scholarly journals Erratum to “Inhibition by Ca2+ of the hydrolysis and the synthesis of ATP in Ehrlich ascites tumour mitochondria: relation to the Crabtree effect” [Biochim. Biophys. Acta 1228 (1995) 261–266]

1995 ◽  
Vol 1231 (1) ◽  
pp. 121
Author(s):  
K. Bogucka ◽  
V.V. Teplova ◽  
L. Wojtczak ◽  
Yu.V. Evtodienko
1993 ◽  
Vol 40 (4) ◽  
pp. 539-544 ◽  
Author(s):  
A Czyz ◽  
V V Teplova ◽  
P Sabała ◽  
M Czarny ◽  
Y V Evtodienko ◽  
...  

Concentration of free cytoplasmic Ca2+ ([Ca2+]i) in Ehrlich ascites tumour cells loaded with fura-2 was measured in single cells applying a video imaging system. In resting cells [Ca2+]i amounted to 60-340 nM and was increased after addition of 10 mM D-glucose or D-2-deoxyglucose by 80-200 nM. This increase occurred within 30-60 s following addition of the sugars and lasted for several minutes. Pretreatment of the cells with thapsigargin resulted in a much smaller [Ca2+]i increase after addition of glucose or deoxyglucose and, vice versa, thapsigargin added after the sugars mobilized less Ca2+ than when added before. A possible relation of the [Ca2+]i rise evoked by glucose and deoxyglucose to the Crabtree effect is discussed.


2005 ◽  
Vol 5 (4) ◽  
pp. 679-688 ◽  
Author(s):  
Claudia Bincoletto ◽  
Samara Eberlin ◽  
Camila A.V. Figueiredo ◽  
Marcos B. Luengo ◽  
Mary L.S. Queiroz

1976 ◽  
Vol 160 (1) ◽  
pp. 121-123 ◽  
Author(s):  
T L Spencer

The transport and oxidation of succinate by functionally intact Ehrlich ascites-tumour cells was investigated. On the basis of pH dependence and inhibitor sensitivity it was concluded that succinate may be transported across the cell membrane by the organic anion carrier system. Thus the ability of isolated Ehrlich cells to oxidize succinate is real, and is not necessarily a result of damage to cell integrity.


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