A new group of glucocerebrosidase isozymes found in human white blood cells

1980 ◽  
Vol 97 (3) ◽  
pp. 1103-1107 ◽  
Author(s):  
Edward I. Ginns ◽  
Roscoe O. Brady ◽  
Daniel W. Stowens ◽  
F.Scott Furbish ◽  
John A. Barranger
1990 ◽  
Vol 269 (3) ◽  
pp. 723-728 ◽  
Author(s):  
M Wolf ◽  
M Baggiolini

Cytosol and membrane fractions from human neutrophils, monocytes, lymphocytes and platelets were separated by SDS/PAGE, blotted on to nitrocellulose and assayed for selective binding of phosphatidylserine (PS). Two PS-binding proteins with apparent molecular masses of 115 kDa and 100 kDa were identified in the cytosol of neutrophils, monocytes and lymphocytes. Corresponding bands along with other PS-binding proteins were detected in platelets in both cytosol and membrane fractions. These proteins were also found to bind protein kinase C (PKC) provided that PS was present. The 115 kDa and 100 kDa proteins (PS-p115/110) were partially purified from neutrophils and were used for the study of PS and PKC binding. The binding of PS did not require Ca2+ or Mg2+ and was inhibited by phosphatidic acid, by 1-alkyl-2-acetylphosphocholine and, to a lesser extent, by other lipids. The binding of PKC, however, was strictly PS- and Ca2(+)-dependent and seems to occur secondarily to PS binding.


1996 ◽  
Vol 288 (10) ◽  
pp. 570-574 ◽  
Author(s):  
Jarmo K. Laihia ◽  
Jaakko Uksila ◽  
Marko Luhtala ◽  
Christer T. Jansén

2008 ◽  
Vol 42 (3) ◽  
pp. 253-259 ◽  
Author(s):  
Paschalis-Thomas Doulias ◽  
Chara Vlachou ◽  
Christina Boudouri ◽  
Panagiotis Kanavaros ◽  
Kostas C. Siamopoulos ◽  
...  

Author(s):  
R.C. Wilkins ◽  
D. Wilkinson ◽  
H.P. Maharaj ◽  
P.V. Bellier ◽  
M.B. Cybulski ◽  
...  

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