Isolation of granules containing adipokinetic hormone from locust corpora cardiaca by differential centrifugation

1979 ◽  
Vol 39 (4) ◽  
pp. 543-547 ◽  
Author(s):  
Judith V. Stone ◽  
William Mordue
1990 ◽  
Vol 269 (2) ◽  
pp. 309-313 ◽  
Author(s):  
G Gäde ◽  
H Wilps ◽  
R Kellner

A hypertrehalosaemic neuropeptide from the corpora cardiaca of the blowfly Phormia terraenovae has been isolated by reversed-phase h.p.l.c., and its primary structure was determined by pulsed-liquid phase sequencing employing Edman chemistry after enzymically deblocking the N-terminal pyroglutamate residue. The C-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase A. The octapeptide has the sequence pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 and is clearly defined as a novel member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of peptides. It is the first charged member of this family to be found. The synthetic peptide causes an increase in the haemolymph carbohydrate concentration in a dose-dependent fashion in blowflies and therefore is named ‘Phormia terraenovae hypertrehalosaemic hormone’ (Pht-HrTH). In addition, receptors in the fat-body of the American cockroach (Periplaneta americana) recognize the peptide, resulting in carbohydrate elevation in the blood. However, fat-body receptors of the migratory locust (Locusta migratoria) do not recognize this charged molecule, and thus no lipid mobilization is observed in this species.


1995 ◽  
Vol 57 (3) ◽  
pp. 297-310 ◽  
Author(s):  
Dick R. Nässel ◽  
Paul C.C.M. Passier ◽  
Karoly Elekes ◽  
Heinrich Dircksen ◽  
Henk G.B. Vullings ◽  
...  

2000 ◽  
Vol 267 (17) ◽  
pp. 5502-5508 ◽  
Author(s):  
Gabriele V. Köllisch ◽  
Matthias W. Lorenz ◽  
Roland Kellner ◽  
Peter D. Verhaert ◽  
Klaus H. Hoffmann

1996 ◽  
Vol 21 (3) ◽  
pp. 167-172 ◽  
Author(s):  
A. AYALI ◽  
M.P. PENER ◽  
S.M. SOWA ◽  
L.L. KEELEY

1995 ◽  
Vol 97 (2) ◽  
pp. 231-238 ◽  
Author(s):  
P.C.C.M. Passier ◽  
H.G.B. Vullings ◽  
J.H.B. Diederen ◽  
D.J. Van der Horst

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