Isolation of a helical, lipid-binding fragment from the human plasma high density lipoprotein, apolp-GLN-I

1972 ◽  
Vol 49 (6) ◽  
pp. 1444-1451 ◽  
Author(s):  
R.L. Jackson ◽  
H.N. Baker ◽  
J.S.K. David ◽  
A.M. Gotto
Keyword(s):  
High Density Lipoprotein ◽  
Human Plasma ◽  
Density Lipoprotein ◽  
Lipid Binding ◽  
High Density ◽  
Plasma High Density Lipoprotein ◽  
Plasma High Density

A new method for the fractionation of human plasma high density lipoprotein

1977 ◽  
Vol 55 (7) ◽  
pp. 766-768 ◽  
Author(s):  
Bernard Rubenstein ◽  
Susan Evans ◽  
George Steiner
Keyword(s):  
High Density Lipoprotein ◽  
Human Plasma ◽  
Density Lipoprotein ◽  
High Density ◽  
New Method ◽  
Continuous Gradient ◽  
Elution Pattern ◽  
Plasma High Density Lipoprotein ◽  
Plasma High Density ◽  
Peptide Composition

We have devised a new method for the fractionation of human plasma high density lipoprotein (HDL). The HDL was chromatographed on DEAE-agarose columns using a continuous gradient of 0.06–0.15 M NaCl. The elution pattern obtained showed three phases, each with differing peptide composition. Examination of the three subfractions showed that each contained both apo A-I and apo A-II, but indifferent proportions. Subfraction 1 contained noapoC-II orC-III-1 and only a trace of apo C-III-2, subfraction 2 contained apo C-II and C-III-1 but no C-III-2, while subfraction 3 contained considerable apo C-III-2 with only traces of apo C-II or C-III-1.

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