Agar gel diffusion studies of paravaccinia viruses

1968 ◽  
Vol 78 (2) ◽  
pp. 219-225 ◽  
Author(s):  
O.A. Papadopoulos ◽  
P.S. Dawson ◽  
R.A. Huck ◽  
P. Stuart
Keyword(s):  
Agar Gel ◽  
Diffusion Studies ◽  
Gel Diffusion

scholarly journals Serotyping reanalysis of unserotypable Actinobacillus pleuropneumoniae isolates by agar gel diffusion test

2016 ◽  
Vol 78 (4) ◽  
pp. 723-725 ◽  
Author(s):  
Ayako MORIOKA ◽  
Yoko SHIMAZAKI ◽  
Mariko UCHIYAMA ◽  
Shoko SUZUKI
Keyword(s):  
Actinobacillus Pleuropneumoniae ◽  
Diffusion Test ◽  
Agar Gel ◽  
Gel Diffusion

Pathogenic Vibrios in Confinement-Reared and Feral Fishes of the Maine–New Hampshire Coast

1978 ◽  
Vol 35 (4) ◽  
pp. 403-408 ◽  
Author(s):  
R. G. Strout ◽  
E. S. Sawyer ◽  
B. A. Coutermarsh
Keyword(s):  
New Hampshire ◽  
West Coast ◽  
Coho Salmon ◽  
Vibrio Anguillarum ◽  
Pseudopleuronectes Americanus ◽  
Agar Gel ◽  
Pathogenic Vibrio ◽  
Test Fish ◽  
Pathogenic Vibrios ◽  
Gel Diffusion

Vibrio anguillarum was isolated from moribund or fresh dead confinement-reared or feral fishes from the Maine–New Hampshire coast. All Vibrio isolates were tested for pathogenicity by inoculation into 12–15 cm-coho salmon (Oncorhynchus kisutch) smolts reared in fresh water. In 1975, of 35 isolates from confinement-reared fishes, 29 were Vibrio and 22 of these killed test fish; 1 of 4 isolates from feral fishes was a pathogenic Vibrio. In 1976, of 69 isolates from cultured fishes, 52 were Vibrio and 12 were pathogenic; 39 of 59 isolates from feral fishes were Vibrio, yet only 1 was pathogenic. One group of Vibrio isolates, all from winter flounder (Pseudopleuronectes americanus), killed all salmon smolts the same day of injection; one strain (569) consistently killed smolts within 3–4 h after inoculation. With microtiter methods, agglutinin titers of rabbit antisera were determined against all pathogenic V. anguillarum isolates. Three distinct antigenic groups of V. anguillarum, confirmed by agar-gel diffusion and challenge, were found on the Maine–New Hampshire coast. Although two of these groups appear to be antigenically similar to West Coast strains 775 and 1669, a third group shows little relationship to other East Coast–West Coast serotypes. Key words: Vibrio anguillarum, coho salmon, pathogenic Vibrio, confinement-reared (cultured) fishes, serotypes, agar-gel diffusion

scholarly journals THE IDENTIFICATION AND ISOLATION OF TWO MOUSE-TOXIC PROTEIN COMPONENTS IN EXTRACTS FROM PASTEURELLA PESTIS

1964 ◽  
Vol 120 (6) ◽  
pp. 1201-1213 ◽  
Author(s):  
Thomas C. Montie ◽  
Diane B. Montie ◽  
Samuel J. Ajl
Keyword(s):  
Disc Electrophoresis ◽  
Cytoplasmic Fraction ◽  
Agar Gel ◽  
Toxin B ◽  
Precipitin Reaction ◽  
Surface Active Agents ◽  
Surface Active ◽  
Protein Components ◽  
Gel Diffusion ◽  
Electrophoresis Technique

The toxin activity of Pasteurella pestis cells, strain "Tjiwidej," was found to be associated with two proteins. Using a disc electrophoresis technique in conjunction with mouse lethality, two toxic proteins were isolated exhibiting intraperitoneal LD50's of less than 1.0 to 1.5 µg protein. Each produced a single characteristic precipitin band on agar gel diffusion plates. The slower migrating toxin in gel diffusion or disc electrophoresis was designated as toxin A. It was shown to be sensitive to deoxycholate and digitonin, did not accumulate in 5-fluorotryptophan treated cells, and was associated with the membrane fraction of the cell. The faster migrating toxin B, apparently is resistant to surface-active agents, and is not affected by treatment of cells with 5-fluorotryptophan. Toxin B is associated with the soluble or cytoplasmic fraction of the cell. This evidence suggested that each toxin represented a distinctly different molecular species. The possibility is discussed that toxin B is synonymous with the murine toxin previously isolated by paper curtain electrophoresis which revealed only one antigen band in the Oudin precipitin reaction.

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