Partial purification and characterization of a rat liver polychlorinated biphenyl (PCB) binding protein

1993 ◽  
Vol 45 (4) ◽  
pp. 885-891 ◽  
Author(s):  
Angelika Bründl ◽  
Klaus Buff
Keyword(s):  
Rat Liver ◽  
Polychlorinated Biphenyl ◽  
Binding Protein ◽  
Partial Purification ◽  
Purification And Characterization

Partial purification and characterization of a membrane-associated steroid-binding protein from Pseudomonas testosteroni

1982 ◽  
Vol 60 (8) ◽  
pp. 798-803 ◽  
Author(s):  
Mike Francis ◽  
Mamoru Watanabe
Keyword(s):  
Binding Protein ◽  
Membrane Vesicles ◽  
Activity Analysis ◽  
Binding Activity ◽  
Regulatory Function ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Steroid Binding ◽  
Steroid Binding Protein

A steroid-binding protein obtained from the supernatant of the final wash from the preparation of membrane vesicles was purified severalfold to near homogeneity. The protein binds C18 and C19 steroids but has the highest affinity for androstenedione (Kd = 1.6 × 10−10 M). The molecular weight is 51 000 – 58 000. Binding activity is slightly inhibited by Cu2+, Ca2+, and Mg2+ and completely inhibited by Zn2+. The protein has no detectable steroid degradative activity. Analysis of androstenedione binding revealed negative cooperativity of binding for this ligand and may indicate a regulatory function for this protein. It is postulated that this protein binds the steroid after testosterone is converted to androstenedione.

Purification and characterization of a novel GTP-binding protein with a Mr value of 24,000 from rat liver

1990 ◽  
Vol 169 (2) ◽  
pp. 816-823 ◽  
Author(s):  
Toshihiko Ohmori ◽  
Yoshifumi Takeyama ◽  
Takashi Ueda ◽  
Motoki Hiroyoshi ◽  
Hiroyuki Nakanishi ◽  
...  
Keyword(s):  
Rat Liver ◽  
Binding Protein ◽  
Purification And Characterization ◽  
Gtp Binding Protein ◽  
Gtp Binding

scholarly journals An Improved Method for the Purification and Characterization of a 54 kDa Protein in Rat Liver Which Has Recently Been Identified as a Selenium-Binding Protein

1999 ◽  
Vol 45 (4) ◽  
pp. 203-208 ◽  
Author(s):  
Takumi Ishida ◽  
Ayako Fukuda ◽  
Yuko Yoshioka ◽  
Daisuke Maji ◽  
Yuji Ishii ◽  
...  
Keyword(s):  
Rat Liver ◽  
Binding Protein ◽  
Improved Method ◽  
Purification And Characterization
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