Capsid-DNA complexes in the DNA packaging pathway of bacteriophage T7: Characterization of the capsids bound to monomeric and concatemeric DNA

Virology ◽  
1981 ◽  
Vol 108 (1) ◽  
pp. 164-176 ◽  
Author(s):  
Philip Serwer ◽  
Robert H. Watson
Keyword(s):  
Dna Packaging ◽  
Bacteriophage T7 ◽  
Dna Complexes

Observation of Fast Sedimenting, Replicating Bacteriophage T7 DNA by Negative Staining

1980 ◽  
Vol 38 ◽  
pp. 538-539
Author(s):  
P. Serwer
Keyword(s):  
Electron Microscopy ◽  
Cytochrome C ◽  
Dna Packaging ◽  
Negative Staining ◽  
Bacteriophage T7 ◽  
Dna Complexes ◽  
Sufficient Detail ◽  
Protein Monolayer

To package the DNA of bacteriophage T7, a preformed, DNA-free capsid (capsid I) with an envelope thicker than the envelope of bacteriophage T7:(a) binds DNA, (b) converts to a capsid (capsid II) with a bacteriophage-like envelope prior to packaging DNA and (c) draws in DNA (1). During attempts to understand T7 DNA packaging, complexes of capsids with mature T7 DNA and complexes of capsids with longer than mature, linear T7 DNA have been isolated (2). Objects with capsid-like dimensions were observed on a fast sedimenting, replicating complex of T7 DNA (100S+ DNA) prepared for electron microscopy using a protein monolayer-shadowing technique (3). This procedure for preparation of specimens does not, however, reveal sufficient detail to rigorously identify an object as a capsid. To better visualize objects bound to 100S+ T7 DNA, this DNA has been prepared for electron microscopy using the aqueous technique for the negative staining of capsid-DNA complexes more recently described (4) (the DNA is coated with cytochrome c and is revealed in an extended configuration).

Characterization of Heme–DNA Complexes Composed of Some Chemically Modified Hemes and Parallel G-Quadruplex DNAs

Biochemistry ◽  
2015 ◽  
Vol 54 (49) ◽  
pp. 7168-7177 ◽  
Author(s):  
Yasuhiko Yamamoto ◽  
Masashi Kinoshita ◽  
Yuya Katahira ◽  
Haruna Shimizu ◽  
Yue Di ◽  
...  
Keyword(s):  
Dna Complexes ◽  
Chemically Modified ◽  
G Quadruplex

Characterization of Bacteriophage T7-Induced DNA Primase

DNA Synthesis ◽  
1978 ◽  
pp. 655-667
Author(s):  
Eberhard Scherzinger ◽  
Günter Hillenbrand ◽  
Atsushi Yuki ◽  
Erich Lanka
Keyword(s):  
Bacteriophage T7 ◽  
Dna Primase

Characterization of structure and mechanism of transfection-active peptide–DNA complexes

2002 ◽  
Vol 1576 (1-2) ◽  
pp. 45-52 ◽  
Author(s):  
Roman Dallüge ◽  
Annekathrin Haberland ◽  
Sergei Zaitsev ◽  
Marc Schneider ◽  
Heidi Zastrow ◽  
...  
Keyword(s):  
Dna Complexes ◽  
Active Peptide

Isolation and characterization of mutant bacteriophage T7 RNA polymerases

1992 ◽  
Vol 224 (2) ◽  
pp. 307-318 ◽  
Author(s):  
Debabrata Patra ◽  
Eileen M. Lafer ◽  
Rui Sousa
Keyword(s):  
Rna Polymerases ◽  
Bacteriophage T7 ◽  
Isolation And Characterization

Biophysical Characterization of Cationic Liposome–DNA Complexes and their Interaction with Cells

2003 ◽  
pp. 298-312 ◽  
Author(s):  
Maria C Pedroso de Lima ◽  
Henrique Faneca ◽  
Miguel Mano ◽  
Nuno Penacho ◽  
Nejat Düzgüneş ◽  
...  
Keyword(s):  
Cationic Liposome ◽  
Dna Complexes ◽  
Biophysical Characterization

scholarly journals Structural and Biochemical Characterization of Phage λ FI Protein (gpFI) Reveals a Novel Mechanism of DNA Packaging Chaperone Activity

2012 ◽  
Vol 287 (38) ◽  
pp. 32085-32095 ◽  
Author(s):  
Ana Popovic ◽  
Bin Wu ◽  
Cheryl H. Arrowsmith ◽  
Aled M. Edwards ◽  
Alan R. Davidson ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Dna Packaging ◽  
Chaperone Activity
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