An improved method for the determination of acetylcholinesterase activity in rectal biopsy tissue from patients with hirschsprung's disease

1977 ◽  
Vol 77 (3) ◽  
pp. 407-413 ◽  
Author(s):  
G. Dale ◽  
J.R. Bonham ◽  
Katherine W.A. Riley ◽  
J. Wagget
Keyword(s):  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Rectal Biopsy ◽  
Acetylcholinesterase Activity ◽  
Improved Method ◽  
Biopsy Tissue

scholarly journals Experience of acetylcholinesterase histochemistry application in the diagnosis of chronic constipation in children

Medicina ◽  
2006 ◽  
Vol 43 (5) ◽  
pp. 376 ◽  
Author(s):  
Robertas Bagdzevičius ◽  
Vytautas Vaičekauskas ◽  
Sigita Bagdzevičiūtė
Keyword(s):  
False Positive ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Rectal Biopsy ◽  
Acetylcholinesterase Activity ◽  
Intestinal Neuronal Dysplasia ◽  
Biopsy Specimens ◽  
Acetylcholinesterase Histochemistry ◽  
Group 2 ◽  
Neuronal Dysplasia

The aim of this study was to review our experience in applying acetylcholinesterase histochemistry for diagnosing colonic dysganglionoses in children. Patients and methods. We analyzed acetylcholinesterase histochemistry results of rectal biopsy specimens obtained from 85 children. The indications for biopsy were suspicion of Hirschsprung’s disease in neonates and infants (Group 1; n=21) and older children (Group 2; n=17); megarectum (Group 3; n=44); and colostomy (Group 4; n=3). Specimens were taken at 5 and 10 cm using endoscopic forceps or excised with scissors at 2.5 cm above the dentate line. Acetylcholinesterase activity was evaluated using Karnovsky-Roots method. Results. The diagnosis of Hirschsprung’s disease was confirmed in 17 children of the first group and in 3 of the second group. In the third group, 2 children were diagnosed with ultrashort-segment Hirschsprung’s disease and 3 children with intestinal neuronal dysplasia. In one case, acetylcholinesterase reaction was false positive. Hirschsprung’s disease was diagnosed in 2 children with colostomies; in one case acetylcholinesterase activity caused false-positive results. Colonic dysganglionoses were diagnosed in 78% of infants and in 14% of children over 1 year of age. The diagnostic specificity of acetylcholinesterase in Hirschsprung’s disease was 92%. Conclusions. 1) The analysis of acetylcholinesterase activity in children’s rectal biopsy specimens is a reliable method for diagnosing Hirschsprung’s disease, especially in infants; 2) This method of examination is irreplaceable in diagnosing ultrashort-segment Hirschsprung’s disease and remains the only method to confirm the diagnosis of this disease; 3) Acetylcholinesterase histochemistry is not sufficiently informative in diagnosing intestinal neuronal dysplasia type B, because authors applying other neurohistochemical investigation methods have reported higher incidence of this disease.

Acetylcholinesterase activity in suction biopsies of the rectum in the diagnosis of Hirschsprung's disease

1972 ◽  
Vol 7 (1) ◽  
pp. 11-17 ◽  
Author(s):  
W. Meier-Ruge ◽  
P.M. Lutterbeck ◽  
B. Herzog ◽  
R. Morger ◽  
R. Moser ◽  
...  
Keyword(s):  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Acetylcholinesterase Activity

Rectal Biopsy for Hirschsprung's Disease – Are We Performing too Many?

2010 ◽  
Vol 20 (02) ◽  
pp. 95-97 ◽  
Author(s):  
N. Rahman ◽  
J. Chouhan ◽  
S. Gould ◽  
Vt. Joseph ◽  
H. Grant ◽  
...  
Keyword(s):  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Rectal Biopsy

Diagnosing Hirschsprung's disease: Increasing the odds of a positive rectal biopsy result

2003 ◽  
Vol 38 (3) ◽  
pp. 412-416 ◽  
Author(s):  
Nicola A. Lewis ◽  
Marc A. Levitt ◽  
Garret S. Zallen ◽  
Mona S. Zafar ◽  
Karen L. Iacono ◽  
...  
Keyword(s):  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Rectal Biopsy ◽  
Biopsy Result

scholarly journals Hirschsprung’s Disease: Diagnosis and Management

2021 ◽  
Vol 10 (2) ◽  
pp. 104-113
Author(s):  
Md Benzamin ◽  
Md Rukunuzzaman ◽  
Md Wahiduzzaman Mazumder ◽  
ASM Bazlul Karim
Keyword(s):  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Rectal Biopsy ◽  
Disease Diagnosis ◽  
Radiological Investigation ◽  
Functional Intestinal Obstruction ◽  
The Common ◽  
High Index Of Suspicion ◽  
Hirschprung's Disease ◽  
Related Mortality

Hirschsprung’s disease (HD) is a rare genetic congenital defect of intestine causing failure of migration of parasympathetic ganglionic cells in some definite part of intestine, resulting in functional intestinal obstruction. It commonly involves rectosigmoid region of colon but other parts of colon or total colon, even small intestine may be affected. Incidence is 1/5000 live births. It is one of the common pediatric surgical problems and 2nd most organic cause of constipation. Symptoms may be evident from 1st day of life. About 90% infants with Hirschprung’s disease fail to pass meconium in 1st 24 hours of life. About 80% HD cases are diagnosed in early few months of life and present with abdominal distention, constipation, poor feeding, vomiting etc. HD enterocolitis is a devastating condition related to mortality. HD may be associated with some congenital anomalies and syndrome. High index of suspicion is the main key to diagnosis. Radiological investigation supports the diagnosis and rectal biopsy for histopathology is confirmatory. Although it is a surgical problem, physician can play a key role in early diagnosis and thus help to prevent HD enterocolitis-related mortality and restore near-normal life. J Enam Med Col 2020; 10(2): 104-113

Experience with full-thickness rectal biopsy in the evaluation of patients with suspected Hirschsprung’s disease

2014 ◽  
Vol 10 (2) ◽  
pp. 42-45
Author(s):  
Kayode T. Bamigbola ◽  
Abdulrasheed A. Nasir ◽  
Lukman O. Abdur-Rahman ◽  
Adewale O. Oyinloye ◽  
Nurudeen T. Abdulraheem ◽  
...  
Keyword(s):  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Rectal Biopsy ◽  
Full Thickness

Erythrocyte acetylcholinesterase activity in Hirschsprung's disease in Israel

1991 ◽  
Vol 26 (2) ◽  
pp. 190-191 ◽  
Author(s):  
O. Atias ◽  
R. Finaly ◽  
N. Meyerstein ◽  
A.J. Mares
Keyword(s):  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Acetylcholinesterase Activity

scholarly journals Full-thickness rectal biopsy in children suspicious for Hirschsprung's disease is safe and yields a low number of insufficient biopsies

2018 ◽  
Vol 53 (10) ◽  
pp. 1942-1944 ◽  
Author(s):  
Niels Bjørn ◽  
Lars Rasmussen ◽  
Niels Qvist ◽  
Sönke Detlefsen ◽  
Mark Bremholm Ellebæk
Keyword(s):  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Rectal Biopsy ◽  
Full Thickness

Studies of RET gene expression and acetylcholinesterase activity in a series of sporadic Hirschsprung’s disease

2008 ◽  
Vol 24 (9) ◽  
pp. 1017-1021 ◽  
Author(s):  
Maria Cecília M. Coelho ◽  
Uenis Tannuri ◽  
Israel Benditt ◽  
Maria Merces Santos
Keyword(s):  
Gene Expression ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Acetylcholinesterase Activity ◽  
Ret Gene
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