Filamentous phage pre-coat is an integral membrane protein: Analysis by a new method of membrane preparation

Cell ◽  
1982 ◽  
Vol 28 (1) ◽  
pp. 177-184 ◽  
Author(s):  
Marjorie Russel ◽  
Peter Model
Keyword(s):  
Membrane Protein ◽  
Protein Analysis ◽  
Integral Membrane Protein ◽  
New Method ◽  
Membrane Preparation ◽  
Filamentous Phage

scholarly journals Predicting the Structure and Dynamics of Membrane Protein GerAB from Bacillus subtilis

2021 ◽  
Vol 22 (7) ◽  
pp. 3793
Author(s):  
Sophie Blinker ◽  
Jocelyne Vreede ◽  
Peter Setlow ◽  
Stanley Brul
Keyword(s):  
Bacillus Subtilis ◽  
Membrane Protein ◽  
Spore Germination ◽  
Structural Information ◽  
Transmembrane Protein ◽  
Homology Modelling ◽  
Water Channel ◽  
Md Simulations ◽  
Integral Membrane Protein ◽  
Starting Point

Bacillus subtilis forms dormant spores upon nutrient depletion. Germinant receptors (GRs) in spore’s inner membrane respond to ligands such as L-alanine, and trigger spore germination. In B. subtilis spores, GerA is the major GR, and has three subunits, GerAA, GerAB, and GerAC. L-Alanine activation of GerA requires all three subunits, but which binds L-alanine is unknown. To date, how GRs trigger germination is unknown, in particular due to lack of detailed structural information about B subunits. Using homology modelling with molecular dynamics (MD) simulations, we present structural predictions for the integral membrane protein GerAB. These predictions indicate that GerAB is an α-helical transmembrane protein containing a water channel. The MD simulations with free L-alanine show that alanine binds transiently to specific sites on GerAB. These results provide a starting point for unraveling the mechanism of L-alanine mediated signaling by GerAB, which may facilitate early events in spore germination.

Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein fromEscherichia coli

1998 ◽  
Vol 54 (4) ◽  
pp. 697-699 ◽  
Author(s):  
Barbara S. Smith ◽  
Bostjan Kobe ◽  
Ravi Kurumbail ◽  
Susan K. Buchanan ◽  
Lalitha Venkatramani ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Integral Membrane Protein ◽  
X Ray ◽  
Fromescherichia Coli
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