Structural differences between [2Fe-2S] clusters in spinach ferredoxin and in the “Red paramagnetic protein” from Clostridium pasteurianum. A resonance Raman study

1984 ◽  
Vol 119 (3) ◽  
pp. 828-835 ◽  
Author(s):  
Jacques Meyer ◽  
Jean-Marc Moulis ◽  
Marc Lutz

1984 ◽  
Vol 219 (3) ◽  
pp. 829-832 ◽  
Author(s):  
J M Moulis ◽  
J Meyer ◽  
M Lutz

Resonance Raman spectra of native 2[4Fe-4S] ferredoxin from Clostridium pasteurianum and of its selenium-substituted analogue 2[4Fe-4Se] are compared. The experimental conditions used in this study included low temperature (approx. 25K) and the absence of a glass sample cell, thus ensuring high signal-to-noise ratios. The spectra of the 2[4Fe-4S] and 2[4Fe-4Se] ferredoxins display similar numbers of bands, but the resonance Raman patterns differ largely, except for two bands observed at 353 cm-1 and 365 cm-1 in spectra of the native ferredoxin, which are only moderately shifted upon S----Se substitution and are attributed to Fe-S(cysteine) stretching modes. The activities of the latter modes, enhanced by 457.9 nm excitation, are nearly equal in both ferredoxins. These data were used to demonstrate the presence of hybrid clusters [4Fe-(4-n)S-nSe] (n = 1, 2, 3) in a ferredoxin the active sites of which had been reconstituted in the presence of both S and Se.



1997 ◽  
Vol 67 (1-4) ◽  
pp. 49
Author(s):  
G. Fraczkiewicz ◽  
A.R. Nersissian ◽  
J.P. Hart ◽  
J.S. Valentine ◽  
R.S. Czernuszewicz


1987 ◽  
Vol 135 (4-5) ◽  
pp. 463-467 ◽  
Author(s):  
S.D. Conradson ◽  
R.F. Dallinger ◽  
B.I. Swanson ◽  
R.J.H. Clark ◽  
V.B. Croud


Biochemistry ◽  
1989 ◽  
Vol 28 (20) ◽  
pp. 8011-8022 ◽  
Author(s):  
Alain Desbois ◽  
Mariella Tegoni ◽  
Michel Gervais ◽  
Marc Lutz


2004 ◽  
Vol 69 (20) ◽  
pp. 6641-6657 ◽  
Author(s):  
Chensheng Ma ◽  
Peng Zuo ◽  
Wai Ming Kwok ◽  
Wing Sum Chan ◽  
Jovi Tze Wai Kan ◽  
...  




2013 ◽  
Vol 419 ◽  
pp. 178-183 ◽  
Author(s):  
Sachiko Yanagisawa ◽  
Masayuki Hara ◽  
Hiroshi Sugimoto ◽  
Yoshitsugu Shiro ◽  
Takashi Ogura


2010 ◽  
Vol 405 (21) ◽  
pp. 4570-4573 ◽  
Author(s):  
Jamal Talla ◽  
Donghui Zhang ◽  
Madhuvanthi Kandadai ◽  
Aditya Avadhanula ◽  
Seamus Curran


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