scholarly journals A novel in vitro transformation of Lepidium draba L. using rapid direct shoot regeneration

3 Biotech ◽  
2017 ◽  
Vol 7 (5) ◽  
Author(s):  
Zahra Zinhari ◽  
Shahram Pourseyedi ◽  
Jefar Zolalo
Keyword(s):  
Shoot Regeneration ◽  
Direct Shoot Regeneration ◽  
In Vitro Transformation ◽  
Lepidium Draba ◽  
Direct Shoot

scholarly journals Improvement of micropropagation protocol of Phalaenopsis sp. for the method of direct shoot regeneration from nodes of floral spikes

2012 ◽  
pp. 141-150
Author(s):  
Marija Markovic ◽  
Milos Tanasic ◽  
Nevena Stojic ◽  
Radivoje Bulatovic ◽  
Marta Jovic ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Coconut Water ◽  
Soy Flour ◽  
Multiplication Rate ◽  
Direct Shoot Regeneration ◽  
Medium Components ◽  
Cultivation In Vitro ◽  
Direct Shoot

This paper succesfully investigated the possibility of modification of the micropropagation protocol of Phalaenopsis sp. with an aim to simplify the procedure and reduce the costs. The obtained results show that some medium components can be succesfully omitted (coconut water, glutamine, 2-morpholinoethanesulfonic acid) and some of them (peptone) can be replaced with a cheaper constituent (soy flour) while preserving the quality of the obtained microplants. The multiplication rate was 7,6 shoots per explant after the period of 150 days of cultivation in vitro. On the same medium 60% of explants were rooted and roots were mostly well developed.

scholarly journals Direct Shoot Organogenesis of Medicago sativa

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 628f-628
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass
Keyword(s):  
Genetic Transformation ◽  
Shoot Regeneration ◽  
Shoot Organogenesis ◽  
Multiple Shoots ◽  
Direct Shoot Regeneration ◽  
Callus Initiation ◽  
Direct Shoot Organogenesis ◽  
Direct Shoot ◽  
In Vitro Shoot Regeneration

An efficient and reliable protocol of in vitro shoot regeneration must be first established to have a successful genetic transformation. As a member of legume family, alfalfa is very difficult for direct shoot regeneration. There is no published information on direct shoot organogenesis, although success has been well documented on embryogenesis, which must go through callus stage. Different plant growth regulators at various concentrations were evaluated for callus initiation, development, and direct shoot regeneration. Multiple shoots were produced directly from each individual explant. This will provide an efficient means for production of transgenic alfalfa plants. Therefore, genetic transformation of Medicago germplasm will be significantly expedited.

Ethylene and CO2 affect direct shoot regeneration from the petiolar ends of Paulownia kawakamii leaves cultured in vitro

1996 ◽  
Vol 20 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Prakash P. Kumar ◽  
C. Dimps Rao ◽  
Chong-Jin Goh
Keyword(s):  
Shoot Regeneration ◽  
Direct Shoot Regeneration ◽  
Direct Shoot

scholarly journals High frequency direct shoot regeneration from Kazakh commercial potato cultivars

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9447
Author(s):  
Laura S. Abeuova ◽  
Balnur R. Kali ◽  
Aizhan O. Rakhimzhanova ◽  
Sara S. Bekkuzhina ◽  
Shuga A. Manabayeva
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Potato Cultivars ◽  
Direct Regeneration ◽  
Naphthaleneacetic Acid ◽  
Direct Shoot Regeneration ◽  
Potato Genome ◽  
Direct Shoot

Potato (Solanum tuberosum L.) is the third most economically important crop in the world and has a high nutritional value. In this study, the in vitro culture response of four widely grown in Kazakhstan potato cultivars, Astanalyk, Monument Kunaev, Tokhtar, and Aksor, was investigated using stem and leaf explants. Published protocols were evaluated and optimized to develop a more efficient protocol for the regeneration of plants from local potato cultivars in tissue culture, which is a prerequisite to facilitate potato genome modification. The explants were cultured on solid Murashige and Skoog medium supplemented with different concentrations and combinations of zeatin, 6-benzylaminopurine (BAP), gibberellic acid (GA3), 1-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA). The maximum regeneration was induced from the stem internodal explants. A significant effect of the explant source on direct regeneration was confirmed with statistical analysis. The number of shoots obtained from the internode was 10.0 from cv. Aksor followed by cvs. Tokhtar and Astanalyk. The medium DRM-VIII with 1 mg/l zeatin, 0.1 mg/l IAA and 7.0 mg/l GA3 was considered the best for direct shoot regeneration and multiple shoot formation from all cultivars. To conclude, we outline a protocol for direct plant regeneration from four potato cultivars. Our findings suggest commercial cultivars Astanalyk and Aksor are good candidates for developing the genome-edited plants through direct shoot regeneration.

scholarly journals Regeneration of Paeonia mlokosewitschii Lom. and P. tenuifolia L. in vitro from different explants

2014 ◽  
Vol 67 (3-4) ◽  
pp. 223-227 ◽  
Author(s):  
Teresa Orlikowska ◽  
Agnieszka Marasek ◽  
Danuta Kucharska
Keyword(s):  
Shoot Regeneration ◽  
Direct Shoot Regeneration ◽  
In Vitro Shoots ◽  
Leaf Veins ◽  
Nodular Callus ◽  
Spherical Structures ◽  
Chemical Conditions ◽  
Direct Shoot

The pattern of regeneration from tissues of <em>Paeonia mlokosewitschii</em> and <em>P. tenuifolia</em> cultured in vitro in the same chemical conditions depended on the initial explant. Direct shoot regeneration was obtained from the bases of petioles and petals, and leaf veins. Vegetative initial buds and regenerated in vitro shoots produced on their bases slowly growing nodular callus which was very productive in repetitive shoot regeneration. The tops of stems, flower bases, sepals, petals and ovary walls produced small callus which regenerated white and red spherical structures within 1.5 years. After that time also from those cultures arised nodular, shoot regenerating callus developed.

In vitro studies on direct shoot regeneration of Stevia rebaudiana using nodal explants

Crop Research ◽  
2017 ◽  
Vol 52 (6) ◽  
pp. 259
Author(s):  
Puneet Beniwal ◽  
Priyanka Siwach ◽  
Vijay S. Beniwal ◽  
Vikas Hooda
Keyword(s):  
Shoot Regeneration ◽  
Stevia Rebaudiana ◽  
In Vitro Studies ◽  
Nodal Explants ◽  
Direct Shoot Regeneration ◽  
Direct Shoot
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