Isolation and characterization of a Chinese hamster ovary mutant cell line with altered sensitivity to vaccinia virus killing.

1996 ◽  
Vol 70 (7) ◽  
pp. 4655-4666 ◽  
Author(s):  
C H Bair ◽  
C S Chung ◽  
I A Vasilevskaya ◽  
W Chang
Keyword(s):  
Vaccinia Virus ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Mutant Cell ◽  
Chinese Hamster ◽  
Mutant Cell Line ◽  
Isolation And Characterization ◽  
Altered Sensitivity

Isolation and characterization of Chinese hamster ovary cell mutants with altered sensitivity to high doses of tunicamycin

1981 ◽  
Vol 7 (5) ◽  
pp. 507-521 ◽  
Author(s):  
Michihiko Kuwano ◽  
Toshiko Tabuki ◽  
Shin-ichi Akiyama ◽  
Kumato Mifune ◽  
Akira Takatsuki ◽  
...  
Keyword(s):  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Isolation And Characterization ◽  
High Doses ◽  
Altered Sensitivity

scholarly journals Isolation and Characterization of a Mutant Chinese Hamster Ovary Cell Line That Is Resistant to Chlamydia trachomatis Infection at a Novel Step in the Attachment Process

2001 ◽  
Vol 69 (9) ◽  
pp. 5899-5904 ◽  
Author(s):  
Reynaldo A. Carabeo ◽  
Ted Hackstadt
Keyword(s):  
Chlamydia Trachomatis ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Mutant Cell ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Chemical Mutagenesis ◽  
Chlamydia Trachomatis Infection ◽  
Isolation And Characterization ◽  
Ovary Cell Line

ABSTRACT Host factors involved in Chlamydia trachomatispathogenesis were investigated by random chemical mutagenesis of Chinese hamster ovary (CHO-K1) cells followed by selection for clones resistant to chlamydial infection. A clonal mutant cell line, D4.1–3, refractory to infection by the C. trachomatis L2 serovar was isolated. The D4.1–3 cell line appears to be lacking in a previously undescribed temperature-dependent and heparin-resistant binding step that occurs subsequent to engagement of cell surface heparan sulfate by L2 elementary bodies. This novel binding step differentiates the lymphogranuloma venereum (LGV) serovar from other serovars and may contribute the different pathologies associated with LGV and non-LGV strains.

scholarly journals Synthesis and biochemical properties of a new photoactivatable cholesterol analog 7,7-azocholestanol and its linoleate ester in Chinese hamster ovary cell lines

2002 ◽  
Vol 43 (8) ◽  
pp. 1341-1347 ◽  
Author(s):  
Jonathan C. Cruz ◽  
Matthew Thomas ◽  
Edmund Wong ◽  
Nobutaka Ohgami ◽  
Shigeki Sugii ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Chinese Hamster Ovary ◽  
Cholesterol Metabolism ◽  
Mutant Cell ◽  
Chinese Hamster ◽  
Biochemical Properties ◽  
Ovary Cell ◽  
Cyclodextrin Complex ◽  
Mutant Cell Line

We report the chemical synthesis of a new photoactivatable cholesterol analog 7,7-azocholestanol (AC) and its linoleate ester (ACL). We also examined the biochemical properties of the sterol and its ester by employing several different mutant Chinese hamster ovary (CHO) cell lines with defined abnormalities in cholesterol metabolism as tools. AC mimics cholesterol in supporting the growth of a mutant cell line (M19) that requires cholesterol for growth. In normal cells, tritiated ACL present in low-density lipoprotein (LDL) was hydrolyzed and reesterified in a manner similar to tritiated cholesteryl linoleate (CL) in LDL. Also, in the mutant cell line (AC29) lacking the enzyme acyl-coenzyme A:cholesterol acyltransferase or in the mutant cell line (CT60) defective in the Niemann-Pick type C1 protein, the hydrolysis of ACL in LDL was normal, but the reesterification of the liberated AC was defective. Therefore, the metabolism of ACL in LDL is very similar to that of CL in LDL. Tritium-labeled AC delivered to intact CHO cells as a cyclodextrin complex was shown to photoaffinity label several discrete polypeptides, including caveolin-1.These results demonstrate AC as an effective reagent for studying cholesterol-protein interactions involved in intracellular cholesterol trafficking.

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