Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine

1980 ◽  
Vol 106 (1) ◽  
pp. 207-212 ◽  
Author(s):  
Owen W. Griffith
Keyword(s):  
Glutathione Reductase ◽  
Glutathione Disulfide

Chip-based SALDI-MS for rapid determination of intracellular ratios of glutathione to glutathione disulfide

2018 ◽  
Vol 62 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Min Li ◽  
Sifeng Mao ◽  
Shiqi Wang ◽  
Hai-Fang Li ◽  
Jin-Ming Lin
Keyword(s):  
Rapid Determination ◽  
Glutathione Disulfide ◽  
Saldi Ms

Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: A comparison with fluorescence detection

2011 ◽  
Vol 76 (4) ◽  
pp. 277-294 ◽  
Author(s):  
Roman Kanďár ◽  
Pavla Žáková ◽  
Miroslava Marková ◽  
Halka Lotková ◽  
Otto Kučera ◽  
...  
Keyword(s):  
Detection Limit ◽  
Whole Blood ◽  
Trichloroacetic Acid ◽  
Signal To Noise Ratio ◽  
Glutathione Disulfide ◽  
Simple Method ◽  
Human Whole Blood ◽  
Coulometric Detection ◽  
Coefficients Of Variation

We describe a relatively simple method for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in human whole blood. We have used an HPLC with coulometric electrochemical detection for the simultaneous measurement of GSH and GSSG. Diluted and filtered trichloroacetic acid extracts were injected directly into the HPLC system and were eluted isocratically on a Polaris 5u C18-A, 250 × 4.6 mm analytical column. Glutathione in samples extracted with trichloroacetic acid and diluted with 1.0 mMhydrochloric acid was stable at 4 °C for at least 8 h. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked whole blood samples were at intervals 91.6–97.6% for GSH and 85.0–104.4% for GSSG. The linear range is 5.0–2000.0 μmol/l, with a detection limit of 2.1 μmol/l (signal-to-noise ratio = 3) for GSH and 2.0–250.0 μmol/l, with a detection limit of 0.9 μmol/l for GSSG.

Determination of glutathione and glutathione disulfide in biological samples: An in-depth review

2009 ◽  
Vol 877 (28) ◽  
pp. 3331-3346 ◽  
Author(s):  
Péter Monostori ◽  
Gyula Wittmann ◽  
Eszter Karg ◽  
Sándor Túri
Keyword(s):  
Biological Samples ◽  
Glutathione Disulfide
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