Characterization of calmodulin-dependent and cyclic AMP-dependent protein kinase stimulation of cardiac sarcoplasmic reticulum calcium transportS. Katz, B. Richter and B. Eibschutz. Division of Pharmacology, Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, V6T 1W5, Canada

1983 ◽  
Vol 15 ◽  
pp. 87-87
Keyword(s):  
Protein Kinase ◽  
Sarcoplasmic Reticulum ◽  
Pharmaceutical Sciences ◽  
Dependent Protein Kinase ◽  
Cardiac Sarcoplasmic Reticulum ◽  
University Of British Columbia ◽  
Dependent Protein ◽  
Sarcoplasmic Reticulum Calcium ◽  
Stimulation Of

scholarly journals Translation of Ser16 and Thr17 phosphorylation of phospholamban into Ca2+-pump stimulation

1996 ◽  
Vol 316 (1) ◽  
pp. 201-207 ◽  
Author(s):  
Wayne A. JACKSON ◽  
John COLYER
Keyword(s):  
Protein Kinase ◽  
Sarcoplasmic Reticulum ◽  
Dependent Protein Kinase ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Oligomeric Protein ◽  
Pump Activity ◽  
Dependent Protein ◽  
Indicator Dye ◽  
Phospholamban Phosphorylation ◽  
Stimulation Of

Stimulation of cardiac sarcoplasmic reticulum Ca2+-pump activity is achieved by phosphorylation of the oligomeric protein phospholamban at either Ser16 or Thr17. The altered mobility of phosphorylated forms of pentameric phospholamban has been utilized to demonstrate that the mechanisms of phosphorylation of the two sites differ. Phosphorylation of Ser16 by the AMP-dependent protein kinase proceeds via a random mechanism [Li, Wang and Colyer (1990) Biochemistry 29, 4535–4540], whereas phosphorylation of Thr17 by calmodulin-dependent protein kinase is shown here to proceed via a co-operative mechanism. This co-operative reaction mechanism was unaffected by the phosphorylation status of Ser16. These two mechanisms of phosphorylation generate very different phosphoprotein profiles depending on whether the Ser16 or Thr17 residue is phosphorylated. The translation of these patterns of phosphorylation into Ca2+-pump function was reviewed using a fluorimetric Ca2+-indicator dye, fluo-3, to measure Ca2+ uptake by cardiac sarcoplasmic reticulum vesicles. The rate of Ca2+ accumulation, which parallels Ca2+-pump activity, was stimulated in proportion with the stoichiometry of phospholamban phosphorylation, irrespective of whether phosphorylation was on Ser16 or Thr17.

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