Gel-diffusion studies with mosquito (diptera, culicidae) antigens. I. Antigenic analysis during metamorphosis

Cowpox soluble antigen is shown by gel-diffusion studies to contain at least nine precipitable substances whose presence is induced by virus infection. One of these substances, pattern component d, is not produced in white cowpox infections, and its production in vaccinia infection can be inferred only from the presence of small amounts of anti-d in anti-vaccinia sera.Comparison of cowpox with vaccinia has led to the recognition of a further line pattern component f which is readily demonstrated as a soluble precipitable substance in simple buffer extracts of vaccinia-infected tissue, but is demonstrated in extracts of cowpox-infected tissue only with difficulty; anti-f antibody is readily detected in antisera prepared against cowpox, white cowpox, and vaccinia.

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Comparative antigenic analysis of pathogenic and free-living Naegleria species by the gel diffusion and immunoelectrophoresis techniques.

Infection and Immunity ◽

10.1128/iai.11.1.95-108.1975 ◽

1975 ◽

Vol 11 (1) ◽

pp. 95-108 ◽

Cited By ~ 11

Author(s):

G S Visvesvara ◽

G R Healy

Keyword(s):

Antigenic Analysis ◽

Free Living ◽

Gel Diffusion

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Gel Diffusion Studies on Serotype and Serotype Transformation in Paramecium

Journal of General Microbiology ◽

10.1099/00221287-21-1-156 ◽

1959 ◽

Vol 21 (1) ◽

pp. 156-167 ◽

Cited By ~ 9

Author(s):

E. BALBINDER ◽

J. R. PREER

Keyword(s):

Diffusion Studies ◽

Gel Diffusion

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Antigenic analysis of Shigella sonnei by gel diffusion technics

Canadian Journal of Microbiology ◽

10.1139/m68-122 ◽

1968 ◽

Vol 14 (6) ◽

pp. 737-743

Author(s):

W. J. Martin ◽

W. E. Mock ◽

W. H. Ewing

Keyword(s):

Shigella Sonnei ◽

Soluble Antigen ◽

Antigenic Relationship ◽

Antigenic Analysis ◽

Additional Information ◽

Shigella Boydii ◽

The Family ◽

Heterologous Antigens ◽

Gel Diffusion

The technics of immunogel diffusion as applied to soluble antigen concentrates of both smooth (S or DI) and rough (R or DII) forms of Shigella sonnei were studied. By using these procedures and using both homologous and heterologous antigens and antisera as well as specifically absorbed antisera, data were obtained that provided additional information which helped to clarify S–R (DI–DII) variations and the O and K antigens of S. sonnei. The same technics also were utilized to show the antigenic relationship between DI forms of S. sonnei and the C27 strain of Aeromonas shigelloides. The results of these studies suggested that O and possibly K antigens were shared between these two bacteria. Moreover, the association between the respective DII and R forms of S. sonnei and Shigella boydii 6 was confirmed and suggested the possibility of a degraded O antigen relationship. The applicability of gel diffusion methods by visual characterization of the antigenic interrelationships within members of the family Enterobacteriaceae was noted.

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