Incomplete tolerance induced in Xenopus by larval tissue allografting: Evidence from immunohistology and mixed leucocyte culture

1993 ◽  
Vol 17 (3) ◽  
pp. 249-262 ◽  
Author(s):  
John D. Horton ◽  
Trudy L. Horton ◽  
Pamela Ritchie
Keyword(s):  
Leucocyte Culture ◽  
Larval Tissue

Leucocyte Culture and Chromosome Preparations from Pig Blood

1968 ◽  
Vol 43 (4) ◽  
pp. 187-190 ◽  
Author(s):  
Probodh K. Srivastava ◽  
J. F. Lasley
Keyword(s):  
Leucocyte Culture

scholarly journals Novel applications of acrylamide for cryosectioning of isolated cells, tissues, and arthropods.

1991 ◽  
Vol 39 (9) ◽  
pp. 1289-1293 ◽  
Author(s):  
D D Bronson ◽  
H J Bidmon ◽  
K H Tomaschko ◽  
W E Stumpf
Keyword(s):  
Soft Tissues ◽  
External Support ◽  
Xenopus Laevis Oocytes ◽  
Air Bubbles ◽  
Isolated Cells ◽  
Sarcophaga Bullata ◽  
Larval Tissue ◽  
Larval Insect ◽  
Novel Applications ◽  
Pycnogonum Litorale

Cross-linked and uncross-linked acrylamide polymers were used to alleviate technical difficulties in cryosectioning and autoradiographic processing of marine arthropods (Pycnogonum litorale), larval insect tissues (Sarcophaga bullata), and amphibian (Xenopus laevis) oocytes. Rapidly polymerized cross-linked acrylamide was used to prepare sections from an animal with a hard thick cuticle, P. litorale. Fragmentation and compression artifacts caused by tissue density differences were avoided and internal tissues were well preserved. Our results indicate that closely applied external support eliminates difficulties typically associated with arthropod cryosectioning. Pre-polymerized, uncross-linked acrylamide provided similar protection when used for sectioning larval tissue from S. bullata. Soft tissues under the cuticle were undamaged in tracheal and epidermal preparations. This polymer was also an excellent embedding material for soft tissues such as salivary glands, quickly penetrating tissue cavities and eliminating air bubbles. Uncross-linked acrylamide was used as an embedding and culturing medium for oocytes from X. laevis. The polymers were non-toxic and allowed the preparation of thin frozen sections containing as many as 50 large oocytes per section.

MICROTECHNIQUE FOR LEUCOCYTE CULTURE

The Lancet ◽  
1965 ◽  
Vol 285 (7400) ◽  
pp. 1396
Author(s):  
Shih Wen Huang
Keyword(s):  
Leucocyte Culture

Analysis of mixed leucocyte culture (MLC) reactive cells after in vitro priming

1975 ◽  
Vol 16 (1) ◽  
pp. 171-181 ◽  
Author(s):  
Reginald M. Gorczynski ◽  
Marvin B. Rittenberg
Keyword(s):  
Leucocyte Culture
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