Development of quantitative PCR primers and probes for environmental DNA detection of amphibians in Ontario

2018 ◽  
Vol 11 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Kaela Beauclerc ◽  
Kristyne Wozney ◽  
Caleigh Smith ◽  
Chris Wilson
Keyword(s):  
Quantitative Pcr ◽  
Dna Detection ◽  
Environmental Dna ◽  
Pcr Primers

scholarly journals Effects of preservation strategies on environmental DNA detection and quantification using ddPCR

2021 ◽  
Author(s):  
Quentin Mauvisseau ◽  
David Halfmaerten ◽  
Sabrina Neyrinck ◽  
Alfred Burian ◽  
Rein Brys
Keyword(s):  
Dna Detection ◽  
Environmental Dna ◽  
Detection And Quantification

Trans-Oral Brush Biopsies and Quantitative PCR for EBV DNA Detection and Screening of Nasopharyngeal Carcinoma

2014 ◽  
Vol 150 (4) ◽  
pp. 602-609 ◽  
Author(s):  
Raymond H. W. Ng ◽  
Roger Ngan ◽  
William Ignace Wei ◽  
Patrick J. Gullane ◽  
John Phillips
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Quantitative Pcr ◽  
Dna Detection ◽  
Ebv Dna

scholarly journals Development of a quantitative PCR assay for detection of redside shiner (Richardsonius balteatus) from environmental DNA

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Marshal S. Hoy ◽  
Carl O. Ostberg
Keyword(s):  
Quantitative Pcr ◽  
Water Samples ◽  
Effective Means ◽  
Environmental Dna ◽  
Sympatric Species ◽  
Negative Control ◽  
Control Site ◽  
Qpcr Assay ◽  
Redside Shiner ◽  
Richardsonius Balteatus

Abstract Objective A quantitative PCR (qPCR) assay for the detection of redside shiner (Richardsonius balteatus) environmental DNA (eDNA) was designed as a side product of a larger project aimed at using eDNA to determine the presence and geographic extent of native and non-native fishes in the reservoirs and associated tributaries above the three mainstem dams (Ross, Diablo, Gorge) on the Skagit River, Washington, USA. The eDNA survey results can be used to help guide additional sampling efforts that include traditional sampling methods, such as electrofishing and netting. Results The redside shiner qPCR assay (RSSCOI_540-601) was validated by testing for sensitivity using redside shiner genomic DNA from three different populations and by testing for specificity against 30 potentially sympatric species. No non-target amplification was observed in our validation tests. We then evaluated the assay on field-collected water samples where there are known populations of redside shiner and a negative control site where the target species is known to be absent. The field-collected water samples tested positive at the redside shiner sites and tested negative at the negative control site. The assay could provide resource managers with an effective means for surveying and monitoring redside shiner populations.

scholarly journals Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis

2016 ◽  
Vol 194 ◽  
pp. 209-216 ◽  
Author(s):  
Taylor M. Wilcox ◽  
Kevin S. McKelvey ◽  
Michael K. Young ◽  
Adam J. Sepulveda ◽  
Bradley B. Shepard ◽  
...  
Keyword(s):  
Salvelinus Fontinalis ◽  
Dna Detection ◽  
Environmental Dna ◽  
Detection Probabilities

scholarly journals Water Flow and Biofilm Cover Influence Environmental DNA Detection in Recirculating Streams

2018 ◽  
Vol 52 (15) ◽  
pp. 8530-8537 ◽  
Author(s):  
Arial J. Shogren ◽  
Jennifer L. Tank ◽  
Scott P. Egan ◽  
Olivia August ◽  
Emma J. Rosi ◽  
...  
Keyword(s):  
Water Flow ◽  
Dna Detection ◽  
Environmental Dna

scholarly journals Seasonal variation in environmental DNA detection in sediment and water samples

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0191737 ◽  
Author(s):  
Andrew S. Buxton ◽  
Jim J. Groombridge ◽  
Richard A. Griffiths
Keyword(s):  
Seasonal Variation ◽  
Water Samples ◽  
Dna Detection ◽  
Environmental Dna
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