Comparison of inhibitory activity of various organophosphorus compounds against acetylcholinesterase and neurotoxic esterase of hens with respect to delayed neurotoxicity

1980 ◽  
Vol 29 (20) ◽  
pp. 2721-2727 ◽  
Author(s):  
Hideo Ohkawa ◽  
Hiroko Oshita ◽  
Junshi Miyamoto
Keyword(s):  
Inhibitory Activity ◽  
Organophosphorus Compounds ◽  
Delayed Neurotoxicity ◽  
Neurotoxic Esterase

scholarly journals Characterization of [3H]di-isopropyl phosphorofluoridate-binding proteins in hen brain. Rates of phosphorylation and sensitivity to neurotoxic and non-neurotoxic organophosphorus compounds

1985 ◽  
Vol 228 (3) ◽  
pp. 537-544 ◽  
Author(s):  
C D Carrington ◽  
M B Abou-Donia
Keyword(s):  
Organophosphorus Compound ◽  
Binding Proteins ◽  
Organophosphorus Compounds ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Reaction Rates ◽  
Binding Activity ◽  
Binding Characteristics ◽  
Delayed Neurotoxicity ◽  
Neurotoxic Esterase

The experiments described in this paper were designed to isolate [3H]di-isopropyl phosphorofluoridate-binding proteins by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis for the purpose of characterizing and identifying potential initiation sites for organophosphorus-compound-induced delayed neurotoxicity. The major Paraoxon-insensitive Mipafox-sensitive binding protein (Mr 160 000) was found to be identical with one previously identified as neurotoxic esterase, an enzyme that has been proposed to be the target site for organophosphorus-compound-induced delayed neurotoxicity. However, two other binding proteins with suitable binding characteristics were also found in smaller amounts, one of which has not been detected previously. Di-isopropyl phosphorofluoridate was found to phosphorylate all three of these proteins at rates similar to the rate at which neurotoxic esterase is inhibited by di-isopropyl phosphorofluoridate. Varying the concentration of di-isopropyl phosphorofluoridate or the time of incubation produced similar increases in binding to each of the labelled proteins. This suggests that the reaction rates of di-isopropyl phosphorofluoridate with proteins may be described by first-order kinetics, and the concentration of the Michael is complex formed during binding is minimal for all the phosphorylated proteins. The recovery of the binding activity in the 160 000-Mr band was found to be similar to the recovery of neurotoxic esterase activity, lending further support to the contention that this band is identical with neurotoxic esterase.

scholarly journals Studies on the delayed neurotoxicity of organophosphorus compounds. III.

1985 ◽  
Vol 10 (2) ◽  
pp. 67-82 ◽  
Author(s):  
Hirotoshi ITOH ◽  
Hidemi KISHIDA ◽  
Eiko TAKEUCHI ◽  
Mamoru TADOKORO ◽  
Toshiyuki UCHIKOSHI ◽  
...  
Keyword(s):  
Organophosphorus Compounds ◽  
Delayed Neurotoxicity

Bivalent Organophosphorus Compounds—Synthesis and Acetylcholinesterase Inhibitory Activity

2013 ◽  
Vol 188 (8) ◽  
pp. 1095-1103 ◽  
Author(s):  
Ruliang Xie ◽  
Ting Zhang ◽  
Qianfei Zhao ◽  
Tao Zhang ◽  
Xiangdong Mei ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Organophosphorus Compounds ◽  
Acetylcholinesterase Inhibitory Activity

scholarly journals Studies on the delayed neurotoxicity of organophosphorus compounds- (I)

1981 ◽  
Vol 6 (4) ◽  
pp. 287-300 ◽  
Author(s):  
Hirotoshi ITOH ◽  
Mamoru TADOKORO ◽  
Kiyoshi OIKAWA
Keyword(s):  
Organophosphorus Compounds ◽  
Delayed Neurotoxicity

Test systems for in vivo and in vitro estimation of the risk and development of delayed neurotoxicity caused by organophosphorus compounds

2007 ◽  
Vol 41 (10) ◽  
pp. 516-518
Author(s):  
O. A. Khodakovskaya ◽  
N. A. Vodolazskaya ◽  
L. D. Glukhova ◽  
S. I. Timofeeva ◽  
S. I. Dvoretskaya ◽  
...  
Keyword(s):  
Organophosphorus Compounds ◽  
Delayed Neurotoxicity ◽  
Test Systems
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