Sex difference in responsiveness to aztreonam of monooxygenase system in liver microsomes from rats

1987 ◽  
Vol 36 (7) ◽  
pp. 1053-1057 ◽  
Author(s):  
Tohru Horie ◽  
Mitsukazu Kitada ◽  
Yoshio Tanabe ◽  
Yoshio Kanakubo
Keyword(s):  
Sex Difference ◽  
Liver Microsomes ◽  
Monooxygenase System

Sex difference in the O-dealkylation activity of 7-hydroxycoumarin O-alkyl derivatives in liver microsomes of rats

1980 ◽  
Vol 29 (7) ◽  
pp. 1015-1022 ◽  
Author(s):  
Tetsuya Kamataki ◽  
Mebae Ando ◽  
Yasushi Yamazoe ◽  
Kenji Ishii ◽  
Ryuichi Kato
Keyword(s):  
Sex Difference ◽  
Liver Microsomes ◽  
Alkyl Derivatives

scholarly journals STUDIES ON MECHANISM OF SEX DIFFERENCE IN DRUG-OXIDIZING ACTIVITY OF LIVER MICROSOMES

1968 ◽  
Vol 18 (4) ◽  
pp. 482-489 ◽  
Author(s):  
RYUICHI KATO ◽  
AKIRA TAKANAKA ◽  
MIGHIKO TAKAYANAGI
Keyword(s):  
Sex Difference ◽  
Liver Microsomes

Monooxygenase System and NO Metabolism in Liver Microsomes of Rats with Toxic Hepatitis and the Effect of Sesquiterpene Lactones

2021 ◽  
Vol 172 (2) ◽  
pp. 133-136
Author(s):  
N. V. Tursunova ◽  
V. N. Syrov ◽  
Z. A. Khushbaktova ◽  
Yu. V. Tornuev ◽  
M. G. Klinnikova
Keyword(s):  
Toxic Hepatitis ◽  
Sesquiterpene Lactones ◽  
Liver Microsomes ◽  
Monooxygenase System

A Direct, Highly Sensitive Fluorometric Assay for a Microsomal Cytochrome P450-Mediated O-Demethylation Using a Novel Coumarin Analog as Substrate

2000 ◽  
Vol 55 (11-12) ◽  
pp. 915-922 ◽  
Author(s):  
René Roser ◽  
Helmut Thomas
Keyword(s):  
Rat Liver ◽  
Liver Microsomes ◽  
Fluorometric Assay ◽  
Excitation Wavelength ◽  
Monooxygenase System ◽  
Rat Liver Microsomes ◽  
Monooxygenase Activity ◽  
Continuous Increase ◽  
Highly Sensitive

A highly sensitive fluorometric assay for the determination of monooxygenase activity in liver microsomes is described. The assay is based on the use of 3-chloro-7-methoxy-4-methylcoumarin which is demethylated to 3-chloro-7-hydroxy-4-methylcoumarin. The rate of formation of 3-chloro-7-hydroxy-4-methylcoumarin was recorded as an increase of fluorescence (λA = 380 nm, λF = 480 nm ) with time. When 3-chloro-7-methoxy-4-methylcoumarin was incubated in the presence of MgCl2and NADPH with rat liver microsomes, a continuous increase of the fluorescence could be measured. The reaction proceeded linearly for about 10 min and at least up to a concentration of 0.1 mg/ml of microsomal protein. Besides 3-chloro-7-hydroxy-4-methylcoumarin a hydroxylated derivative of the substrate was formed as a second metabolite during the incubation. Using an excitation wavelength of 480 nm and a fluorescence/em ission w avelength of 480 nm, the fluorescence of this substance (λA = 338 nm, λF = 422 nm ) amounted only to about 1% of the fluorescence of the main product. The use of 3-chloro-7-methoxy-4-methylcoumarin as substrate enables the fluorometric determination of the O-dealkylation activity of a cytochrom e P450-dependent monooxygenase system in rat liver which is inducible by phenobarbital but not by 3-methylcholanthrene.

Position selective sex difference in imipramine metabolism in rat liver microsomes

1985 ◽  
Vol 34 (6) ◽  
pp. 898-900 ◽  
Author(s):  
Masato Chiba ◽  
Eri Nishihara ◽  
Shoichi Fujita ◽  
Tokuji Suzuki
Keyword(s):  
Sex Difference ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Imipramine Metabolism
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