Development and characterization of 20 polymorphic microsatellite markers for the Texas hornshell, Popenaias popeii (Bivalvia: Unionidae), through next-generation sequencing

This study developed and characterized 68 novel polymorphic microsatellite markers from black Amur bream Megalobrama terminalis by next-generation sequencing. Variability was tested on 36 individuals collected from Qiantang River, Zhejiang Province, China. The number of alleles per locus ranged from 2 to 13. Observed heterozygosity ranged from 0.028 to 0.944, whereas the expected heterozygosity ranged from 0.028 to 0.887. Polymorphism Information Content ranged from 0.027 to 0.862. Moreover, 53 microsatellites were in agreement with Hardy–Weinberg equilibrium. Twenty-eight pairwise tests in 33 microsatellite loci indicated linkage disequilibrium. These microsatellites are a valuable tool for further genetics studies of this species.

Download Full-text

Development and characterization of 19 novel microsatellite markers in the Pacific seaweed pipefish Syngnathus schlegeli using next-generation sequencing

Molecular Biology Reports ◽

10.1007/s11033-018-4396-0 ◽

2018 ◽

Vol 45 (6) ◽

pp. 2831-2834 ◽

Cited By ~ 1

Author(s):

Takehiro Endo ◽

Masashi Sekino ◽

Atushi Fujiwara ◽

Atsushi Sogabe

Keyword(s):

Next Generation Sequencing ◽

Microsatellite Markers ◽

Next Generation ◽

The Pacific ◽

Generation Sequencing

Download Full-text

Microsatellite development via next-generation sequencing in Acacia stenophylla (Fabaceae) and Duma florulenta (Polygonaceae): two ecologically important plant species of Australian dryland floodplains

10.7287/peerj.preprints.27027v1 ◽

2018 ◽

Author(s):

Bruce F Murray ◽

Michael A Reid ◽

Shu-Biao Wu

Keyword(s):

Gene Flow ◽

Next Generation Sequencing ◽

Microsatellite Markers ◽

Information Content ◽

Polymorphic Information Content ◽

Extreme Variability ◽

Polymorphic Microsatellite ◽

Generation Sequencing ◽

Semi Arid

Duma florulenta and Acacia stenophylla are two ecologically important but understudied species that naturally occur on the floodplains and riverbanks of Australia’s arid and semi-arid river systems. This paper describes the discovery and characterization of 12 and 13 polymorphic microsatellite markers for D. florulenta and A. stenophylla respectively. The number of alleles per locus for D. florulenta ranged from 2-12 with an average of 6.1. Across all samples, observed and expected heterozygosities ranged from 0.026 to 0.784 and 0.026 to 0.824 respectively and mean polymorphic information content was equal to 0.453. For A. stenophylla, the number of alleles per locus ranged between 2 and 8 with an overall mean of 4.8. Across all samples, observed and expected heterozygosities ranged from 0.029 to 0.650 and 0.029 to 0.761 respectively and mean polymorphic information content was 0.388. The developed suites of 12 and 13 microsatellite markers for D. florulenta and A. stenophylla respectively provide opportunity for novel research into mechanisms of gene flow, dispersal and breeding system and how they operate under the extreme variability these species are exposed to in the environments in which they live.

Download Full-text