The isolation and characterization of plasma membrane from cultured cells V. The chemical composition of plasma membranes isolated from chicken tumors initiated with virus-transformed cells

1973 ◽  
Vol 298 (4) ◽  
pp. 817-826 ◽  
Author(s):  
James F. Perdue ◽  
David Warner ◽  
Katherine Miller
Keyword(s):  
Plasma Membrane ◽  
Chemical Composition ◽  
Plasma Membranes ◽  
Cultured Cells ◽  
Transformed Cells ◽  
Isolation And Characterization

scholarly journals The Isolation and Characterization of Plasma Membranes from Cultured Cells

1971 ◽  
Vol 246 (22) ◽  
pp. 6750-6759
Author(s):  
James F. Perdue ◽  
With the technical assistance of Cynthia Coda
Keyword(s):  
Plasma Membranes ◽  
Cultured Cells ◽  
Isolation And Characterization

The isolation and characterization of plasma membrane from cultured cells

1972 ◽  
Vol 266 (2) ◽  
pp. 505-510 ◽  
Author(s):  
James F. Perdue ◽  
Rolf Kletzien ◽  
Virginia Lee Wray
Keyword(s):  
Plasma Membrane ◽  
Cultured Cells ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of plasma membranes from wild type Neurospora crassa.

1981 ◽  
Vol 256 (23) ◽  
pp. 12336-12342 ◽  
Author(s):  
E.J. Bowman ◽  
B.J. Bowman ◽  
C.W. Slayman
Keyword(s):  
Neurospora Crassa ◽  
Plasma Membranes ◽  
Wild Type ◽  
Isolation And Characterization

Isolation and characterization of plasma membrane Na+/H+ antiporter genes from salt-sensitive and salt-tolerant reed plants

2009 ◽  
Vol 166 (3) ◽  
pp. 301-309 ◽  
Author(s):  
Ryuichi Takahashi ◽  
Shenkui Liu ◽  
Tetsuo Takano
Keyword(s):  
Plasma Membrane ◽  
Salt Tolerant ◽  
Isolation And Characterization ◽  
Reed Plants

scholarly journals Resolution of diaminobenzidine for the detection of horseradish peroxidase on surfaces of cultured cells.

1985 ◽  
Vol 33 (8) ◽  
pp. 837-839 ◽  
Author(s):  
A Messing ◽  
A Stieber ◽  
N K Gonatas
Keyword(s):  
Plasma Membrane ◽  
Horseradish Peroxidase ◽  
Plasma Membranes ◽  
Cultured Cells ◽  
Lateral Spread ◽  
Ciliary Ganglion ◽  
Monolayer Cultures ◽  
Indirect Immunoperoxidase ◽  
Ciliary Ganglion Neurons ◽  
Ganglion Neurons

The resolution of indirect immunoperoxidase methods for localizing antigens on the surface of plasma membranes of cultured cells was tested using dissociated monolayer cultures of ciliary ganglion neurons prelabeled with cationic ferritin. Clusters of ferritin were produced on the cell surface by warming the cells to 37 degrees C after the ferritin, rabbit anti-ferritin, and goat anti-rabbit immunoglobulin coupled to horseradish peroxidase had all been applied. Intense 3,3'-diaminobenzidine tetrahydrochloride (DAB) staining was limited to the regions immediately surrounding the ferritin clusters. The lateral spread of the DAB reaction product beyond the outer ferritin particles in each cluster averaged 54-81 nm in four experiments. A second type of increased density, coinciding with the thickness of the plasma membrane, was also seen. These stained plasma membranes extended 161-339 nm from the ferritin clusters.

Isolation and characterization of plasma membrane from lactating bovine mammary gland

1982 ◽  
Vol 689 (1) ◽  
pp. 121-134 ◽  
Author(s):  
Choemon Kanno ◽  
Hiroyuki Hattori ◽  
Kunio Yamauchi
Keyword(s):  
Plasma Membrane ◽  
Mammary Gland ◽  
Bovine Mammary Gland ◽  
Isolation And Characterization

scholarly journals Paracrystalline arrays of membrane-to-membrane cross bridges associated with the inner surface of plasma membrane

1978 ◽  
Vol 77 (2) ◽  
pp. 323-328 ◽  
Author(s):  
WW Franke ◽  
C Grund ◽  
E Schmid ◽  
E Mandelkow
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Cultured Cells ◽  
Membrane Surface ◽  
Hexagonal Lattice ◽  
Interparticle Distance ◽  
Macromolecular Complexes ◽  
Inner Surface ◽  
Cross Bridges ◽  
Plasma Membrane Surface

In cultured cells of the rat kangaroo PtK2 line, veils of the cell surface were observed which consisted of only plasma membrane and paracrystalline arrays of membrane-associated particles sandwiched in between. These membrane-to-membrane cross-bridging 9-to 11-nm wide particles were somewhat coumellar-shaped and were arranged on a hexagonal lattice with an interparticle distance of 16nm. At higher magnification, they revealed an unstained core, thus suggesting a ringlike substructure. Similar arrays of paracrystal-containing veils, which were rather variable in size and frequency, were also observed in other cultured cells. It is hypothesized that these paracrystals represent protein macromolecular complexes associated with the inner plasma membrane surface which crystallize when plasma membranes come into close intracellular contact and other components of the subsurface network are removed.

Isolation and characterization of plasma membrane fromAcanthamoeba culbertsoni

1985 ◽  
Vol 7 (3-4) ◽  
pp. 365-373 ◽  
Author(s):  
Sanjay Kumar Mishra ◽  
N. K. Garg ◽  
A. M. Kidwai
Keyword(s):  
Plasma Membrane ◽  
Isolation And Characterization
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