Selection for temperature-sensitive mutations in specific vaccinia virus genes: Isolation and characterization of a virus mutant which encodes a phosphonoacetic acid-resistant, temperature-sensitive DNA polymerase

Virology ◽  
1983 ◽  
Vol 128 (2) ◽  
pp. 444-457 ◽  
Author(s):  
P. Sridhar ◽  
Richard C. Condit
Keyword(s):  
Vaccinia Virus ◽  
Dna Polymerase ◽  
Temperature Sensitive ◽  
Phosphonoacetic Acid ◽  
Isolation And Characterization ◽  
Selection For ◽  
Virus Mutant

scholarly journals Characterization of the genomic RNA from a Rous sarcoma virus mutant temperature sensitive for cell transformation

1979 ◽  
Vol 6 (2) ◽  
pp. 471-486 ◽  
Author(s):  
Jean-Luc Darlix ◽  
Mireille Levray ◽  
Peter A. Bromley ◽  
Pierre-François Spahr
Keyword(s):  
Rous Sarcoma Virus ◽  
Cell Transformation ◽  
Temperature Sensitive ◽  
Genomic Rna ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Virus Mutant

Isolation and characterization of a variant myoblast cell line that is temperature sensitive for differentiation

1988 ◽  
Vol 8 (6) ◽  
pp. 2335-2341
Author(s):  
R J Akhurst ◽  
N B Flavin ◽  
J Worden
Keyword(s):  
Cell Line ◽  
Progenitor Cell ◽  
Temperature Shift ◽  
Temperature Sensitive ◽  
Isolation And Characterization ◽  
New Variant ◽  
Myoblast Cell Line ◽  
Myoblast Cell ◽  
Progenitor Cell Line

A new variant rat myogenic cell line, ts485, was isolated by subcloning the cell line ts3b2 (H. T. Nguyen, R. M. Medford, and B. Nadal-Ginard, Cell 34:281-293, 1983). Unlike the progenitor cell line, ts485 was thermosensitive for differentiation. Experiments with conditioned medium suggested that diffusible extracellular factors were not involved in dictating the differential phenotypes of ts485 cells cultured at the permissive and nonpermissive temperatures. Temperature shift experiments performed on cultures of ts485 cells indicated that the temperature-sensitive lesion was in a factor active during the growth phase and required to trigger a cascade of events leading to terminal differentiation.

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