Experimental Cryptorchidism Causes Chronic Inflammation and a Progressive Decline in Sertoli Cell and Leydig Cell Function in the Adult Rat Testis

2021 ◽  
Author(s):  
Rashid A. Aldahhan ◽  
Peter G. Stanton ◽  
Helen Ludlow ◽  
David M. de Kretser ◽  
Mark P. Hedger
Keyword(s):  
Chronic Inflammation ◽  
Sertoli Cell ◽  
Leydig Cell ◽  
Cell Function ◽  
Progressive Decline ◽  
Rat Testis ◽  
Adult Rat ◽  
Leydig Cell Function

FSH regulates cultured Leydig cell function via Sertoli cell proteins: An in vitro study

1985 ◽  
Vol 132 (2) ◽  
pp. 729-734 ◽  
Author(s):  
M. Benahmed ◽  
C. Grenot ◽  
E. Tabone ◽  
P. Sanchez ◽  
A.M. Morera
Keyword(s):  
Sertoli Cell ◽  
Leydig Cell ◽  
Cell Function ◽  
In Vitro Study ◽  
Vitro Study ◽  
Leydig Cell Function

Adult rat Sertoli cells secrete a factor or factors which modulate Leydig cell function

1987 ◽  
Vol 114 (3) ◽  
pp. 459-467 ◽  
Author(s):  
V. Papadopoulos ◽  
P. Kamtchouing ◽  
M. A. Drosdowsky ◽  
M. T. Hochereau de Reviers ◽  
S. Carreau
Keyword(s):  
Cyclic Amp ◽  
Sertoli Cell ◽  
Leydig Cell ◽  
Sertoli Cells ◽  
Leydig Cells ◽  
Cell Function ◽  
Cell Interaction ◽  
Adult Rats ◽  
Adult Rat ◽  
Stimulating Factor

ABSTRACT Production of testosterone and oestradiol-17β by Leydig cells from adult rats was stimulated by LH or dibutyryl cyclic AMP (10 and 2·5-fold respectively). The addition of spent medium from normal, hemicastrated or γ-irradiated rat seminiferous tubule cultures, as well as from Sertoli cell cultures, to purified Leydig cells further enhanced both basal (44 and 53% for testosterone and oestradiol-17β respectively) and LH-stimulated (56 and 18%) steroid output. Simultaneously, a decrease (20–30%) in intracellular cyclic AMP levels was observed. This stimulating factor (or factors) secreted by the Sertoli cells is different from LHRH, is of proteinic nature and has a molecular weight ranging between 10 000 and 50 000; its synthesis is not controlled by FSH nor by testosterone. This factor(s) involved in rat Leydig cell steroidogenesis, at a step beyond the adenylate cyclase, does not require protein synthesis for testosterone formation whereas it does for oestradiol-17β production. It should be noted that a germ cell–Sertoli cell interaction modulates the synthesis of this factor(s). J. Endocr. (1987) 114, 459–467

Leydig Cell Function and Morphology in the Rat Testis after Exposure to Heat

Andrologia ◽  
2009 ◽  
Vol 12 (1) ◽  
pp. 12-19 ◽  
Author(s):  
J.E. DAMBER ◽  
A. BERGH ◽  
P.O. JANSON
Keyword(s):  
Leydig Cell ◽  
Cell Function ◽  
Rat Testis ◽  
Leydig Cell Function

Synergistic Effects of Sertoli Cell and FSH on Leydig Cell Function: In Vitro Study

1984 ◽  
Vol 438 (1 Hormonal Cont) ◽  
pp. 684-687 ◽  
Author(s):  
M. BENAHMED ◽  
J. REVENTOS ◽  
E. TABONE ◽  
J. M. SAEZ
Keyword(s):  
Sertoli Cell ◽  
Leydig Cell ◽  
Cell Function ◽  
Synergistic Effects ◽  
In Vitro Study ◽  
Vitro Study ◽  
Leydig Cell Function

Cultured Sertoli cell-mediated FSH stimulatory effect on Leydig cell steroidogenesis

1985 ◽  
Vol 248 (2) ◽  
pp. E176-E181
Author(s):  
M. Benahmed ◽  
J. Reventos ◽  
E. Tabone ◽  
J. M. Saez
Keyword(s):  
Sertoli Cell ◽  
Leydig Cell ◽  
Sertoli Cells ◽  
Leydig Cells ◽  
Cell Function ◽  
Cell Activity ◽  
Defined Medium ◽  
Cell Number ◽  
Two Parameters ◽  
Leydig Cell Function

To determine the precise role of Sertoli cells in the stimulating effects of follicle stimulating hormone (FSH) on Leydig cell activity, porcine purified Leydig and Sertoli cells were cultured separately or together in a chemically defined medium in the absence or presence of porcine, FSH 50 ng/ml. Leydig cell activity was evaluated using two parameters: human chorionic gonadotropin (hCG) binding sites; and hCG-stimulated cAMP production and testosterone secretion. First, it was found that FSH increases Leydig cell activity in crude Leydig cell preparations (40–60% of Leydig cells), whereas it exerts no effect on purified Leydig cells (greater than 90% of Leydig cells). Second, FSH stimulates the activity of Leydig cells cocultured with Sertoli cells, whereas it remains without effect on purified Leydig cells cultured alone. This stimulating effect of FSH on Leydig cell activity is dependent on the Sertoli cell number in the coculture. These data 1) show that the stimulating effect of FSH on Leydig cell function is mediated by Sertoli cells and 2) support the concept of local control of Leydig cell function originating from Sertoli cells.

Sertoli cell function declines earlier than leydig cell function in aging Japanese men

Maturitas ◽  
1994 ◽  
Vol 18 (2) ◽  
pp. 143-153 ◽  
Author(s):  
Masafumi Haji ◽  
Seiichi Tanaka ◽  
Yoshihiro Nishi ◽  
Toshihiko Yanase ◽  
Ryoichi Takayanagi ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Leydig Cell ◽  
Cell Function ◽  
Japanese Men ◽  
Leydig Cell Function

scholarly journals Noonan syndrome males display Sertoli cell-specific primary testicular insufficiency

2018 ◽  
Vol 179 (6) ◽  
pp. 409-418 ◽  
Author(s):  
Sophie Moniez ◽  
Catherine Pienkowski ◽  
Benoit Lepage ◽  
Safouane Hamdi ◽  
Myriam Daudin ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Leydig Cell ◽  
Noonan Syndrome ◽  
Cell Function ◽  
Retrospective Chart Review ◽  
Inhibin B ◽  
Healthy Children ◽  
Few Data ◽  
Testicular Insufficiency ◽  
Leydig Cell Function

Context Abnormalities in the hypothalamo–pituitary–gonadal axis have long been reported in Noonan syndrome (NS) males with only few data available in prepubertal children. Objective The aim of this study was to describe the gonadal function of NS males from childhood to adulthood. Design It is a retrospective chart review. Patients and methods A total of 37 males with a genetically confirmed diagnosis of NS were included. Clinical and genetic features, as well as serum hormone levels (LH, FSH, testosterone, anti-Müllerian hormone (AMH), and inhibin B) were analysed. Results Of the 37 patients, 16 (43%) children had entered puberty at a median age of 13.5 years (range: 11.4–15.0 years); age at pubertal onset was negatively correlated with BMI SDS (r = −0.541; P = 0.022). In pubertal boys, testosterone levels were normal suggesting a normal Leydig cell function. In contrast, NS patients had significant lower levels of AMH (mean SDS: −0.6 ± 1.1; P = 0.003) and inhibin B (mean SDS: −1.1 ± 1.2; P < 0.001) compared with the general population, suggesting a Sertoli cell dysfunction. Lower AMH and inhibin B levels were found in NS-PTPN11 patients, whereas these markers did not differ from healthy children in SOS1 patients. No difference was found between cryptorchid and non-cryptorchid patients for AMH and inhibin B levels (P = 0.43 and 0.62 respectively). Four NS-PTPN11 patients had a severe primary hypogonadism with azoospermia/cryptozoospermia. Conclusions NS males display Sertoli cell-specific primary testicular insufficiency, whereas Leydig cell function seems to be unaffected.

Sign in / Sign up

Export Citation Format

Share Document