scholarly journals Correction to: Development of allele-specific loop-mediated isothermal amplification (AS-LAMP) to detect the tebufenozide-resistant allele in the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae)

2021 ◽  
Author(s):  
Miwa Uchibori-Asano ◽  
Toru Uchiyama ◽  
Akiya Jouraku ◽  
Tetsuro Shinoda
Keyword(s):  
Isothermal Amplification ◽  
Resistant Allele ◽  
Loop Mediated Isothermal Amplification ◽  
Allele Specific ◽  
Adoxophyes Honmai

scholarly journals Detection of G119S ace-1 R mutation in field-collected Anopheles gambiae mosquitoes using allele-specific loop-mediated isothermal amplification (AS-LAMP) method

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Athanase Badolo ◽  
Hironori Bando ◽  
Alphonse Traoré ◽  
Mami Ko-ketsu ◽  
Wamdaogo Moussa Guelbeogo ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Loop Mediated Isothermal Amplification ◽  
Allele Specific

scholarly journals Rapid Detection of Circulating Tumour DNA using Allele Specific Mini-Loop Mediated Isothermal Amplification

2022 ◽  
Author(s):  
Rebecca Allsopp ◽  
Georgios Alexandrou ◽  
Christofer Toumazou ◽  
Simak Ali ◽  
Charles Coombes ◽  
...  
Keyword(s):  
Competitive Inhibition ◽  
Limit Of Detection ◽  
Cost Effective ◽  
Isothermal Amplification ◽  
Loop Formation ◽  
Loop Mediated Isothermal Amplification ◽  
Specific Allele ◽  
Allele Specific ◽  
Specific Reactions ◽  
Circulating Tumour Dna

Abstract Isothermal amplification is an emerging approach for non-invasive, rapid and cost-effective real-time monitoring of cancer specific mutations through circulating tumour DNA (ctDNA). This study demonstrates a compact allele specific (AS) loop mediated isothermal amplification (LAMP) strategy, termed ‘AS-Mini-LAMP’, modelled using wild type (WT) and mutation specific reactions targeting the estrogen receptor ESR1 c.1138G>C (p.E380Q) missense mutation. Allele selectivity, encoded at the 5’-end of the forward and backward inner primers (FIP and BIP) promotes enhanced selectivity upon self-hybridisation, loop formation and self-primed exponential amplification. Inclusion of unmodified self-stabilising (USS) primers aimed to reduce the likelihood of non-specific allele amplification through competitive inhibition and to enhance reaction velocity through an assisted strand displacement ‘swarm’ priming effect. The two assays were optimised using short synthetic WT and E380Q mutant DNA templates, and subsequently validated to a limit of detection of 500 mutant copies in under 25 minutes in ddPCR-confirmed positive (20.7% variant allele frequency) and negative patient plasma cfDNA samples. These results demonstrate the ability of AS-Mini-LAMP to achieve sensitive and selective amplification of actionable mutations present within plasma ctDNA.

In-Silico Automated Allele-Specific Primer Design for Loop-Mediated Isothermal Amplification

2020 ◽  
Author(s):  
George Alexandrou ◽  
Jesus Rodriguez-Manzano ◽  
Kenny Malpartida-Cardenas ◽  
Pantelis Georgiou ◽  
Chris Toumazou ◽  
...  
Keyword(s):  
In Silico ◽  
Primer Design ◽  
Isothermal Amplification ◽  
Specific Primer ◽  
Loop Mediated Isothermal Amplification ◽  
Allele Specific
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