Detection of the papaya strain of papaya ringspot virus (PRSV-P) in Paraguay

The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya.

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Screening the Watermelon Germplasm Collection for Resistance to Papaya Ringspot Virus Type‐W

Crop Science ◽

10.2135/cropsci2002.1324 ◽

2002 ◽

Vol 42 (4) ◽

pp. 1324-1330 ◽

Cited By ~ 18

Author(s):

E. Bruton Strange ◽

Nihat Guner ◽

Zvezdana Pesic‐VanEsbroeck ◽

Todd C. Wehner

Keyword(s):

Virus Type ◽

Germplasm Collection ◽

Ringspot Virus ◽

Papaya Ringspot Virus

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Erratum to: Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other isolates

Virus Genes ◽

10.1007/s11262-007-0099-7 ◽

2007 ◽

Vol 35 (2) ◽

pp. 431-431

Author(s):

Juan Carlos Noa-Carrazana ◽

Diego González-de-León ◽

Laura Silva-Rosales

Keyword(s):

Molecular Characterization ◽

Ringspot Virus ◽

Papaya Ringspot Virus ◽

Severe Isolate

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Transgene-specific and event-specific molecular markers for characterization of transgenic papaya lines resistant to Papaya ringspot virus

Transgenic Research ◽

10.1007/s11248-009-9287-7 ◽

2009 ◽

Vol 18 (6) ◽

pp. 971-986 ◽

Cited By ~ 18

Author(s):

Ming-Jen Fan ◽

Shu Chen ◽

Yi-Jung Kung ◽

Ying-Huey Cheng ◽

Huey-Jiunn Bau ◽

...

Keyword(s):

Molecular Markers ◽

Ringspot Virus ◽

Papaya Ringspot Virus ◽

Transgenic Papaya

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Generation of mild recombinants of papaya ringspot virus to minimize the problem of strain-specific cross protection

Phytopathology ◽

10.1094/phyto-06-21-0272-r ◽

2021 ◽

Author(s):

Thi-Thu-Yen Tran ◽

Tzu-Tung Lin ◽

Chung-Ping Chang ◽

Chun-Hung Chen ◽

Van-Hoa Nguyen ◽

...

Keyword(s):

Nitrous Acid ◽

Carica Papaya ◽

Genomic Sequence ◽

Cross Protection ◽

Ringspot Virus ◽

Limiting Factor ◽

Papaya Ringspot Virus ◽

Severe Damage ◽

Mild Strain ◽

Geographic Regions

Papaya ringspot virus (PRSV) causes severe damage to papaya (Carica papaya L.) and is the primary limiting factor for papaya production worldwide. A nitrous acid-induced mild strain PRSV HA 5-1, derived from Hawaii strain HA, has been applied to control PRSV by cross protection for decades. However, the problem of strain-specific protection hampers its application in Taiwan and other geographic regions outside Hawaii. Here, sequence comparison of the genomic sequence of HA 5-1 with that of HA revealed 69 nucleotide changes, resulting in 31 aa changes in which 16 aa are structurally different. The multiple mutations of HA 5-1 are considered resulting from nitrous-acid induction since 86% of nucleotide changes are transition mutations. The stable HA 5-1 was used as a backbone to generate recombinants carrying individual 3’ fragments of Vietnam severe strain TG5, including NIa, NIb, and CP3’ regions, individually or in combination. Our results indicated that the best heterologous fragment for the recombinant is the region of CP3’, with which symptom attenuation of the recombinant is like that of HA 5-1. This mild recombinant HA51/TG5-CP3’ retained high levels of protection against the homologous HA in papaya plants and significantly increased the protection against the heterologous TG-5. Similarly, HA 5-1 recombinants carrying individual CP3’ fragments from Thailand SMK, Taiwan YK, and Vietnam ST2 severe strains also significantly increase the protection against the corresponding heterologous strains in papaya plants. Thus, our recombinant approach for mild strain generation is a fast and effective way to minimize the problem of strain-specific protection.

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Danos causados pelo Zucchini lethal chlorosis virus (ZLCV) sobre a produção de frutos comerciais de abobrinha de moita 'Caserta'

Summa Phytopathologica ◽

10.1590/s0100-54052009000300011 ◽

2009 ◽

Vol 35 (3) ◽

pp. 223-225 ◽

Cited By ~ 7

Author(s):

José Segundo Giampan ◽

Jorge Alberto Marques Rezende ◽

Sônia Maria De Stefano Piedade

Keyword(s):

Mosaic Virus ◽

Virus Type ◽

Zucchini Yellow Mosaic Virus ◽

Ringspot Virus ◽

Yellow Mosaic Virus ◽

Papaya Ringspot Virus

O ZLCV é um tospovírus encontrado com freqüência causando severos danos em cucurbitáceas. Nesse trabalho avaliaram-se os danos causados pelo ZLCV em abobrinha de moita 'Caserta', em campo na ESALQ/USP, Piracicaba-SP, onde esse vírus é freqüente. Plantas obtidas pela semeadura direta foram monitoradas periodicamente quanto à infecção pelo ZLCV por meio dos sintomas e por PTA-ELISA. Monitorou-se ainda a contaminação com Papaya ringspot virus - type W e Zucchini yellow mosaic virus, desconsiderando a produção dessas plantas. As plantas foram agrupadas em função da época de aparecimento dos sintomas do ZLCV, avaliando a produção de frutos comerciais (FC) e não comerciais (FNC) de cada grupo e comparando com a de plantas que permaneceram sem sintomas até o final do experimento. As plantas que apresentaram sintomas até os 23 dias após a emergência (DAE) não produziram qualquer tipo de frutos. FC foram colhidos de plantas que apresentaram sintomas a partir dos 42 DAE. Mesmo assim, houve redução de 78,5 % na produção de FC. Plantas que mostraram sintomas por ocasião da última colheita (55 DAE) apresentaram redução na produção de FC de 9,6 %. A infecção com o ZLCV até o início da frutificação inviabiliza a produção de FC de abobrinha de moita 'Caserta'.

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Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus

Scientia Agricola ◽

10.1590/s0103-90162003000400013 ◽

2003 ◽

Vol 60 (4) ◽

pp. 691-698 ◽

Cited By ~ 8

Author(s):

Davi Andrade Pacheco ◽

Jorge Alberto Marques Rezende ◽

Sônia Maria de Stefano Piedade

Keyword(s):

Virus Type ◽

Aerial Part ◽

Mosaic Disease ◽

Ringspot Virus ◽

Virus Concentration ◽

Papaya Ringspot Virus ◽

Severe Strain ◽

Dry Biomass ◽

Marketable Fruit ◽

Cotyledonary Stage

Pre-immunization with mild strains of Papaya ringspot virus - type W (PRWV-W) has allowed the mosaic disease to be controlled in different cucurbit species, with increases in marketable fruit yield. The objective of this study was to compare virus concentration, biomass and symptomatology of 'Caserta' zucchini squash, 'Menina Brasileira' long-neck squash and 'Crimson Sweet' watermelon plants infected by three mild strains and one severe strain of PRSV-W. Plants were inoculated at the cotyledonary stage, under greenhouse conditions, sampled at 7, 14, 21, 28 and 35 days after inoculation (DAI), and analyzed by PTA-ELISA. The severity of the symptoms was scored according to a scale from 1 to 5, and the fresh and dry biomass of the aerial part of the plants were evaluated at 40 DAI. Concentrations of the mild strains, based on absorbance values of the PTA-ELISA, were lower than the concentration of the severe strain for all species. The mild strains did not cause mosaic in infected plants of all species. Plants of zucchini squash and watermelon infected by the severe strain exhibited severe mosaic symptoms, but the same was not noticed for infected long-neck squash plants. Biomass values from zucchini squash and watermelon plants infected by the mild strains were 1.7 % to 12.4 % lower as compared to healthy plants. Biomass values of zucchini squash and watermelon plants infected by the severe strain presented greater reduction, varying from 29 % to 74 %. However, biomass values of long-neck squash plants infected by the mild and severe strains were similar for all treatments.

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NIa-pro of Papaya ringspot virus interacts with papaya methionine sulfoxide reductase B1

Virology ◽

10.1016/j.virol.2012.09.007 ◽

2012 ◽

Vol 434 (1) ◽

pp. 78-87 ◽

Cited By ~ 12

Author(s):

Le Gao ◽

Wentao Shen ◽

Pu Yan ◽

Decai Tuo ◽

Xiaoying Li ◽

...

Keyword(s):

Methionine Sulfoxide ◽

Ringspot Virus ◽

Methionine Sulfoxide Reductase ◽

Papaya Ringspot Virus

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Problemas na premunização de melancia para o controle do mosaico causado pelo Papaya ringspot virus

Fitopatologia Brasileira ◽

10.1590/s0100-41582001000300013 ◽

2001 ◽

Vol 26 (3) ◽

pp. 651-654 ◽

Cited By ~ 4

Author(s):

PAULO R. P. DIAS ◽

JORGE A. M. REZENDE

Keyword(s):

Citrullus Lanatus ◽

Ringspot Virus ◽

Papaya Ringspot Virus

Neste trabalho estudou-se a proteção de melancia (Citrullus lanatus) com estirpes fracas do Papaya ringspot virus, estirpe melancia, em condições de casa de vegetação e em campo. Os resultados mostraram que a proteção foi eficiente em ambos os testes. Nos testes em campo porém, as plantas protegidas da cultivar Crimson Sweet tiveram uma redução no peso médio de frutos por planta de, aproximadamente, 50% e 10,8%, em comparação às plantas não protegidas que estavam sadias, no primeiro e no segundo teste, respectivamente. O mesmo fato ocorreu com a cultivar Crimson Tide, na qual se registrou uma redução de 36,4%, aproximadamente, em um único teste. Além da redução na produção, outro problema encontrado na premunização da melancia foi a necessidade de duas inoculações sucessivas das mudas, em dias consecutivos, para aumentar a freqüência de plantas infetadas.

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