Optimized production of 2,3-butanediol by a lactate dehydrogenase-deficient mutant of Klebsiella pneumoniae

2013 ◽  
Vol 18 (6) ◽  
pp. 1210-1215 ◽  
Author(s):  
Sung-Mok Lee ◽  
Baek-Rock Oh ◽  
Jang Min Park ◽  
Anna Yu ◽  
Sun-Yeon Heo ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Klebsiella Pneumoniae ◽  
Deficient Mutant

scholarly journals Identification of Genes Induced In Vivo duringKlebsiella pneumoniae CG43 Infection

2001 ◽  
Vol 69 (11) ◽  
pp. 7140-7145 ◽  
Author(s):  
Yi-Chyi Lai ◽  
Hwei-Ling Peng ◽  
Hwan-You Chang
Keyword(s):  
Protein Folding ◽  
Klebsiella Pneumoniae ◽  
Nutrient Uptake ◽  
Capsular Polysaccharide ◽  
The Other ◽  
Deficient Mutant ◽  
Turn On ◽  
In Vivo Selection ◽  
Iron Deprivation

ABSTRACT A novel in vivo expression technology (IVET) was performed to identify Klebsiella pneumoniae CG43 genes that are specifically expressed during infection of BALB/c mice. The IVET employed a UDP glucose pyrophosphorylase (galU)-deficient mutant of K. pneumoniae which is incapable of utilizing galactose and synthesizing capsular polysaccharide, as demonstrated by its low virulence to BALB/c mice and a white nonmucoid colony morphology on MacConkey-galactose agar. By using a functionalgalU gene as the reporter, an IVE promoter could render thegalU mutant virulent while maintaining the white nonmucoid colony phenotype. A total of 20 distinct sequences were obtained through the in vivo selection. Five of them have been identified previously as virulence-associated genes in other pathogens, while another five with characterized functions are involved in regulation and transportation of nutrient uptake, biosynthesis of isoprenoids, and protein folding. No known functions have been attributed to the other 10 sequences. We have also demonstrated that 2 of the 20 IVE genes turn on under iron deprivation, whereas the expression of another five genes was found to be activated in the presence of paraquat, a superoxide generator.

Enhancement of ethanol production from glycerol in a Klebsiella pneumoniae mutant strain by the inactivation of lactate dehydrogenase

2012 ◽  
Vol 47 (1) ◽  
pp. 156-159 ◽  
Author(s):  
Baek-Rock Oh ◽  
Jeong-Woo Seo ◽  
Sun-Yeon Heo ◽  
Won-Kyung Hong ◽  
Lian Hua Luo ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Klebsiella Pneumoniae ◽  
Ethanol Production ◽  
Mutant Strain

scholarly journals Effect of Porins and Plasmid-Mediated AmpC β-Lactamases on the Efficacy of β-Lactams in Rat Pneumonia Caused by Klebsiella pneumoniae

2006 ◽  
Vol 50 (6) ◽  
pp. 2258-2260 ◽  
Author(s):  
Emma Padilla ◽  
Diana Alonso ◽  
Antonio Doménech-Sánchez ◽  
Cristina Gomez ◽  
José Luis Pérez ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Deficient Mutant ◽  
Better Than

ABSTRACT The in vivo activities of imipenem, meropenem, and cefepime were studied in a model of rat pneumonia caused by a plasmid-mediated AmpC β-lactamase ACT-1-producing Klebsiella pneumoniae strain (K. pneumoniae strain 12) and a derivative porin-deficient mutant (K. pneumoniae strain 12dp). No differences between these activities were seen with K. pneumoniae 12. Only meropenem showed an activity slightly better than that of imipenem with K. pneumoniae 12dp.

A spontaneous lactate dehydrogenase deficient mutant ofStreptococcus rattusfor use as a probiotic in the prevention of dental caries

2009 ◽  
Vol 107 (5) ◽  
pp. 1551-1558 ◽  
Author(s):  
J.D. Hillman ◽  
E. McDonell ◽  
T. Cramm ◽  
C.H. Hillman ◽  
R.T. Zahradnik
Keyword(s):  
Lactate Dehydrogenase ◽  
Dental Caries ◽  
Deficient Mutant
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