Trypanosoma brucei: Biochemical and morphological studies of cytotoxicity caused by normal human serum

1984 ◽  
Vol 58 (1) ◽  
pp. 81-93 ◽  
Author(s):  
M.R. Rifkin
Keyword(s):  
Human Serum ◽  
Trypanosoma Brucei ◽  
Normal Human Serum ◽  
Morphological Studies ◽  
Normal Human

scholarly journals The main lytic factor of Trypanosoma brucei brucei in normal human serum is not high density lipoprotein.

1996 ◽  
Vol 183 (3) ◽  
pp. 1023-1029 ◽  
Author(s):  
J Raper ◽  
V Nussenzweig ◽  
S Tomlinson
Keyword(s):  
High Density Lipoprotein ◽  
Human Serum ◽  
Trypanosoma Brucei ◽  
Gel Filtration ◽  
Density Lipoprotein ◽  
High Density ◽  
Lytic Activity ◽  
Normal Human Serum ◽  
Trypanosoma Brucei Brucei ◽  
Normal Human

Natural immunity of humans to the cattle pathogen Trypanosoma brucei brucei has been attributed to the presence in normal human serum (NHS) of lytic factors for the parasites. We and others have shown that NHS contains two trypanolytic factors (herein termed TLF1 and TLF2) that can be separated by gel filtration. TLF1 copurifies with a subclass of high density lipoprotein (HDL), whereas TLF2 has a much higher molecular weight and does not appear to be a lipoprotein. We find that the trypanolytic activity of purified TLF1 is totally inhibited by exogenous haptoglobin (Hp) at concentrations (0.1 mg/ml) lower than those present in NHS (0.2-2 mg/ml). In contrast, exogenous Hp (up to 2.5 mg/ml) has no effect on the lytic activity of either NHS or isolated TLF2. Hp-depleted sera from patients with intravascular hemolysis is severalfold more trypanolytic than NHS. These sera contain only TLF1, and their lytic activity is totally abolished upon the addition of Hp (0.1 mg/ml). When NHS containing different Hp allotypes is fractionated by gel filtration, TLF1 activity is either revealed or remains masked, depending on whether it coelutes with Hp. Masked TLF1 activity in the column fractions is revealed if Hp is removed by density gradient ultracentrifugation. We conclude that endogenous Hp inhibits TLF1 activity, and that TLF2 is the main trypanolytic factor in NHS.

Evaluation of a commercially available radioimmunoassay kit for measurement of doxorubicin in plasma.

1982 ◽  
Vol 28 (1) ◽  
pp. 119-121 ◽  
Author(s):  
E Piall ◽  
G W Aherne ◽  
V Marks
Keyword(s):  
Human Serum ◽  
Normal Human Serum ◽  
Nonspecific Effects ◽  
Normal Human ◽  
Doxorubicin Concentration

Abstract We evaluated a commercially available (Diagnostic Biochemistry Inc.) doxorubicin 125I radioimmunoassay kit. This kit gave a high apparent doxorubicin concentration (greater than 12 micrograms/L), which was not linearly related to dilution, for two pools of normal human serum and plasma and also for samples collected from patients before they received the drug. In contrast, a doxorubicin 3H radioimmunoassay developed by us gave a low blank (2 micrograms/L), which was linearly related to dilution, for the same pools and patients' samples. Doxorubicin concentrations in the plasma of patients receiving the drug were compared by the two methods; the kit gave results five- to 10-fold those obtained with our assay. High nonspecific interference by serum and plasma as measured by the 125I radioimmunoassay must therefore be borne in mind by users of the kit, and we suggest that results should be corrected for these nonspecific effects.

The Presence of Two Forms of Insulin in Normal Human Serum

Biochemistry ◽  
1963 ◽  
Vol 2 (2) ◽  
pp. 286-289 ◽  
Author(s):  
Walter N. Shaw ◽  
Eldon W. Shuey
Keyword(s):  
Human Serum ◽  
Normal Human Serum ◽  
Normal Human
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