Partial Purification and Characterization of Copper-binding Protein from Roots of Avena sativa Grown on Excess Copper

1985 ◽  
Vol 120 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Anna Tukendorf ◽  
Tadeusz Baszyński
Keyword(s):  
Avena Sativa ◽  
Binding Protein ◽  
Partial Purification ◽  
Copper Binding ◽  
Purification And Characterization ◽  
Excess Copper ◽  
Copper Binding Protein

Partial purification and characterization of a membrane-associated steroid-binding protein from Pseudomonas testosteroni

1982 ◽  
Vol 60 (8) ◽  
pp. 798-803 ◽  
Author(s):  
Mike Francis ◽  
Mamoru Watanabe
Keyword(s):  
Binding Protein ◽  
Membrane Vesicles ◽  
Activity Analysis ◽  
Binding Activity ◽  
Regulatory Function ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Steroid Binding ◽  
Steroid Binding Protein

A steroid-binding protein obtained from the supernatant of the final wash from the preparation of membrane vesicles was purified severalfold to near homogeneity. The protein binds C18 and C19 steroids but has the highest affinity for androstenedione (Kd = 1.6 × 10−10 M). The molecular weight is 51 000 – 58 000. Binding activity is slightly inhibited by Cu2+, Ca2+, and Mg2+ and completely inhibited by Zn2+. The protein has no detectable steroid degradative activity. Analysis of androstenedione binding revealed negative cooperativity of binding for this ligand and may indicate a regulatory function for this protein. It is postulated that this protein binds the steroid after testosterone is converted to androstenedione.

Partial Purification and Characterization of a Gibberellin-Binding Protein from Seedlings ofAzukia angularis

1997 ◽  
Vol 241 (3) ◽  
pp. 782-786 ◽  
Author(s):  
Masatoshi Nakajima ◽  
Keiji Takita ◽  
Hideki Wada ◽  
Kenji Mihara ◽  
Morifumi Hasegawa ◽  
...  
Keyword(s):  
Binding Protein ◽  
Partial Purification ◽  
Purification And Characterization
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