Quantitation of S-adenosylhomocysteine hydrolase in mouse L929 cells using the inhibitor neplanocin A

1985 ◽  
Vol 149 (1) ◽  
pp. 191-196 ◽  
Author(s):  
Ronnda L. Bartel ◽  
Ronald T. Borchardt
Keyword(s):  
L929 Cells ◽  
Adenosylhomocysteine Hydrolase ◽  
Neplanocin A

Synthesis and Biological Evaluation of Halo-neplanocin A as Novel Mechanism-Based Inhibitors of S-Adenosylhomocysteine Hydrolase

ChemInform ◽  
2004 ◽  
Vol 35 (2) ◽  
Author(s):  
Lak Shin Jeong ◽  
Hyung Ryong Moon ◽  
Jae Gyu Park ◽  
Dae Hong Shin ◽  
Won Jun Choi ◽  
...  
Keyword(s):  
Biological Evaluation ◽  
Adenosylhomocysteine Hydrolase ◽  
Neplanocin A ◽  
Synthesis And Biological Evaluation

Oxidation of neplanocin A to the corresponding 3'-keto derivative by S-adenosylhomocysteine hydrolase

1989 ◽  
Vol 32 (7) ◽  
pp. 1415-1418 ◽  
Author(s):  
Steven D. Paisley ◽  
Michael S. Wolfe ◽  
Ronald T. Borchardt
Keyword(s):  
Adenosylhomocysteine Hydrolase ◽  
Neplanocin A ◽  
Keto Derivative

(1′R, 2′S, 3′R)-9-(2′, 3′-Dihydroxycyclopentan-1′-yl)-Adenine and −3-Deaza-Adenine: Analogues of Aristeromycin Which Exhibit Potent Antiviral Activity with Reduced Cytotoxicity

1993 ◽  
Vol 4 (4) ◽  
pp. 245-248 ◽  
Author(s):  
M. Hasobe ◽  
H. Liang ◽  
D. B. Ault-Riche ◽  
D. R. Borcherding ◽  
M. S. Wolfe ◽  
...  
Keyword(s):  
Cell Growth ◽  
Parent Compound ◽  
Molecular Target ◽  
Adenosine Kinase ◽  
Separate Study ◽  
Synthetic Analogues ◽  
L929 Cells ◽  
Adenosylhomocysteine Hydrolase ◽  
Antiviral Effects ◽  
Adenine Analogues

Two synthetic analogues of aristeromycin, which were shown in a separate study to be inhibitors of S-adenosylhomocysteine hydrolase and devoid of substrate activity with adenosine kinase and adenosine deaminase, were found in this study to inhibit vaccinia virus replication in murine L929 cells and to have reduced cytotoxicity compared with that of the parent compound. Aristeromycin was shown to produce cytocidal effects on murine L929 cells, whereas the synthetic analogues produced cytostatic effects on cell growth. The antiviral effects of these synthetic analogues are correlated with their ability to elevate the intracellular ratio of S-adenosylhomocysteine/ S-adenosylmethionine. These results confirm that S-adenosylhomocysteine hydrolase is the molecular target which mediates the antiviral effects of aristeromycin and that transformation of aristeromycin by cellular adenosine kinase mediates its cytocidal properties.

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