Mapping temperature-sensitive mutants of simian virus 40: Rescue of mutants by fragments of viral DNA

Virology ◽  
1974 ◽  
Vol 60 (2) ◽  
pp. 466-475 ◽  
Author(s):  
Ching-Juh Lai ◽  
Daniel Nathans
Keyword(s):  
Simian Virus 40 ◽  
Simian Virus ◽  
Temperature Sensitive ◽  
Viral Dna ◽  
Temperature Sensitive Mutants

Transformation of Human Cells by Temperature-Sensitive Mutants of Simian Virus 40

Intervirology ◽  
1978 ◽  
Vol 9 (1) ◽  
pp. 28-38 ◽  
Author(s):  
Christopher A. Lomax ◽  
Edward Bradley ◽  
Joseph Weber ◽  
Pierre Bourgaux
Keyword(s):  
Simian Virus 40 ◽  
Simian Virus ◽  
Human Cells ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants

Temperature-Sensitive Mutants of Simian Virus 40

Intervirology ◽  
1978 ◽  
Vol 10 (3) ◽  
pp. 181-195 ◽  
Author(s):  
Christine A. Noonan ◽  
Janet S. Butel
Keyword(s):  
Simian Virus 40 ◽  
Simian Virus ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants

scholarly journals Rat cells transformed by simian virus 40 give rise to tumor cells which contain no viral proteins and often no viral DNA.

1983 ◽  
Vol 3 (6) ◽  
pp. 1138-1145 ◽  
Author(s):  
R Seif ◽  
I Seif ◽  
J Wantyghem
Keyword(s):  
Tumor Cells ◽  
Viral Genome ◽  
Simian Virus 40 ◽  
Viral Proteins ◽  
Simian Virus ◽  
Tumor Formation ◽  
Temperature Sensitive ◽  
3T3 Cells ◽  
Viral Dna ◽  
Transformed Cell Lines

Rat 3T3 cells transformed by simian virus 40 were injected into rats to examine their capacity to develop into tumors. Both large T-dependent (N) transformants and large T-independent (A) transformants were used. All the transformed cell lines contained large T and small t and could multiply efficiently in agar. Only some transformants could develop into tumors. All tumor cells examined had lost both large T and small t. Tumor cells in which the viral genome could still be detected were found together with tumor cells in which the simian virus 40 DNA could no longer be detected. N transformants which displayed the transformed phenotype in a temperature-sensitive manner became temperature insensitive during tumor formation.

Sign in / Sign up

Export Citation Format

Share Document