELs isolated from murine fetal liver, spleen and thymus. Interestingly formation of VSEL-DS was associated with a young age, and no VSEL-DS were observed by cells isolated from old mice (> 2 years). We also found that cells isolated from VSEL-DS similarly as embryonic stem cells grow tumors after injection into immunodeficient NOD/SCID mice (51/52 inoculated mice). Since VSELs isolated by us express several markers of primordial germ cells (fetal-type alkaline phosphatase, Oct-4, SSEA-1, CXCR4, Mvh, Stella, Fragilis, Nobox, Hdac6) we hypothesize that VSELs are closely related to a population of primordial germ cells. These cells are specified during early gastrulation in the proximal epiblast and subsequently migrate in a CXCR4-SDF-1 dependent manner through the embryo proper to their final destination in genital ridges. It is possible that some of these cells or a population of cells closely related to them migrate astray being chemoattracted by SDF-1 to fetal liver and subsequently, during the third trimester of gestation seed together with hematopoietic stem cells in bone marrow and perhaps other organs as well. In conclusion, we postulate that VSELs identified by us and purified at the single cell level could become an important source of pluripotent stem cells for regeneration.

Primordial germ cells differentiated from embryonic stem cells as a system for investigating transgenerational epigenetic toxicity in vitro

Toxicology ◽  
2011 ◽  
Vol 290 (2-3) ◽  
pp. 109-110
Author(s):  
Emma L. Marczylo ◽  
Justyna Janus ◽  
Kate M. Dudek ◽  
Timothy W. Gant
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Toxicity In Vitro

Comparative transcript profiles of cell cycle-related genes in mouse primordial germ cells, embryonic stem cells and embryonic germ cells

2007 ◽  
Vol 7 (6) ◽  
pp. 714-721 ◽  
Author(s):  
Eleonora Sorrentino ◽  
Valeria Nazzicone ◽  
Donatella Farini ◽  
Luisa Campagnolo ◽  
Massimo De Felici
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Embryonic Germ Cells ◽  
Transcript Profiles

scholarly journals The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells

2015 ◽  
Vol 38 (2) ◽  
pp. 220-226 ◽  
Author(s):  
Vahid Mansouri ◽  
Mohammad Salehi ◽  
Mohsen Nourozian ◽  
Fatemeh Fadaei ◽  
Reza Mastery Farahani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Nuclear Transfer ◽  
Primordial Germ Cells ◽  
Embryonic Stem
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