Metamorphic degeneration of intersegmental muscles in cabbage white butterfly, Pieris brassicae L. (Lepidoptera: Pieridae)

1979 ◽  
Vol 8 (5-6) ◽  
pp. 387-393
Author(s):  
Thota Srihari
Author(s):  
Sonika Sharma ◽  
Hafeez Ahmad ◽  
Suheel Ahmad Ganai ◽  
Devinder Sharma ◽  
Thanlass Norboo ◽  
...  

2017 ◽  
Vol 108 (4) ◽  
pp. 501-509
Author(s):  
A. Sharifloo ◽  
A. Zibaee ◽  
J. Jalali Sendi ◽  
K. Talebi Jahroumi

AbstractA comprehensive study on digestive trypsin was undertaken in the larval midgut of Pieris brassicae L. Results of enzymatic compartmentalization showed a significantly higher activity of crude trypsin in the anterior larval midgut rather than posterior-midgut. Using Diethylaminoethyl cellulose fast flow column chromatography a purified trypsin was obtained by specific activity of 21 U mg−1 protein, recovery of 22%, purification fold of 28-fold and molecular weight of 25 kDa. This purified enzyme showed the highest activity at pH 8 and the corresponding temperature of 40°C. However, the specific inhibitors used including 4-(2-Aminoethyl) benzenesulfonyl fluroride hydrochloride, N-p-Tosyl-L-lysine methyl ester hydrochloride and Soybean Trypsin Inhibitor significantly lowered the activity of the purified enzyme in vitro. Moreover, the activity of trypsin and likewise the nutritional indices were significantly altered in the larval midgut feeding upon the leaves treated by 1 mM concentration of each inhibitor in comparison with control. Determination of enzymatic characteristics of insect trypsins is crucial in paving the path for controlling pests by potential natural compounds via transgenic plants.


PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e59661 ◽  
Author(s):  
Sven Geiselhardt ◽  
Kinuyo Yoneya ◽  
Beatrice Blenn ◽  
Navina Drechsler ◽  
Jonathan Gershenzon ◽  
...  

1990 ◽  
Vol 270 (1) ◽  
pp. 133-136 ◽  
Author(s):  
N Crickmore ◽  
C Nicholls ◽  
D J Earp ◽  
T C Hodgman ◽  
D J Ellar

Using our recently reported method of electroporation to transform Bacillus thuringiensis [Bone & Ellar (1989) FEMS Microbiol. Lett. 58, 171-178], cloned B. thuringiensis entomocidal delta-endotoxin genes have been introduced into several native B. thuringiensis strains. In many cases the resulting transformants expressed both their native toxins and the cloned toxin, producing strains with broader toxicity spectra. The introduction of the var. tenebrionis toxin gene into B. thuringiensis var. israelensis resulted in a strain with activity against Pieris brassicae (cabbage white butterfly), an activity which neither parent strain possesses. We discuss further the possibility of synergism and also the problems associated with introducing cloned DNA by this method.


2006 ◽  
Vol 56 (2) ◽  
pp. 157-172 ◽  
Author(s):  
Renate Smallegange ◽  
Tjarda Everaarts ◽  
Joop Van Loon

AbstractThe landing response of the large cabbage white butterfly Pieris brassicae was studied under controlled optical and gustatory stimulus conditions. Experience-based changes in landing behaviour were examined by offering cardboard circles of two different shades of green, treated with either an oviposition stimulant or a deterrent. We employed two training situations. In one situation the two shades of green, carrying either the stimulant or the deterrent, were offered simultaneously, in the other sequentially. During the 1 hour training periods, butterflies were either landing and drumming spontaneously or they were caught at the end of the period and placed on the artificial leaves until tarsal drumming ensued. Our experiments demonstrated that P. brassicae females can learn to associate visually detected substrate characteristics with contact-chemosensory information available only after landing. Furthermore, a learned preference for a substrate could be turned into a preference for the alternative substrate by exposing the insect to a deterrent on the previously preferred substrate. These results provide indications of aversion learning, thus far undocumented in oviposition behaviour of Lepidoptera. Bringing the butterflies into forced contact with the oviposition stimulant resulted in similar effects on landing preference compared to those of spontaneous landing, but spontaneous landing had a stronger effect on preference for associations involving the deterrent. The simultaneous training regime, which supposedly requires a less important role for short-term memory, was more effective in modifying landing preferences.


2021 ◽  
Vol 09 (01) ◽  
pp. 44-48
Author(s):  
Aneela Kanwal ◽  
Mehboob Ahmad ◽  
Isma Khurshid ◽  
Muhammad Pervaiz Khan ◽  
Shitab Khan ◽  
...  

2014 ◽  
Vol 5 (2) ◽  
pp. 128
Author(s):  
Hamid Ullah ◽  
Ibrar Muhammad ◽  
Waheed Ullah ◽  
Farzana Parveen ◽  
Sohail Aslam

Tea plants are visited by different types of insects including tea pests and pollinator. The objective of the study was to identify tea associated insects in National Tea Research Institute (NTRI), Mansehra, Pakistan during April-June 2012. A collection of tea associated insects was made during April-June 2012 in Tea Experimental Garden (TEG), NTRI, Mansehra, Pakistan. A total of 128 samples were collected from TEG randomly. During the present research, 10 species were identified. Species collected were brush-footed butterfly, Argynnis hyperbius (Linnaeus, 1763); painted lady, Vanessa cardui (Linnaeus, 1758), Lucerne butterfly, Colias electo (Linnaeus, 1763)( Nymphalidae); small cabbage white butterfly, Pieris rapae (Linnaeus, 1758), white cabbage butterfly, Pieris brassicae (Linnaeus, 1758)( Pieridae); Asiatic honey bee, Apis cerana Fabricius, 1793, Western honey bee, Apis mellifera Linnaeus, 1758(Apidae); seven-spot ladybird Coccinella septempunctata (Linnaeus, 1758)( Coccinellidae); blue marsh hawk Orthetrum glaucum (Brauer, 1865), Crimson-tailed marsh hawk, Orthetrum pruinosum neglectum (Rambur, 1842)( Libellulidae). Different numbers of individuals were collected of each species. Seventeen individuals of P. brassicae, 16 of C. septempunctata, 15 of P. rapae, 14 of C. electo, 13 of V. cardui and A. cerana, 10 of A. mellifera, 9 of O. pruinosum neglectum and 7 of O. glaucum.It is concluded that there is a diversity to explain tea associated insects in NTRI, Mansehra, Pakistan.


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