In vitro plant regeneration of non-toxic Jatropha curcas L.: Direct shoot organogenesis from cotyledonary petiole explants

2010 ◽  
Vol 13 (3) ◽  
pp. 189-194 ◽  
Author(s):  
Nitish Kumar ◽  
K. G. Vijay Anand ◽  
Muppala P. Reddy
Keyword(s):  
Plant Regeneration ◽  
Jatropha Curcas ◽  
Shoot Organogenesis ◽  
In Vitro Plant Regeneration ◽  
Petiole Explants ◽  
Jatropha Curcas L ◽  
Cotyledonary Petiole ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

scholarly journals In Vitro Plant Regeneration of Sweetpotato Through Direct Shoot Organogenesis

2018 ◽  
Vol 06 (01) ◽  
Author(s):  
Nzaro G Makenzi ◽  
Wilton M Mbinda ◽  
Richard Oduor Okoth ◽  
Mathews Piero Ngugi
Keyword(s):  
Plant Regeneration ◽  
Shoot Organogenesis ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot

scholarly journals Efficient culture protocol for plant regeneration from cotyledonary petiole explants of Jatropha curcas L.

2016 ◽  
Vol 30 (5) ◽  
pp. 907-914 ◽  
Author(s):  
Ying Liu ◽  
Jiannong Lu ◽  
Hongbo Zhu ◽  
Linfeng Li ◽  
Yuzhen Shi ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Jatropha Curcas ◽  
Petiole Explants ◽  
Jatropha Curcas L ◽  
Cotyledonary Petiole ◽  
Culture Protocol

scholarly journals Direct Shoot Organogenesis of Medicago sativa

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 628f-628
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass
Keyword(s):  
Genetic Transformation ◽  
Shoot Regeneration ◽  
Shoot Organogenesis ◽  
Multiple Shoots ◽  
Direct Shoot Regeneration ◽  
Callus Initiation ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot ◽  
In Vitro Shoot Regeneration

An efficient and reliable protocol of in vitro shoot regeneration must be first established to have a successful genetic transformation. As a member of legume family, alfalfa is very difficult for direct shoot regeneration. There is no published information on direct shoot organogenesis, although success has been well documented on embryogenesis, which must go through callus stage. Different plant growth regulators at various concentrations were evaluated for callus initiation, development, and direct shoot regeneration. Multiple shoots were produced directly from each individual explant. This will provide an efficient means for production of transgenic alfalfa plants. Therefore, genetic transformation of Medicago germplasm will be significantly expedited.

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