COR-101, ein menschlicher Antikörper gegen COVID-19

AbstractCOR-101 is a fully human, Fc silenced IgG that was discovered by antibody phage display. It reduced the SARS-CoV-2 virus load in the lung by more than 99 percent in Hamster models and led to much faster recovery. Its mode of action has been elucidated by solving the atomic structure of its interaction with SARS-CoV-2. The antibody competes with ACE2 binding by blocking a large area of the SARS-CoV-2 spike protein.

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Application of streptavidin mass spectrometric immunoassay tips for immunoaffinity based antibody phage display panning

Journal of Microbiological Methods ◽

10.1016/j.mimet.2015.11.007 ◽

2016 ◽

Vol 120 ◽

pp. 6-14 ◽

Cited By ~ 10

Author(s):

Chai Fung Chin ◽

Lian Wee Ler ◽

Yee Siew Choong ◽

Eugene Boon Beng Ong ◽

Asma Ismail ◽

...

Keyword(s):

Phage Display ◽

Mass Spectrometric ◽

Antibody Phage ◽

Antibody Phage Display

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Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens

Methods in Molecular Biology - Genotype Phenotype Coupling ◽

10.1007/978-1-4939-9853-1_8 ◽

2019 ◽

pp. 143-155 ◽

Cited By ~ 1

Author(s):

Esther V. Wenzel ◽

Kristian D. R. Roth ◽

Giulio Russo ◽

Viola Fühner ◽

Saskia Helmsing ◽

...

Keyword(s):

Phage Display ◽

Antibody Phage ◽

Antibody Phage Display ◽

Phage Display Antibody

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Extremely potent monoclonal antibodies neutralize Omicron and other SARS-CoV-2 variants

10.1101/2022.01.12.22269023 ◽

2022 ◽

Author(s):

Zhaochun Chen ◽

Peng Zhang ◽

Yumiko Matsuoka ◽

Yaroslav Tsybovsky ◽

Kamille West ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Neutralizing Antibodies ◽

Spike Protein ◽

Structural Basis ◽

Hamster Model ◽

Golden Syrian Hamster ◽

Phage Display Libraries ◽

Antibody Phage ◽

Antibody Phage Display ◽

Early Isolate

The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has triggered a devastating global health, social and economic crisis. The RNA nature and broad circulation of this virus facilitate the accumulation of mutations, leading to the continuous emergence of variants of concern with increased transmissibility or pathogenicity1. This poses a major challenge to the effectiveness of current vaccines and therapeutic antibodies1,2. Thus, there is an urgent need for effective therapeutic and preventive measures with a broad spectrum of action, especially against variants with an unparalleled number of mutations such as the recently emerged Omicron variant, which is rapidly spreading across the globe3. Here, we used combinatorial antibody phage-display libraries from convalescent COVID-19 patients to generate monoclonal antibodies against the receptor-binding domain of the SARS-CoV-2 spike protein with ultrapotent neutralizing activity. One such antibody, NE12, neutralizes an early isolate, the WA-1 strain, as well as the Alpha and Delta variants with half-maximal inhibitory concentrations at picomolar level. A second antibody, NA8, has an unusual breadth of neutralization, with picomolar activity against both the Beta and Omicron variants. The prophylactic and therapeutic efficacy of NE12 and NA8 was confirmed in preclinical studies in the golden Syrian hamster model. Analysis by cryo-EM illustrated the structural basis for the neutralization properties of NE12 and NA8. Potent and broadly neutralizing antibodies against conserved regions of the SARS-CoV-2 spike protein may play a key role against future variants of concern that evade immune control.

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