Characterization of Broad Spectrum Potato Virus Y Resistance in a Solanum tuberosum ssp. andigena-Derived Population and Select Breeding Clones Using Molecular Markers, Grafting, and Field Inoculations

2009 ◽  
Vol 86 (4) ◽  
pp. 286-296 ◽  
Author(s):  
Jonathan L. Whitworth ◽  
Richard G. Novy ◽  
Darren G. Hall ◽  
James M. Crosslin ◽  
Charles R. Brown
Keyword(s):  
Molecular Markers ◽  
Solanum Tuberosum ◽  
Potato Virus ◽  
Broad Spectrum ◽  
Potato Virus Y

Distribution and molecular characterization of potato virus Y (PVY) strains infecting potato (Solanum tuberosum) crop in Kashmir (India)

VirusDisease ◽  
2021 ◽  
Author(s):  
Sumiah Wani ◽  
Sahar Saleem ◽  
Sajad U. Nabi ◽  
Gowhar Ali ◽  
Bilal A. Paddar ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

scholarly journals Detection and genome characterization of Potato virus Y isolates infecting potato (Solanum tuberosum L.) in La Union (Antioquia, Colombia)

2016 ◽  
Vol 34 (3) ◽  
pp. 317-328 ◽  
Author(s):  
Pablo Gutiérrez S. ◽  
Mauricio Marín M. ◽  
Daniel Muñoz E.
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Seed Tuber ◽  
Rt Pcr ◽  
Potato Plants ◽  
Nextgeneration Sequencing ◽  
Prevalent Strain ◽  
Detailed Molecular Analysis

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

Biological and Molecular Characterization of Potato virus Y Infecting Potato (Solanum tuberosum) in India

2013 ◽  
Vol 6 (5) ◽  
pp. 257-264 ◽  
Author(s):  
Pooja Sharma ◽  
Anurag Kumar Sahu ◽  
Rakesh Kumar Verma ◽  
Ritesh Mishra ◽  
R.K. Gaur
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

Molecular Markers of Genes for Extreme Resistance to Potato Virus Y in Solanum tuberosum L. Cultivars and Hybrids

2019 ◽  
Vol 45 (6) ◽  
pp. 517-523
Author(s):  
V. A. Biryukova ◽  
I. V. Shmiglya ◽  
V. A. Zharova ◽  
M. P. Beketova ◽  
E. V. Rogozina ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Solanum Tuberosum L ◽  
Extreme Resistance

A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY).

2005 ◽  
Vol 125 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Emmanuel Jacquot ◽  
Michel Tribodet ◽  
Flora Croizat ◽  
Valérie Balme-Sinibaldi ◽  
Camille Kerlan
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

scholarly journals Arbuscular Mycorrhiza Changes the Impact of Potato Virus Y on Growth and Stress Tolerance of Solanum tuberosum L. in vitro

2020 ◽  
Vol 10 ◽  
Author(s):  
Edyta Deja-Sikora ◽  
Anita Kowalczyk ◽  
Alina Trejgell ◽  
Adriana Szmidt-Jaworska ◽  
Christel Baum ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Arbuscular Mycorrhiza ◽  
Stress Tolerance ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Solanum Tuberosum L ◽  
The Impact

scholarly journals Detection and molecular characterization of Slovak tomato isolates belonging to two recombinant strains of potato virus Y

2016 ◽  
Vol 60 (04) ◽  
pp. 347-353 ◽  
Author(s):  
N. SIHELSKÁ ◽  
L. PREDAJŇA ◽  
A. NAGYOVÁ ◽  
K. ŠOLTYS ◽  
J. BUDIŠ ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Recombinant Strains

Characterization of a Novel Far-Eastern Potato Virus Y Isolates

2009 ◽  
Vol 8 (2) ◽  
pp. 62-67 ◽  
Author(s):  
Yury G. Volkov ◽  
Nadezhda N. Kakareka ◽  
Larissa A. Balabanova ◽  
Zinaida N. Kozlovskay ◽  
Mikhail V. Sapotsky
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Far Eastern
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