Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia australis Hook. f. (Posidoniaceae), with cross-amplification in the sympatric P. sinuosa

2009 ◽  
Vol 1 (1) ◽  
pp. 273-276 ◽  
Author(s):  
E. A. Sinclair ◽  
J. Anthony ◽  
G. T. Coupland ◽  
M. Waycott ◽  
M. D. Barrett ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Cross Amplification ◽  
Polymorphic Microsatellite

scholarly journals Cross-amplification and Characterization of Polymorphic Microsatellite Markers From Acacia (Senegalia) mellifera and Acacia brevispica to Acacia senegal (L.) Willd.

2010 ◽  
Vol 59 (1-6) ◽  
pp. 285-288 ◽  
Author(s):  
Stephen F. Omondi ◽  
O. G. Dangasuk ◽  
D. W. Odee ◽  
S. Cavers ◽  
D. P. Khasa
Keyword(s):  
Microsatellite Markers ◽  
Allelic Diversity ◽  
Breeding Systems ◽  
Acacia Senegal ◽  
Cross Amplification ◽  
Expected Heterozygosity ◽  
Potential Source ◽  
Polymorphic Microsatellite ◽  
Acacia Mellifera

Abstract Seven polymorphic microsatellite markers isolated from Acacia brevispica and Acacia mellifera were successfully cross-amplified in Acacia senegal. The loci were surveyed for polymorphism using 30 samples. Allelic diversity ranged from 4 (Ame02, Ab06 and Ab18) to 13 (Ab26) per locus. The expected heterozygosity (HE) ranged from 0.543 (Ame02) to 0.868 (Ab26) while observed heterozygosity (HO) ranged from 0.516 (Ame05) to 0.800 (Ame03). Cross-amplification of these loci represents a potential source of co-dominant markers and will be useful in the study of genetic diversity, structure, gene flow and breeding systems of this important Acacia species.

scholarly journals Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus)

2020 ◽  
Author(s):  
Meaghan A Swintek ◽  
Ryan P Walter
Keyword(s):  
Genetic Variation ◽  
Microsatellite Markers ◽  
Shark Species ◽  
Bonferroni Correction ◽  
Cross Amplification ◽  
Population Analyses ◽  
Polymorphic Microsatellite ◽  
Somniosus Microcephalus ◽  
Successful Amplification

Abstract Objective The objectives of this research are to isolate, develop and characterize polymorphic microsatellite markers for use in Greenland sharks ( Somniosus microcephalus ). Despite utility in population analyses, microsatellite markers have not been previously developed for this species. Development of these markers, and successful amplification in closely related Pacific sleeper sharks ( S. pacificus ), will facilitate research in the genetic variation of contemporary and future populations of sleeper shark species. Results Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 32 S. microcephalus individuals from Grise Fjord (n = 16) and Svalbard (n = 20). Each locus yielded between 2 to 9 alleles and observed heterozygosity ranged from 0.11 to 0.70 when estimated across both sites. One locus and three loci deviated from HWE following Bonferroni correction, for individuals sampled from Grise Fjord and Svalbard, respectively. Cross-amplification was successful at every locus for five of the ten S. pacificus individuals.

Isolation and cross-amplification of the first set of polymorphic microsatellite markers of two high-Andean cushion plants

2018 ◽  
Vol 97 (S1) ◽  
pp. 95-100
Author(s):  
Ian S. Acuña-Rodríguez ◽  
Nicolas Gouin ◽  
Leonardo Cifuentes-Lisboa ◽  
Francisco A. Squeo
Keyword(s):  
Microsatellite Markers ◽  
Cross Amplification ◽  
Cushion Plants ◽  
Polymorphic Microsatellite

Characterization of polymorphic microsatellite markers in Valencia letourneuxi (Valenciidae) and cross-amplification in two other cyprinodontiform species

2009 ◽  
Vol 1 (1) ◽  
pp. 27-30 ◽  
Author(s):  
Emmanouella Vogiatzi ◽  
Eleni Kalogianni ◽  
Sofia Giakoumi ◽  
Antonios Magoulas ◽  
Costas S. Tsigenopoulos
Keyword(s):  
Microsatellite Markers ◽  
Cross Amplification ◽  
Polymorphic Microsatellite
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