Extracellular magnesium decreases the secretory response of rat peritoneal mast cells to compound 48/80 in vitro

Life Sciences ◽  
1991 ◽  
Vol 49 (23) ◽  
pp. 1689-1697 ◽  
Author(s):  
Henrik Bertelsen ◽  
Torben Johansen
Keyword(s):  
Mast Cells ◽  
Secretory Response ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

scholarly journals DETERMINATION OF l-HISTIDINE IN NANOGRAM AMOUNTS AND THE QUESTION OF ITS OCCURRENCE IN MAST CELLS

1972 ◽  
Vol 20 (11) ◽  
pp. 917-922 ◽  
Author(s):  
DAVID I. WILKINSON ◽  
DAVID GLICK
Keyword(s):  
Mast Cells ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Peritoneal Mast Cells ◽  
Before And After ◽  
Chromatographic Detection ◽  
Rat Peritoneal Mast Cells ◽  
Rate Limiting

In an attempt to clarify the question of whether histidine is stored in the mast cell for coversion to histamine or whether the rate of conversion is rapid enough to prevent accumulation of histidine so that the rate-limiting step is the histidine uptake, it was found that no histidine was demonstrable in rat peritoneal mast cells by either quantitative analysis or paper chromatographic detection. Microadaptation of Hassall's method, based on conversion of l-histidine by histidase to urocanic acid and measurement of the latter by its absorbance at 277 nm, was made to permit determination of histidine in nanogram amounts in the presence of histamine. This adaptation was found reliable when compared with the o-phthalaldehyde method in estimation of l-histidine in serum and in insulin hydrolysate, and then it was applied to analysis of mast cells before and after l-histidine uptake in vitro. The adaptation should be generally useful in microanalysis of l-histidine in histologically and cytologically defined samples.

Effect of polyethyleneglycols (PEG 600 and PEG 6000) and bovine serum albumin on myeloperoxidase-mediated release of [3H]-serotonin from rat peritoneal mast cells in vitro

1984 ◽  
Vol 4 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Karl-Eric Magnusson ◽  
Olle Stendahl
Keyword(s):  
Mast Cells ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Peg 6000 ◽  
Peritoneal Mast Cells ◽  
Inflammatory Site ◽  
Rat Peritoneal Mast Cells ◽  
Peg 600

Rat peritoneal mast cells in vitro released [3H]-serotonin in the presence of halide (I-), myeloperoxidase (MPO), and a H2O2-generating system. The degranulation was partly inhibited with low concentrations of bovine serum albumin (BSA; 0.1–l% w/v), or polyethyleneglycols (PEG 600 or PEG 6000, 0.1% w/v). Furthermore, PEG 600 and PEG 6000 enhanced the stabilization achieved with 0.1% BSA. The results may provide a new aspect on antigenicity of PEG-modified allergens, and on mast-cell response at an inflammatory site.

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