Isolation and characterization of 34 SNP markers in Hucho bleekeri

Abstract The black rockfish (Sebastes schlegelii) is an economically important species. However, wild S. schlegelii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of S. schlegelii. In this study, 38 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1400 to 0.6400 and 0.1487 to 0.4978, respectively. The minor allele frequency raged from 0.1429 to 0.4694. Polymorphic information content ranged from 0.174 to 0.365. Four SNPs were found to be deviated significantly from the HWE (P<0.05). These SNP markers will serve as a useful tool for genetic studies and population evaluation aimed at the conservation of S. schlegelii.

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Isolation and characterization of gene-derived single nucleotide polymorphism (SNP) markers in Scylla paramamosain

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2011.05.024 ◽

2011 ◽

Vol 39 (4-6) ◽

pp. 419-424 ◽

Cited By ~ 8

Author(s):

Hongyu Ma ◽

Qunqun Ma ◽

Chunyan Ma ◽

Lingbo Ma

Keyword(s):

Single Nucleotide Polymorphism ◽

Snp Markers ◽

Scylla Paramamosain ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Isolation And Characterization

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Isolation and characterization of 50 SNP markers in Triplophysa siluroides

Conservation Genetics Resources ◽

10.1007/s12686-018-1059-3 ◽

2018 ◽

Vol 12 (1) ◽

pp. 25-28

Author(s):

Yeyu Chen ◽

Quan Gong ◽

Ya Liu ◽

Mingjiang Song ◽

Jun Du ◽

...

Keyword(s):

Snp Markers ◽

Isolation And Characterization

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Isolation And Characterization Of 45 SNP Markers In Triplophysa Tenuis

10.21203/rs.3.rs-551660/v1 ◽

2021 ◽

Author(s):

Ya Liu ◽

Yeyu Chen ◽

Jiansheng Lai ◽

Hongyu Ke ◽

Zhongmeng Zhao ◽

...

Keyword(s):

Polymorphic Information Content ◽

Snp Markers ◽

Gansu Province ◽

The Novel ◽

Genetic Management ◽

Polymorphic Snps ◽

Isolation And Characterization ◽

Population Conservation ◽

Hardy Weinberg Equilibrium

Abstract Triplophysa tenuis is an endemic species to China, which mainly distributed in Xinjiang and Gansu province. Effective conservation and management of this species is limited by insufficient molecular markers. In the present study, we reported the isolation and characterization of 45 SNP markers in T. tenuis. The minor allele frequency ranged from 0.046 to 0.500, and the observed and expected heterozygosities ranged from 0.061 to 0.667 and 0.088 to 0.508, respectively. Polymorphic information content ranged from 0.083 to 0.375. Among these SNPs, three loci showed significant departures from the Hardy–Weinberg equilibrium. The novel polymorphic SNPs will be helpful for the future study on genetic management and population conservation for this species.

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Development and Characterization of 135 SNP Markers in Chiton Acanthochitona Rubrolineatus

10.21203/rs.3.rs-687709/v1 ◽

2021 ◽

Author(s):

Jirui zhang ◽

Yunqi Song ◽

Chenghong Wang ◽

Shukui Zhang ◽

Zilin Jiang ◽

...

Keyword(s):

Snp Markers ◽

Wild Populations ◽

Effective Protection ◽

Population Genetic Analysis ◽

Important Species ◽

Polymorphic Snps ◽

Isolation And Characterization ◽

Expected Heterozygosity ◽

Hardy Weinberg Equilibrium

Abstract Acanthochitona rubrolineatus (Lischke, 1873) (Polyplacophora, Neoloricata, Cryptoplacidae) is an important species widely distributed in the middle and low tide zone of coastal intertidal zone along the China Sea coast. In recent years, the serious pollution of seawater has caused a sharp decline in the number of wild populations of A. rubrolineatus. Lacking of effective molecular markers limits the effective protection and management of this species. Studies for the isolation and characterization of 135 A. rubrolineatus SNPs markers were carried out. The frequency of minor allele ranges from 0.0125 to 0.5000. The observed heterozygosity and expected heterozygosity are 0.0000-0.9750, 0.0731-0.6696, respectively. The inbreeding value varies from -0.2902 to 0.9966. Among them, there are 27 sites markablely differently from Hardy-Weinberg equilibrium (P < 0.05). The study of the polymorphic SNPs will provide a therotical basis for further analysis of population genetic analysis on A. rubrolineatus.

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