Promoter exchange of the cryptic nonribosomal peptide synthetase gene for oligopeptide production in Aspergillus oryzae

ABSTRACTWYK-1 is a dipeptidyl peptidase IV inhibitor produced byAspergillus oryzaestrain AO-1. Because WYK-1 is an isoquinoline derivative consisting of threel-amino acids, we hypothesized that a nonribosomal peptide synthetase was involved in its biosynthesis. We identified 28 nonribosomal peptide synthetase genes in the sequenced genome ofA. oryzaeRIB40. These genes were also identified in AO-1. Among them, AO090001000009 (wykN) was specifically expressed under WYK-1-producing conditions in AO-1. Therefore, we constructedwykNgene disruptants of AO-1 after nonhomologous recombination was suppressed by RNA interference to promote homologous recombination. Our results demonstrated that the disruptants did not produce WYK-1. Furthermore, the expression patterns of 10 genes downstream ofwykNwere similar to the expression pattern ofwykNunder several conditions. Additionally, homology searches revealed that some of these genes were predicted to be involved in WYK-1 biosynthesis. Therefore, we propose thatwykNand the 10 genes identified in this study constitute the WYK-1 biosynthetic gene cluster.

Download Full-text

Identification of a novel polyketide synthase–nonribosomal peptide synthetase (PKS–NRPS) gene required for the biosynthesis of cyclopiazonic acid in Aspergillus oryzae

Fungal Genetics and Biology ◽

10.1016/j.fgb.2008.09.006 ◽

2008 ◽

Vol 45 (12) ◽

pp. 1608-1615 ◽

Cited By ~ 63

Author(s):

Masafumi Tokuoka ◽

Yasuyo Seshime ◽

Isao Fujii ◽

Katsuhiko Kitamoto ◽

Tadashi Takahashi ◽

...

Keyword(s):

Aspergillus Oryzae ◽

Polyketide Synthase ◽

Cyclopiazonic Acid ◽

Nonribosomal Peptide Synthetase ◽

Nrps Gene ◽

Nonribosomal Peptide ◽

Peptide Synthetase

Download Full-text

Faculty Opinions recommendation of Investigation of indigoidine synthetase reveals conserved active site base residue of nonribosomal peptide synthetase oxidases.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.738101115.793575536 ◽

2020 ◽

Author(s):

Zixin Deng ◽

Xudong Qu

Keyword(s):

Active Site ◽

Nonribosomal Peptide Synthetase ◽

Nonribosomal Peptide ◽

Peptide Synthetase

Download Full-text

Faculty Opinions recommendation of N-acylation during glidobactin biosynthesis by the tridomain nonribosomal peptide synthetase module GlbF.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.9511956.10161054 ◽

2011 ◽

Author(s):

Jan Grunewald

Keyword(s):

Nonribosomal Peptide Synthetase ◽

Nonribosomal Peptide ◽

Peptide Synthetase

Download Full-text

Draft Genome Sequence of Saccharomonospora sp. Strain LRS4.154, a Moderately Halophilic Actinobacterium with the Biotechnologically Relevant Polyketide Synthase and Nonribosomal Peptide Synthetase Systems

Genome Announcements ◽

10.1128/genomea.00392-17 ◽

2017 ◽

Vol 5 (21) ◽

Author(s):

Scarlett Alonso-Carmona ◽

Blanca Vera-Gargallo ◽

Rafael R. de la Haba ◽

Antonio Ventosa ◽

Horacio Sandoval-Trujillo ◽

...

Keyword(s):

Genome Sequence ◽

Polyketide Synthase ◽

Draft Genome ◽

Nonribosomal Peptide Synthetase ◽

Open Reading Frames ◽

Draft Genome Sequence ◽

Nonribosomal Peptide ◽

Moderately Halophilic ◽

Peptide Synthetase ◽

Reading Frames

ABSTRACT The draft genome sequence of Saccharomonospora sp. strain LRS4.154, a moderately halophilic actinobacterium, has been determined. The genome has 4,860,108 bp, a G+C content of 71.0%, and 4,525 open reading frames (ORFs). The clusters of PKS and NRPS genes, responsible for the biosynthesis of a large number of biomolecules, were identified in the genome.

Download Full-text