The effect of over-expression of rate limiting enzymes on the yield of artemisinin in Artemisia annua

2015 ◽  
Vol 27 (2) ◽  
pp. 311-319 ◽  
Author(s):  
Pravej Alam ◽  
Kamaluddin ◽  
Mahmoud A. Sharaf-Eldin ◽  
Shereen F. Elkholy ◽  
Malik Zainul Abdin
Keyword(s):  
Artemisia Annua ◽  
Over Expression ◽  
Rate Limiting

The stacked over-expression of FPS, CYP71AV1 and CPR genes leads to the increase of artemisinin level in Artemisia annua L.

2012 ◽  
Vol 7 (3) ◽  
pp. 287-295 ◽  
Author(s):  
Yunfei Chen ◽  
Qian Shen ◽  
Yueyue Wang ◽  
Tao Wang ◽  
Shaoyan Wu ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Over Expression ◽  
Artemisia Annua L

Enhanced artemisinin accumulation and metabolic profiling of transgenic Artemisia annua L. plants over-expressing by rate-limiting enzymes from isoprenoid pathway

2014 ◽  
Vol 9 (1) ◽  
pp. 655-665 ◽  
Author(s):  
Pravej Alam ◽  
Kamaluddin ◽  
Mather Ali Khan ◽  
Anis Mohammad ◽  
Riyazudeen Khan ◽  
...  
Keyword(s):  
Metabolic Profiling ◽  
Artemisia Annua ◽  
Isoprenoid Pathway ◽  
Artemisia Annua L ◽  
Rate Limiting

Earlier Flowering Induced by Over-Expression of CO Gene Does Not Accompany Increase of Artemisinin Biosynthesis in Artemisia annua

Plant Biology ◽  
2007 ◽  
Vol 9 (3) ◽  
pp. 442-446 ◽  
Author(s):  
H. Wang ◽  
Y. Liu ◽  
K. Chong ◽  
B. Y. Liu ◽  
H. C. Ye ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Over Expression ◽  
Artemisinin Biosynthesis

scholarly journals Over-expression of the brassinosteroid gene TaDWF4 increases wheat productivity under low and sufficient nitrogen through enhanced carbon assimilation

2021 ◽  
Author(s):  
Matthew John Milner ◽  
Stéphanie M. Swarbreck ◽  
Melanie Craze ◽  
Sarah Bowden ◽  
Howard Griffiths ◽  
...  
Keyword(s):  
Crop Yields ◽  
Agronomic Traits ◽  
Carbon Assimilation ◽  
Fold Increase ◽  
Operating Efficiency ◽  
Over Expression ◽  
Rate Limiting Step ◽  
Marginal Soils ◽  
Use Efficiency ◽  
Rate Limiting

There is a strong pressure to reduce nitrogen (N) fertiliser inputs while maintaining or increasing current cereal crop yields. Brassinosteroids, (BR), are a group of phytohormones essential for plant growth and development, that have been demonstrated to regulate several agronomic traits. DWF4 encodes a cytochrome P450 that catalyses a rate-limiting step in BR synthesis. We show that overexpression of the dominant shoot expressed homoeologue TaDWF4-B in wheat can increase plant productivity by up to 105% under a range of N levels on marginal soils, resulting in increased N use efficiency (NUE). We show that a two to four-fold increase in TaDWF4 transcript levels enhances the responsiveness of genes regulated by N. The productivity increases seen were primarily due to the maintenance of photosystem II operating efficiency and carbon assimilation in plants when grown under limiting N conditions and not an overall increase in photosynthesis capacity. The increased biomass production and yield per plant in TaDWF4 OE lines could be linked to modified carbon partitioning and changes in expression pattern of the growth regulator Target Of Rapamycin, offering a route towards breeding for sustained yield and lower N inputs.

scholarly journals Palmitic acid causes increased dihydroceramide levels when desaturase expression is directly silenced or indirectly lowered by silencing AdipoR2

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Mario Ruiz ◽  
Marcus Henricsson ◽  
Jan Borén ◽  
Marc Pilon
Keyword(s):  
Palmitic Acid ◽  
Unsaturated Fatty Acids ◽  
Cultured Cells ◽  
De Novo ◽  
Strong Increase ◽  
Over Expression ◽  
C Elegans ◽  
Ceramide Synthesis ◽  
Positive Regulators ◽  
Rate Limiting

Abstract Background AdipoR1 and AdipoR2 (AdipoRs) are plasma membrane proteins often considered to act as adiponectin receptors with a ceramidase activity. Additionally, the AdipoRs and their yeast and C. elegans orthologs are emerging as membrane homeostasis regulators that counter membrane rigidification by promoting fatty acid desaturation and incorporation of unsaturated fatty acids into phospholipids, thus restoring fluidity. Methods Using cultured cells, the effects of AdipoR silencing or over-expression on the levels and composition of several sphingolipid classes were examined. Results AdipoR2 silencing in the presence of exogenous palmitic acid potently causes increased levels of dihydroceramides, a ceramide precursor in the de novo ceramide synthesis pathway. Conversely, AdipoR2 over-expression caused a depletion of dihydroceramides. Conclusions The results are consistent with AdipoR2 silencing leading to increased intracellular supply of palmitic acid that in turn leads to increased dihydroceramide synthesis via the rate-limiting serine palmitoyl transferase step. In agreement with this model, inhibiting the desaturase SCD or SREBF1/2 (positive regulators of SCD) also causes a strong increase in dihydroceramide levels.

Over-expression of Rate-Limiting Enzymes to Improve Alkaloid Productivity

2010 ◽  
pp. 95-109 ◽  
Author(s):  
Tomoya Takemura ◽  
Yit-lai Chow ◽  
Takehiko Todokoro ◽  
Takuya Okamoto ◽  
Fumihiko Sato
Keyword(s):  
Over Expression ◽  
Rate Limiting

1442: CXCL12 Over-Expression and Secretion by Aging Fibroblasts Stimulates Human Prostate Epithelial Proliferation in Vitro

2006 ◽  
Vol 175 (4S) ◽  
pp. 466-466
Author(s):  
Jill A. Macoska ◽  
Lesa Begley ◽  
Christine Monteleon ◽  
James W. MacDonald ◽  
Rajal B. Shah
Keyword(s):  
Human Prostate ◽  
Epithelial Proliferation ◽  
Over Expression ◽  
Proliferation In Vitro
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