scholarly journals Culture-independent molecular analysis of bacterial diversity in uranium-ore/-mine waste-contaminated and non-contaminated sites from uranium mines

3 Biotech ◽  
2011 ◽  
Vol 1 (4) ◽  
pp. 261-272 ◽  
Author(s):  
Paltu Kumar Dhal ◽  
Ekramul Islam ◽  
Sufia K. Kazy ◽  
Pinaki Sar
Keyword(s):  
Bacterial Diversity ◽  
Molecular Analysis ◽  
Mine Waste ◽  
Contaminated Sites ◽  
Uranium Ore ◽  
Uranium Mines ◽  
Culture Independent

Minimal and dynamic bacterial diversity in grease trap revealed by molecular analysis

2012 ◽  
Vol 43 (1) ◽  
pp. 29-41 ◽  
Author(s):  
Lucas F.S. ◽  
Richard F. ◽  
Adolphe Y. ◽  
Haenn S. ◽  
Moilleron R. ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Molecular Analysis

Concept for Disposal of Class 2 and Class 3 Radioactive Waste in Underground Workings with Isolating Backfilling using Paste made with Processed Uranium Ore Materials

2020 ◽  
pp. 31-36
Author(s):  
E.V. Kuzmin ◽  
A.V. Kalakutskiy ◽  
M.A. Tarasov ◽  
A.A. Morozov
Keyword(s):  
Radioactive Waste ◽  
Host Rock ◽  
Deep Levels ◽  
Uranium Ore ◽  
Waste Storage ◽  
Uranium Mines ◽  
Underground Workings ◽  
Industrial Mining ◽  
Space Data ◽  
Radioactive Waste Storage

The paper presents a concept for disposal of Class 2 and Class 3 radioactive waste in existing workings and chambers in the underground uranium mines of the Priargunsky Industrial Mining and Chemical Union PJSC with isolation of radioactive waste storage chambers using paste backfilling made with processed uranium ore materials and sealing of cracks in host rock by injection consolidation. The need is shown to enhance the strength of radioactive waste containers to increase the number of tiers in the storage blocks to make better use of the available chamber space. Data is provided on the forecast hydrogeological conditions in deep levels (up to 1000 m) of Mine No.6.

Culture independent bacterial diversity of Changme Khang and Changme Khangpu glaciers of North Sikkim, India

2019 ◽  
Vol 2 (3) ◽  
pp. 241-253 ◽  
Author(s):  
Mingma Thundu Sherpa ◽  
Ishfaq Nabi Najar ◽  
Sayak Das ◽  
Nagendra Thakur
Keyword(s):  
Bacterial Diversity ◽  
Culture Independent

Molecular bacterial diversity and bioburden of commercial airliner cabin air

2007 ◽  
Vol 53 (11) ◽  
pp. 1259-1271 ◽  
Author(s):  
Myron T. La Duc ◽  
Tara Stuecker ◽  
Kasthuri Venkateswaran
Keyword(s):  
Bacterial Diversity ◽  
Rrna Gene ◽  
Human Respiratory Tract ◽  
Atp Assay ◽  
Gram Positive Bacteria ◽  
Air Impingement ◽  
Culture Independent ◽  
Bacterial Enumeration ◽  
Gradual Accumulation ◽  
Opportunistic Pathogenic

Culture-independent, biomarker-targeted bacterial enumeration and identification strategies were employed to estimate total bacterial burden and diversity within the cabin air of commercial airliners. Samples from each of 4 flights on 2 commercial carriers were collected via air-impingement. The total viable microbial population ranged from below detection limits to 4.1 × 106cells/m3of air, as assessed by the ATP assay. A gradual accumulation of microbes was observed from the time of passenger boarding through mid-flight, followed by a sharp decline in bacterial abundance and viability from the initiation of descent through landing. Representatives of the α-, β-, and γ-Proteobacteria, as well as Gram-positive bacteria, were isolated in varying abundance. Neisseria meningitidis rRNA gene sequences were retrieved in great abundance from Airline A followed by Streptococcus oralis/mitis sequences. Pseudomonas synxantha sequences dominated Airline B clone libraries, followed by those of N. meningitidis and S. oralis/mitis. The cabin air samples examined herein housed low bacterial diversity and were often dominated by a particular subset of bacteria: opportunistic pathogenic inhabitants of the human respiratory tract and oral cavity.

Bacterial diversity and real-time PCR based assessment oflinAandlinBgene distribution at hexachlorocyclohexane contaminated sites

2013 ◽  
Vol 55 (3) ◽  
pp. 363-373 ◽  
Author(s):  
Devi Lal ◽  
Swati Jindal ◽  
Hansi Kumari ◽  
Simran Jit ◽  
Aeshna Nigam ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Real Time ◽  
Real Time Pcr ◽  
Contaminated Sites

scholarly journals Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity

1998 ◽  
Vol 180 (24) ◽  
pp. 6793-6793 ◽  
Author(s):  
Philip Hugenholtz ◽  
Brett M. Goebel ◽  
Norman R. Pace
Keyword(s):  
Bacterial Diversity ◽  
Culture Independent

scholarly journals Method for Collecting Air-Water Interface Microbes Suitable for Subsequent Microscopy and Molecular Analysis in both Research and Teaching Laboratories

2004 ◽  
Vol 70 (4) ◽  
pp. 2486-2493 ◽  
Author(s):  
Margaret C. Henk
Keyword(s):  
Molecular Analysis ◽  
Bulk Water ◽  
Water Interface ◽  
Sampling Device ◽  
Force Microscopy ◽  
Research And Teaching ◽  
Transmission Electron ◽  
Air Water Interface ◽  
Culture Independent ◽  
Fluorescence Light

ABSTRACT A method has been developed for collecting air-water interface (AWI) microbes and biofilms that enables analysis of the same sample with various combinations of bright-field and fluorescence light microscopy optics, scanning and transmission electron microscopy (TEM), and atomic force microscopy. The identical sample is then subjected to molecular analysis. The sampling tool consists of a microscope slide supporting appropriate substrates, TEM grids, for example, that are removable for the desired protocols. The slide with its substrates is then coated with a collodion polymer membrane to which in situ AWI organisms adhere upon contact. This sampling device effectively separates the captured AWI bacterial community from the bulk water community immediately subtending. Preliminary data indicate that the AWI community differs significantly from the water column community from the same sample site when both are evaluated with microscopy and with 16S ribosomal DNA sequence-based culture-independent comparisons. This microbe collection method can be used at many levels in research and teaching.

scholarly journals Culture independent molecular analysis of bacterial communities in the mangrove sediment of Sundarban, India

2010 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Abhrajyoti Ghosh ◽  
Nirmalya Dey ◽  
Amit Bera ◽  
Amit Tiwari ◽  
KB Sathyaniranjan ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Bacterial Communities ◽  
Mangrove Sediment ◽  
Culture Independent

scholarly journals Molecular analysis of bacterial diversity in kerosene-based drilling fluid from the deep ice borehole at Vostok, East Antarctica

2007 ◽  
Vol 59 (2) ◽  
pp. 289-299 ◽  
Author(s):  
Irina A. Alekhina ◽  
Dominique Marie ◽  
Jean Robert Petit ◽  
Valery V. Lukin ◽  
Vladimir M. Zubkov ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Molecular Analysis ◽  
Drilling Fluid ◽  
East Antarctica
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