Nuclear RNA precursors in the processing pathway to MOPC 21 κ light chain messenger RNA

1979 ◽  
Vol 135 (4) ◽  
pp. 879-891 ◽  
Author(s):  
Maureen Gilmore-Hebert ◽  
Randolph Wall
Keyword(s):  
Light Chain ◽  
Messenger Rna ◽  
Nuclear Rna

Sequence analysis of immunoglobulin light chain messenger RNA

Nature ◽  
1974 ◽  
Vol 252 (5482) ◽  
pp. 354-359 ◽  
Author(s):  
C. Milstein ◽  
G. G. Brownlee ◽  
E. M. Cartwright ◽  
J. M. Jarvis ◽  
N. J. Proudfoot
Keyword(s):  
Sequence Analysis ◽  
Light Chain ◽  
Messenger Rna ◽  
Immunoglobulin Light Chain

scholarly journals Large heterogeneous nuclear ribonucleic acid has three times as many 5' caps as polyadenylic acid segments, and most caps do not enter polyribosomes.

1981 ◽  
Vol 1 (2) ◽  
pp. 179-187 ◽  
Author(s):  
M Salditt-Georgieff ◽  
M M Harpold ◽  
M C Wilson ◽  
J E Darnell
Keyword(s):  
Ribonucleic Acid ◽  
Messenger Rna ◽  
Turnover Rate ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Rna Molecules ◽  
Rapid Turnover ◽  
Polyadenylic Acid ◽  
Nuclear Rna ◽  
Unstable Molecules

The rate of synthesis in Chinese hamster cells of 5' cap structures, m7 GpppNmp, in large (greater than 700 bases) heterogeneous nuclear ribonucleic acid (RNA) molecules is two to three times faster than the synthesis of 3'-terminal polyadenylic acid segments. As judged by presence of caps, newly synthesized polysomal messenger RNA, exclusive of messenger RNA the size of histone messenger RNA, is more than 90% in the polyadenylated category. It appears, therefore, that between half and two-thirds of the long capped heterogeneous nuclear RNA molecules do not contribute a capped polysomal derivative to the cytoplasm. There are capped, nonpolysomal, non-polyadenylated molecules with a rapid turnover rate that fractionate with the cytoplasm. These metabolically unstable molecules either could represent leakage into the cytoplasm during fractionation or could truly spend a brief time in the cytoplasm before decay.

scholarly journals The export of immunoglobulin D by human neoplastic B lymphocytes.

1983 ◽  
Vol 157 (1) ◽  
pp. 337-341 ◽  
Author(s):  
F K Stevenson ◽  
G T Stevenson ◽  
A L Tutt
Keyword(s):  
B Lymphocytes ◽  
Light Chain ◽  
Messenger Rna ◽  
Secretory Pathway ◽  
Control Mechanism ◽  
Surface Membrane ◽  
Lambda Light Chain ◽  
Culture Cells ◽  
Light Chain Type ◽  
Chain Type

An investigation has been made into the ability of human neoplastic B lymphocytes expressing surface IgM and IgD to export IgD in culture. Cells that expressed surface Ig of the lambda light chain type frequently exported IgD (10/12 patients), whereas cells expressing surface Ig of the kappa light chain type exported no IgD, although most (8/11 patients) were able to export IgM. It appears, therefore, that in most of the 23 cases studied, cells synthesizing IgD with lambda light chains can both express and export IgD, whereas those synthesizing IgD kappa can only insert it into the surface membrane. This finding and the known preponderance of lambda in plasma IgD imply that the possession of a lambda chain facilitates the IgD secretory pathway, a conclusion that implicates a control mechanism subsequent to the surface/secretory dichotomy arising from different splicings of heavy chain messenger RNA.

scholarly journals Nuclear RNA-protein interactions and messenger RNA processing.

1983 ◽  
Vol 97 (5) ◽  
pp. 1321-1326 ◽  
Author(s):  
T Pederson
Keyword(s):  
Detailed Analysis ◽  
Recent Work ◽  
Protein Interactions ◽  
Rna Processing ◽  
Messenger Rna ◽  
Mammalian Cells ◽  
General Hypothesis ◽  
Ribonucleoprotein Particles ◽  
Nuclear Rna ◽  
Nuclear Ribonucleoprotein

Eucaryotic messenger RNA precursors are processed in nuclear ribonucleoprotein particles (hnRNP). Here recent work on the structure of hnRNP is reviewed, with emphasis on function. Detailed analysis of a specific case, the altered assembly of hnRNP in heat-shocked Drosophila and mammalian cells, leads to a general hypothesis linking hnRNP structure and messenger RNA processing.

II. Distribution of DNA base sequences - Introductory remarks: DNA and genes

1978 ◽  
Vol 283 (997) ◽  
pp. 305-307
Keyword(s):  
Genetic Code ◽  
Messenger Rna ◽  
Transfer Rnas ◽  
Rna Molecules ◽  
Dna Base ◽  
Base Sequences ◽  
Nuclear Rna

After the genetic code was discovered in the early 1960s, it was generally accepted that nearly all DNA in higher organisms was used to specify messenger RNA molecules at some time during their development. A small fraction could be set aside for the ribosomal and transfer RNAs and there was a problem about the rapidly turning over nuclear RNA which did not appear in the cytoplasm as message. By and large we considered that most DNA was potentially coding and the lone voices who talked of other kinds of DNA on the basis of somewhat flimsy evidence were largely ignored.

Ordered recombination of immunoglobulin light chain genes occurs at the IGK locus but seems less strict at theIGL locus

Blood ◽  
2001 ◽  
Vol 97 (4) ◽  
pp. 1001-1008 ◽  
Author(s):  
Mirjam van der Burg ◽  
Talip Tümkaya ◽  
Marjan Boerma ◽  
Sandra de Bruin-Versteeg ◽  
Anton W. Langerak ◽  
...  
Keyword(s):  
Light Chain ◽  
Messenger Rna ◽  
Somatic Mutations ◽  
Cell Model ◽  
Complete Analysis ◽  
Immunoglobulin Light Chain ◽  
Main Determinant ◽  
Heavy Chains ◽  
Single Cell Model ◽  
Rearrangement Processes

Abstract Regulation of allelic and isotypic exclusion of human immunoglobulin (Ig) light-chain genes was studied in 113 chronic B-cell leukemias as a “single-cell” model that allowed complete analysis of each light chain allele. Our data show that monospecific Ig light chain expression is in about 90% of cases determined by ordered recombination: Igκ gene (IGK) rearrangements, followed byIGK deletions and Igλ gene (IGL) rearrangements, resulting in the presence of only one functional Ig light chain rearrangement. In about 10% (10 cases), 2 functional Ig light chain rearrangements (IGK/IGL or IGL/IGL, but not IGK/IGK) were identified. This might be explained by the fact that regulation of the ordered recombination process is not fully strict, particularly when the IGL locus is involved. Unfavorable somatic mutations followed by receptor editing might have contributed to this finding. Eight of these 10 cases indeed contained somatic mutations. In cases with 2 functional Ig light chain rearrangements, both alleles were transcribed, but monospecific Ig expression was still maintained. This suggests that in these cases allelelic exclusion is not regulated at the messenger RNA level but either at the level of translation or protein stability or via preferential pairing of Ig light and Ig heavy chains. Nevertheless, ordered rearrangement processes are the main determinant for monospecific Ig light chain expression.

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