Immunological analysis of 140-kDa adenovirus-encoded DNA polymerase in adenovirus type 2-infected HeLa cells using antibodies raised against the protein expressed in Escherichia Coli

Virology ◽  
1987 ◽  
Vol 160 (2) ◽  
pp. 389-399 ◽  
Author(s):  
Yasuyuki Sasaguri ◽  
Thomas Sanford ◽  
Penny Aguirre ◽  
Radha Padmanabhan
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Hela Cells ◽  
Adenovirus Type ◽  
Immunological Analysis ◽  
Adenovirus Type 2

Visualization of genetic recombination intermediates of human adenovirus type 2 DNA from infected HeLa cells

Nature ◽  
1980 ◽  
Vol 287 (5778) ◽  
pp. 168-171 ◽  
Author(s):  
Debra J. Wolgemuth ◽  
Ming-Ta Hsu
Keyword(s):  
Hela Cells ◽  
Genetic Recombination ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Adenovirus Type 2

scholarly journals Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus

1990 ◽  
Vol 18 (5) ◽  
pp. 1167-1173 ◽  
Author(s):  
Christine J. Watson ◽  
Ronald T. Hay
Keyword(s):  
Dna Polymerase ◽  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Adenovirus Type ◽  
Adenovirus Type 2

Expression of protein IIIa of human adenovirus type 2 in Escherichia coli

Gene ◽  
1987 ◽  
Vol 55 (2-3) ◽  
pp. 295-301 ◽  
Author(s):  
M. Cuillel ◽  
M. Milleville ◽  
J.C. D'Halluin
Keyword(s):  
Escherichia Coli ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Adenovirus Type 2

Synthesis of human adenovirus type 2 fiber protein in Escherichia coli cells

Gene ◽  
1989 ◽  
Vol 81 (2) ◽  
pp. 267-274 ◽  
Author(s):  
Christine Chatellard ◽  
Jadwiga Chroboczek
Keyword(s):  
Escherichia Coli ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Escherichia Coli Cells ◽  
Fiber Protein ◽  
Adenovirus Type 2

scholarly journals Mutations in two cysteine-histidine-rich clusters in adenovirus type 2 DNA polymerase affect DNA binding.

1992 ◽  
Vol 66 (10) ◽  
pp. 5788-5796 ◽  
Author(s):  
I Joung ◽  
J A Engler
Keyword(s):  
Dna Binding ◽  
Dna Polymerase ◽  
Adenovirus Type ◽  
Adenovirus Type 2

Interaction between HeLa cells and adenovirus type 2 virions neutralized by different antisera.

1985 ◽  
Vol 56 (3) ◽  
pp. 896-903 ◽  
Author(s):  
C E Wohlfart ◽  
U K Svensson ◽  
E Everitt
Keyword(s):  
Hela Cells ◽  
Adenovirus Type ◽  
Adenovirus Type 2

scholarly journals Gewinnung und Reinigung von Adenovirus-Typ 2 mit Hilfe der CsCl-Gradientenzentrifugation

1962 ◽  
Vol 17 (8) ◽  
pp. 544-547 ◽  
Author(s):  
Kurt Köhler
Keyword(s):  
Sedimentation Rate ◽  
Hela Cells ◽  
Gradient Centrifugation ◽  
Adenovirus Type ◽  
Electron Microscopic ◽  
Cscl Gradient ◽  
Plaque Test ◽  
Microscopic Investigations ◽  
Adenovirus Type 2

Adenovirus Type 2 was grown on HeLa cells and purified by CsCl-gradient centrifugation. Three regions of bands were obtained, differing by their sedimentation rate and density in CsCl solutions. Electron microscopic investigations of negatively stained preparations show icosahedral particles with 252 subunits for the main virus region. The lighter bands consist of empty shells. A plaque test suiting the conditions was developed.

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