Identification by flow cytometry of two distinct rhodamine-123-stained mitochondrial populations in rat liver

FEBS Letters ◽  
1989 ◽  
Vol 254 (1-2) ◽  
pp. 115-120 ◽  
Author(s):  
C. Lopez-Mediavilla ◽  
A. Orfao ◽  
M. Gonzalez ◽  
J.M. Medina
Keyword(s):  
Flow Cytometry ◽  
Rat Liver ◽  
Rhodamine 123

scholarly journals Intravital multiphoton imaging of rhodamine 123 in the rat liver after intravenous dosing

IntraVital ◽  
2012 ◽  
Vol 1 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Xin Liu ◽  
Camilla A. Thorling ◽  
Lu Jin ◽  
Michael S. Roberts
Keyword(s):  
Rat Liver ◽  
Rhodamine 123 ◽  
Multiphoton Imaging

scholarly journals Changes in adult rat liver mitochondrial populations at different energy states analyzed by flow cytometry

1995 ◽  
Vol 1232 (1-2) ◽  
pp. 27-32 ◽  
Author(s):  
Casilda López-Mediavilla ◽  
Alberto Orfao ◽  
M. Victoria García ◽  
JoséM. Medina
Keyword(s):  
Flow Cytometry ◽  
Rat Liver ◽  
Adult Rat ◽  
Energy States

scholarly journals Effects and Mechanism of Oxymatrine Combined with Compound Yinchen Granules on the Apoptosis of Hepatocytes through the Akt/FoxO3a/Bim Pathway

2022 ◽  
Vol 2022 ◽  
pp. 1-11
Author(s):  
Xian Zhang ◽  
Jiajia Ge ◽  
Xuejuan Zhu ◽  
Haifeng Zhang ◽  
Yuanzi Wang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Western Blot ◽  
Liver Failure ◽  
Acute Liver Failure ◽  
Rat Liver ◽  
Caspase 3 ◽  
Normal Group ◽  
Model Group ◽  
Apoptosis Rate ◽  
Liver Tissues

The aim of the present study was to investigate the effects and mechanism of oxymatrine (OMT) combined with compound yinchen granules (CYG) on the apoptosis of hepatocytes through the Akt/FoxO3a/Bim pathway in rats with acute liver failure. The rat model of acute liver failure was established using lipopolysaccharide/D-galactosamine (LPS/D-GalN). The expression of proteins in rat liver tissues was detected by western blot analysis. The mRNA expression of FoxO3a, Bim, Bax, Bcl-2, and caspase-3 in rat liver tissues was detected by RT-qPCR. The apoptosis rate of rat hepatocytes was determined by flow cytometry. Western blots showed that when compared with the normal group, the expression of p-Akt and p-FoxO3a in the model group was decreased ( P < 0.05 ), while the expression of Bim was increased ( P < 0.01 ). Compared with the model group, the expression of p-Akt and p-FoxO3a in the OMT group and the OMT combined with CYG groups was increased ( P < 0.05 or P < 0.01 ), while the expression of Bim was decreased ( P < 0.05 ). The Bax/Bcl-2 ratio and caspase-3 protein expression in the model group were significantly higher than those in the normal group ( P < 0.01 ). The Bax/Bcl-2 ratio and the expression of caspase-3 protein in the OMT group and the OMT combined with CYG groups were significantly lower than those in the model group ( P < 0.01 ). The results of RT-qPCR were consistent with those of western blot. The results of flow cytometry showed that the apoptosis rate of hepatocytes in the OMT group and the OMT combined with CYG groups was significantly lower than that in the model group ( P < 0.05 or P < 0.01 ). We concluded that LPS/D-GalN can induce apoptosis of hepatocytes in rats with acute liver failure through the Akt/FoxO3a/Bim pathway. OMT combined with CYG inhibits apoptosis of hepatocytes in rats with acute liver failure via the Akt/FoxO3a/Bim pathway.

Rhodamine 123 inhibits import of rat liver mitochondrial transhydrogenase

1987 ◽  
Vol 144 (1) ◽  
pp. 477-483 ◽  
Author(s):  
Ira M. Lubin ◽  
Licia N.Y. Wu ◽  
Roy E. Wuthier ◽  
Ronald R. Fisher
Keyword(s):  
Rat Liver ◽  
Rhodamine 123

Developmental changes in rat liver mitochondrial populations analyzed by flow cytometry

1992 ◽  
Vol 203 (1) ◽  
pp. 134-140 ◽  
Author(s):  
C. López-Mediavilla ◽  
A. Orfao ◽  
J. San Miguel ◽  
J.M. Medina
Keyword(s):  
Flow Cytometry ◽  
Rat Liver ◽  
Developmental Changes

scholarly journals A fast kinetic method for assessing mitochondrial membrane potential in isolated hepatocytes with rhodamine 123 and flow cytometry

Cytometry ◽  
1994 ◽  
Vol 15 (4) ◽  
pp. 335-342 ◽  
Author(s):  
Gloria Juan ◽  
Marika Cavazzoni ◽  
Guillermo T. Sáez ◽  
José-Enrique O'Connor
Keyword(s):  
Flow Cytometry ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Kinetic Method ◽  
Isolated Hepatocytes ◽  
Rhodamine 123 ◽  
Fast Kinetic

Rhodamine 123 inhibits bioenergetic function in isolated rat liver mitochondria

1984 ◽  
Vol 118 (3) ◽  
pp. 717-723 ◽  
Author(s):  
Josephine S. Modica-Napolitano ◽  
Michael J. Weiss ◽  
Lan Bo Chen ◽  
June R. Aprille
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Rhodamine 123 ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Isolated Rat

Effect of Thyroid Hormone on the Ploidy of Rat Liver Nuclei as Determined by Flow-Cytometry

1978 ◽  
Vol 158 (1) ◽  
pp. 63-67 ◽  
Author(s):  
J. Mendecki ◽  
W. H. Dillmann ◽  
R. C. Wolley ◽  
J. H. Oppenheimer ◽  
L. G. Koss
Keyword(s):  
Flow Cytometry ◽  
Thyroid Hormone ◽  
Rat Liver ◽  
Rat Liver Nuclei ◽  
Liver Nuclei

Effect of Ethanol Consumption on Adult Rat Liver Mitochondrial Populations Analyzed by Flow Cytometry

1995 ◽  
Vol 19 (5) ◽  
pp. 1327-1330 ◽  
Author(s):  
Antonio Mateos ◽  
Alberto Orfao ◽  
Angeles Almeida ◽  
Maria Isabel Martin ◽  
Casilda Lopez-Mediavilla ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Rat Liver ◽  
Ethanol Consumption ◽  
Adult Rat
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