Scale-up of recombinant protein purification by hydrophobic interaction chromatography

1992 ◽  
Vol 625 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Karola Vorauer ◽  
Marlies Skias ◽  
Alexandra Trkola ◽  
Petra Schulz ◽  
Alois Jungbauer
Keyword(s):  
Recombinant Protein ◽  
Protein Purification ◽  
Hydrophobic Interaction ◽  
Scale Up ◽  
Hydrophobic Interaction Chromatography ◽  
Recombinant Protein Purification

Recombinant protein purification from pea

2004 ◽  
Vol 86 (1) ◽  
pp. 108-114 ◽  
Author(s):  
Todd J. Menkhaus ◽  
Cynthia Pate ◽  
Anthony Krech ◽  
Charles E. Glatz
Keyword(s):  
Recombinant Protein ◽  
Protein Purification ◽  
Recombinant Protein Purification

scholarly journals A Simple Method for the Estimation of Protein Retention in Hydrophobic Interaction Chromatography Under Different Operation Conditions

2007 ◽  
Vol 1 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Andrea Mahn ◽  
M. Elena Lienqueo ◽  
Juan A. Asenjo
Keyword(s):  
Hydrophobic Interaction ◽  
Linear Correlation ◽  
Retention Time ◽  
Process Design ◽  
Scale Up ◽  
Hydrophobic Interaction Chromatography ◽  
Simple Method ◽  
Protein Retention ◽  
Operation Conditions

Protein behavior in Hydrophobic Interaction Chromatography using different chromatographic conditions was investigated. A linear correlation was found between protein retention time on different matrixes and different initial elution salt concentrations. Mathematical correlations between retention times under different chromatographic conditions were obtained and validated, which can be used in process design and scale-up.

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