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Lab on a Chip ◽  
2022 ◽  
Author(s):  
Andreas Link ◽  
John S McGrath ◽  
Mustafa Zaimagaoglu ◽  
Thomas Franke

We demonstrate the use of an acoustic device to actively encapsulate single red blood cells into individual droplets in a T-junction. We compare the active encapsulation with the passive encapsulation...


2021 ◽  
Author(s):  
Zi yu Gao ◽  
Zhan hao Chang ◽  
Tian Song ◽  
Dong fan Liu ◽  
Xin Li ◽  
...  

Abstract Fibromyalgia (FM) is a confounding factor for diagnosing and assessing rheumatic disease activity. This study sought to assess the extent of this syndrome in rheumatoid arthritis (RA) patients at our rheumatology department. The RA patients were divided into 2 groups (RA with FM and RA without FM) according to the score of the FiRST questionnaire and modified 2016 criteria for FM. We compared the clinical data and disease activities of RA patients with and without FM. As a result, RA patients with FM showed higher levels of CRP, ESR, DAS28-ESR compared with RA patients without FM in both FiRST questionnaires and questionnaires developed to diagnose FM(2016 criteria).Furthermore, RA patients with FM showed higher levels of IgA compared to without FM. For the blood cells count, RA patients with FM showed higher levels of white blood cells, platelets and lower levels of hemoglobin compared with RA patients without FM. Only by FiRST Questionnaires, RA patients with FM showed higher levels of RF compared to without FM. However, all groups showed a similar pattern in anti-CCP and IgG, IgM. RA patients with FM showed lower levels of vitamin D (VD) and higher levels of interleukin (IL)-6 compared with RA patients without FM.In conclusion,FM is a common feature in RA, more associated with high values of disease activity such as ESR, CRP and DAS28-ESR.


2021 ◽  
Vol 2 (2) ◽  
pp. 27
Author(s):  
Catharina Natasa Bella Fortuna ◽  
Franky Chandra Satria Arisgraha, S.T., M.T. ◽  
Puspa Erawati

Based on various epidemiological studies, it is stated that blood lipids are the main risk factor for atherosclerosis that leads to coronary heart disease. In patients with blood lipid disorders, red blood cells undergo deformability so that their shape is flatter than normal red blood cells, which are round. The research entitled Application of Artificial Neural Network Method as Detection of Blood Fat Abnormalities in Image of Complete Blood Examination Results was conducted to help facilitate laboratory examinations. This research hopes that it will provide appropriate early detection to support the expert diagnosis. This research consists of two stages. The first stage is digital image processing to obtain area, perimeter, and eccentricity features. These three features will be used as input to the Backpropagation Neural Network program as the second stage. At this stage, blood lipid abnormalities are detected from features that have been obtained from image processing. The accuracy of detecting blood lipid abnormalities with ANN Backpropagation is 85%.


Agricultura ◽  
2021 ◽  
Vol 18 (1-2) ◽  
pp. 1-8
Author(s):  
Peter-Damian Chukwunomso JIWUBA ◽  
Stanley Uzochukwu ILO ◽  
Wisdom AMADURUONYE ◽  
Nonye Lilian AZODO ◽  
Chinenye UZOMA

The effect of feeding pro-vitamin A cassava peel meal (PCPM) based diets to growing rabbits (n=48) for 61 days on their haematology and serum biochemical parameters was investigated. T1, T2, T3, and T4 diets were formulated with inclusion levels of 0, 15, 30, and 45%, respectively. In a completely randomized design, the rabbits were randomly allocated to four experimental groups of twelve animals each, with four rabbits constituting a replicate. On the last day of the study, blood samples were taken from each animal and analyzed for haematological and serum biochemical indices. The results of the proximate composition of experimental diets revealed that T2, T3, and T4 had high (p<0.05) ash values. T1 diet had higher (p<0.05) metabolizable energy (ME) in comparison with T3, and T4 diets. Red blood cells (RBC) and white blood cells (WBC) were improved (p<0.05) significantly in 15, 30, and 45% PCPM inclusions. T3 and T4 had better (p<0.05) packed cell volume (PCV) values, haemoglobin (Hb), mean cell haemoglobin (MCH), and mean cell haemoglobin concentration (MCHC) than T1 and T2. The treatment groups (T2, T3 and T4) had significantly higher (p<0.05) total protein, globulin, creatinine, total bilirubin, and aspartate amino transferase (AST). Cholesterol and urea levels were lowered significantly (p<0.05) in T3 and T4. All of the blood parameters were within the normal physiological range for clinically healthy rabbits, indicating that PCPM was beneficial to the rabbits' blood formation and health. T4 group had the best results and was recommended for enhanced rabbit production.


2021 ◽  
Vol 9 ◽  
Author(s):  
Viviana Clavería ◽  
Philippe Connes ◽  
Luca Lanotte ◽  
Céline Renoux ◽  
Philippe Joly ◽  
...  

Red blood cells in sickle cell anemia (sRBC) are more heterogeneous in their physical properties than healthy red blood cells, spanning adhesiveness, rigidity, density, size, and shape. sRBC with increased adhesiveness to the vascular wall would trigger vaso-occlusive like complications, a hallmark of sickle cell anemia. We investigated whether segregation occurs among sRBC flowing in micron-sized channels and tested the impact of aggregation on segregation. Two populations of sRBC of different densities were separated, labeled, and mixed again. The mixed suspension was flowed within glass capillary tubes at different pressure-drops, hematocrit, and suspending media that promoted or not cell aggregation. Observations were made at a fixed channel position. The mean flow velocity was obtained by using the cells as tracking particles, and the cell depleted layer (CDL) by measuring the distance from the cell core border to the channel wall. The labeled sRBC were identified by stopping the flow and scanning the cells within the channel section. The tube hematocrit was estimated from the number of fluorescence cells identified in the field of view. In non-aggregating media, our results showed a heterogeneous distribution of sRBC according to their density: low-density sRBC population remained closer to the center of the channel, while the densest cells segregated towards the walls. There was no impact of the mean flow velocity and little impact of hematocrit. This segregation heterogeneity could influence the ability of sRBC to adhere to the vascular wall and slow down blood flow. However, promoting aggregation inhibited segregation while CDL thickness was enhanced by aggregation, highlighting a potential protective role against vaso-occlusion in patients with sickle cell anemia.


2021 ◽  
Author(s):  
Choukri Mamoun ◽  
Anasuya C. Pal ◽  
Isaline Renard ◽  
Pallavi Singh ◽  
Pratap Vydyam ◽  
...  

Hematozoa are a subclass of protozoan parasites that invade and develop within vertebrate red blood cells to cause the pathological symptoms associated with diseases of both medical and veterinary importance such as malaria and babesiosis. A major limitation in the study of the most prominent hematozoa, Plasmodium spp, the causative agents of malaria, is the lack of a broadly accessible mouse model to evaluate parasite infection in vivo as is the case for P. falciparum or altogether the lack of an in vitro culture and mouse models as is the case for P. vivax, P. malariae and P. ovale. Similarly, no in vitro culture system exists for Babesia microti, the predominant agent of human babesiosis. In this study, we show that human red blood cells infected with the human pathogen Babesia duncani continuously propagated in culture, as well as merozoites purified from parasite cultures, can cause lethal infection in immunocompetent C3H/HeJ mice. Furthermore, highly reproducible parasitemia and survival outcomes were established using specific parasite loads and different mouse genetic backgrounds. Using the combined in culturein mouse (ICIM) model of B. duncani infection, we demonstrate that current recommended combination therapies for the treatment of human babesiosis, while synergistic in cell culture, have weak potency in vitro and failed to clear infection or prevent death in mice. Interestingly, using the ICIM model, we identified two new endochin-like quinolone prodrugs, ELQ-331 and ELQ468, that alone or in combination with atovaquone are highly efficacious against B. duncani and B. microti. The novelty, ease of use and scalability of the B. duncani ICIM dual model make it an ideal system to study intraerythrocytic parasitism by protozoa, unravel the molecular mechanisms underlying parasite virulence and pathogenesis, and accelerate the development of innovative therapeutic strategies that could be translated to unculturable parasites and important pathogens for which an animal model is lacking.


Life ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1331
Author(s):  
Ana Bura ◽  
Antonija Jurak Begonja

Phosphoinositides (PIs) are phosphorylated membrane lipids that have a plethora of roles in the cell, including vesicle trafficking, signaling, and actin reorganization. The most abundant PIs in the cell are phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] and phosphatidylinositol-4-monophosphate (PI4P). The localization and roles of both PI(4,5)P2 and PI4P are well established, is the broadly accepted methodological approach for their immunocytochemical visualization in different cell compartments in several cell lines. However, not much is known about these PIs in platelets (PLTs), the smallest blood cells that detect vessel wall injury, activate, and stop the bleeding. Therefore, we sought to investigate the localization of PI(4,5)P2 and PI4P in resting and activated PLTs by antibody staining. Here, we show that the intracellular pools of PI(4,5)P2 and PI4P can be detected by the established staining protocol, and these pools can be modulated by inhibitors of OCRL phosphatase and PI4KIIIα kinase. However, although resting PLTs readily stain for the plasma membrane (PM) pools of PI(4,5)P2 and PI4P, just a few activated cells were stained with the established protocol. We show that optimized protocol allows for the visualization of PI(4,5)P2 and PI4P at PM in activated PLTs, which could also be modulated by OCRL and PI4KIIIα inhibitors. We conclude that PI(4,5)P2 and PI4P are more sensitive to lipid extraction by permeabilizing agents in activated than in resting human PLTs, which suggests their different roles during PLT activation.


Sensors ◽  
2021 ◽  
Vol 21 (23) ◽  
pp. 8043
Author(s):  
Hyuntae Cho ◽  
Seung-Ro Lee ◽  
Yunju Baek

Anemia is a condition in which red blood cells or the hemoglobin in the blood is lower than in healthy people. Red blood cells transport and supply oxygen needed to various organs in the human body. Anemia is caused by hypoxemia due to the lack of red blood cells and causes other serious health problems, such as heart problems, pregnancy complications, severe fatigue, or death. There are many causes of anemia, and it can be diagnosed by measuring hematocrit or hemoglobin levels in the blood. Even though there are various diagnostic devices on the market, these devices are inconvenient because their systems are bulky, heavy, expensive, or inaccurate. This study proposed a new anemia diagnostic system based on the impedance measurement of red blood cells. The proposed system consists of a test strip that collects a blood sample from the finger and a hemoglobin meter that measures the impedance of the blood and converts it into the concentration of hemoglobin. The proposed test strip that does not contain enzymes or reagents was designed in accordance with class 1 approval by the Food and Drug Administration (FDA). The hemoglobin meter was designed to include a hardware block, an algorithm block and a calibration block through empirical work. We also compared it to reference impedance to prove the accuracy of the hemoglobin meter. The experimental results with human blood indicated the superiority of the anemia diagnostic system. As a result, the overall standard deviation of impedance measurements was less than 1%, and the coefficient of variance of the proposed system was 1.7%, which was better than that of other commercial systems.


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