Targeting the rpoB gene using nested PCR-restriction fragment length polymorphism for identification of nontuberculous mycobacteria in hospital tap water

2008 ◽  
Vol 46 (6) ◽  
pp. 608-614 ◽  
Author(s):  
Ji-Hyun Shin ◽  
Hae-Kyung Lee ◽  
Eun-Jin Cho ◽  
Jae-Yon Yu ◽  
Yeon-Ho Kang
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Nested Pcr ◽  
Fragment Length Polymorphism ◽  
Nontuberculous Mycobacteria ◽  
Tap Water ◽  
Rpob Gene ◽  
Restriction Fragment Length

scholarly journals Identification of Nontuberculous Mycobacteria Existing in Tap Water by PCR-Restriction Fragment Length Polymorphism

2002 ◽  
Vol 68 (6) ◽  
pp. 3159-3161 ◽  
Author(s):  
Chiao-tang Chang ◽  
Ling-yu Wang ◽  
Chen-yi Liao ◽  
Shiao-ping Huang
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Nontuberculous Mycobacteria ◽  
Tap Water ◽  
Possible Cause ◽  
Acid Fast Staining ◽  
Restriction Fragment Length

ABSTRACT This paper presents the finding of the possible cause of the high false-positive rate in acid-fast staining in histological examinations. Using acid-fast staining, culture, and PCR, acid-fast bacilli were detected in 83.7% of 49 hospital tap water samples and nontuberculous mycobacteria (NTM) were detected in 20.4% of the same 49 samples. The 10 NTM isolates were also identified to the species level using PCR-restriction fragment length polymorphism. Our findings indicate that NTM in hospital tap water are the possible cause of false positives in acid-fast staining and of nosocomial infection in immunocompromised patients.

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