Cholera toxin and wheat germ agglutinin conjugates as neuroanatomical probes: Their uptake and clearance, transganglionic and retrograde transport and sensitivity

1982 ◽  
Vol 243 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Xuan-Cai S. Wan ◽  
John Q. Trojanowski ◽  
Jacqueline O. Gonatas
Keyword(s):  
Cholera Toxin ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Retrograde Transport

scholarly journals Superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport.

1979 ◽  
Vol 27 (3) ◽  
pp. 728-734 ◽  
Author(s):  
N K Gonatas ◽  
C Harper ◽  
T Mizutani ◽  
J O Gonatas
Keyword(s):  
Horseradish Peroxidase ◽  
Axonal Transport ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Brain Research ◽  
Retrograde Transport ◽  
Retrograde Axonal Transport ◽  
Hypoglossal Nucleus ◽  
Highly Sensitive ◽  
Cervical Ganglion

We have compared the retrograde axonal transport of horseradish peroxidase (HRP), to the retrograde transport of HRP conjugated with wheat germ agglutinin (WGA). Morphometric studies have shown that WGA-HRP conjugates were 40 times more sensitive than free HRP, in the tracing of retrograde connections from the rat submandibular gland to the superior cervical ganglion. Also, WGA-HRP was more sensitive than free HRP in the tracing of retrograde connections from the rat tongue to the hypoglossal nucleus. Our findings with WGA-HRP are consistent with the observations by Schwab et al. who reported (-125I) WGA is a highly sensitive retrograde tracer (Brain Research 152:145, 1978 (22)).

Sign in / Sign up

Export Citation Format

Share Document