In vivo incorporation of methyl groups into the ribose of Escherichia coli ribosomal RNA

1967 ◽  
Vol 30 (3) ◽  
pp. 477-489 ◽  
Author(s):  
J.L. Nichols ◽  
B.G. Lane
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Methyl Groups

Investigation of the tertiary folding of Escherichia coli ribosomal RNA by intra-RNA cross-linking in vivo

1986 ◽  
Vol 191 (1) ◽  
pp. 135-138 ◽  
Author(s):  
Wolfgang Stiege ◽  
Johannes Atmadja ◽  
Monica Zobawa ◽  
Richard Brimacombe
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Cross Linking ◽  
Tertiary Folding

scholarly journals Three Ribosomal Operons of Escherichia coli Contain Genes Encoding Small RNAs That Interact With Hfq and CsrA in vitro

2021 ◽  
Vol 12 ◽  
Author(s):  
Thomas Søndergaard Stenum ◽  
Mette Kongstad ◽  
Erik Holmqvist ◽  
Birgitte Kallipolitis ◽  
Sine Lo Svenningsen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Small Rnas ◽  
Genes Encoding ◽  
Ribosomal Operons ◽  
Dependent Processes ◽  
Fine Tune

Three out of the seven ribosomal RNA operons in Escherichia coli end in dual terminator structures. Between the two terminators of each operon is a short sequence that we report here to be an sRNA gene, transcribed as part of the ribosomal RNA primary transcript by read-through of the first terminator. The sRNA genes (rrA, rrB and rrF) from the three operons (rrnA, rrnB and rrnD) are more than 98% identical, and pull-down experiments show that their transcripts interact with Hfq and CsrA. Deletion of rrA, B, F, as well as overexpression of rrB, only modestly affect known CsrA-regulated phenotypes like biofilm formation, pgaA translation and glgC translation, and the role of the sRNAs in vivo may not yet be fully understood. Since RrA, B, F are short-lived and transcribed along with the ribosomal RNA components, their concentration reflect growth-rate regulation at the ribosomal RNA promoters and they could function to fine-tune other growth-phase-dependent processes in the cell. The primary and secondary structure of these small RNAs are conserved among species belonging to different genera of Enterobacteriales.

A mutation in an Escherichia coli ribosomal RNA operon that blocks the production of precursor 23 S ribosomal RNA by RNase III in vivo and in vitro

1985 ◽  
Vol 182 (2) ◽  
pp. 205-216 ◽  
Author(s):  
Michael J.R. Stark ◽  
Richard L. Gourse ◽  
David K. Jemiolo ◽  
Albert E. Dahlberg
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Rnase Iii
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